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Literature summary for 1.3.1.77 extracted from

  • Xie, D.Y.; Sharma, S.B.; Dixon, R.A.
    Anthocyanidin reductases from Medicago truncatula and Arabidopsis thaliana (2004), Arch. Biochem. Biophys., 422, 91-102.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a fusion protein with maltose-binding protein Arabidopsis thaliana
expression in Escherichia coli as a fusion protein with maltose-binding protein Medicago truncatula

Inhibitors

Inhibitors Comment Organism Structure
(+)-catechin 0.5 mM, 50% inhibition Arabidopsis thaliana
(+/-)-dihydroquercetin 0.025 mM Arabidopsis thaliana
additional information no inhibition by Na+ up to 400 mM Arabidopsis thaliana
additional information no inhibition by (+)-catechin and (+/-)-dihydroquercetin Medicago truncatula
Na+ above 200 mM Medicago truncatula
quercetin strong inhibition Arabidopsis thaliana
quercetin strong inhibition Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
NADPH pH 6.0 Medicago truncatula
0.0145
-
pelargonidin pH 6.0 Medicago truncatula
0.94
-
NADH pH 6.0 Medicago truncatula
0.0498
-
delphinidin pH 6.0 Medicago truncatula
0.0528
-
pelargonidin pH 8.0 Arabidopsis thaliana
0.0178
-
delphinidin pH 8.0 Arabidopsis thaliana
0.0129
-
cyanidin pH 6.0 Medicago truncatula
0.0739
-
cyanidin pH 8.0 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
anthocyanidin + NAD(P)H Arabidopsis thaliana enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins 2,3-cis-flavan-3-ol + NAD(P)+
-
?
anthocyanidin + NAD(P)H Medicago truncatula enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins 2,3-cis-flavan-3-ol + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Medicago truncatula
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anthocyanidin + NAD(P)H enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins Arabidopsis thaliana 2,3-cis-flavan-3-ol + NAD(P)+
-
?
anthocyanidin + NAD(P)H enzyme of flavonoid pathway involved in the biosynthesis of condensed tannins Medicago truncatula 2,3-cis-flavan-3-ol + NAD(P)+
-
?
cyanidin + NADPH
-
Arabidopsis thaliana (-)-epicatechin + NAD(P)+
-
?
cyanidin + NADPH preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin Medicago truncatula (-)-epicatechin + NAD(P)+
-
?
delphinidin + 2 NADPH + H+
-
Arabidopsis thaliana (-)-epigallocatechin + 2 NADP+
-
?
delphinidin + 2 NADPH + H+ preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin Medicago truncatula (-)-epigallocatechin + 2 NADP+
-
?
pelargonidin + 2 NADPH + H+
-
Arabidopsis thaliana (-)-epiafzelechin + 2 NADP+
-
?
pelargonidin + 2 NADPH + H+ preference of anthocyanidin substrates in decreasing order: cyanidin, pelargonidin and delphinidin Medicago truncatula (-)-epiafzelechin + 2 NADP+
-
?
(-)-epicatechin + NADH + H+
-
Medicago truncatula cyanidin + NAD+
-
r

Synonyms

Synonyms Comment Organism
AtANR
-
Arabidopsis thaliana
MtANR
-
Medicago truncatula
ANR
-
Arabidopsis thaliana
ANR
-
Medicago truncatula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Medicago truncatula
55
-
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
cyanidin pH 6.0 Medicago truncatula
0.058
-
delphinidin pH 8.0 Arabidopsis thaliana
1.34
-
delphinidin pH 6.0 Medicago truncatula
1.045
-
cyanidin pH 8.0 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Arabidopsis thaliana
6
-
in 50 mM MES buffer Medicago truncatula
5.5
-
in 50 mM citrate/phosphate buffer Medicago truncatula

pH Range

pH Minimum pH Maximum Comment Organism
7 8.9 pH 7.0: about 40% of maximal activity, pH 8.9: about 90% of maximal activity Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH slight preference for NADPH over NADH Arabidopsis thaliana
NADPH slight preference for NADPH over NADH Medicago truncatula
NADH slight preference for NADPH over NADH Arabidopsis thaliana
NADH slight preference for NADPH over NADH Medicago truncatula