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Literature summary for 1.3.1.74 extracted from

  • Yamauchi, Y.; Hasegawa, A.; Taninaka, A.; Mizutani, M.; Sugimoto, Y.
    NADPH-dependent reductases involved in the detoxification of reactive carbonyls in plants (2011), J. Biol. Chem., 286, 6999-7009.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.74

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli as a GST-fusion protein Arabidopsis thaliana
overexpressed in Escherichia coli as a GST-fusion protein Cucumis sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH pH 7.0, 25°C Arabidopsis thaliana
0.023
-
 4-hexen-3-one pH 7.0, 25°C Cucumis sativus
0.053
-
 NADH pH 7.0, 25°C Arabidopsis thaliana
0.12
-
 4-hexen-3-one pH 7.0, 25°C Arabidopsis thaliana
0.13
-
 3-buten-2-one pH 7.0, 25°C Arabidopsis thaliana
0.185
-
 NADPH pH 7.0, 25°C Cucumis sativus
0.29
-
 1-penten-3-one pH 7.0, 25°C Arabidopsis thaliana
0.58
-
 1-penten-3-one pH 7.0, 25°C Cucumis sativus
0.63
-
 3-penten-2-one pH 7.0, 25°C Cucumis sativus
1.8
-
 crotonaldehyde pH 7.0, 25°C Arabidopsis thaliana
2.88
-
 3-buten-2-one pH 7.0, 25°C Cucumis sativus
3.5
-
 propenal pH 7.0, 25°C Arabidopsis thaliana
4.64
-
 NADH pH 7.0, 25°C Cucumis sativus
13.6
-
 propenal pH 7.0, 25°C Cucumis sativus
29.8
-
 crotonaldehyde pH 7.0, 25°C Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-
chloroplast
-
 Cucumis sativus 9507
-
cytosol
-
 Cucumis sativus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE Arabidopsis thaliana
42000
-
 SDS-PAGE Cucumis sativus

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Cucumis sativus E2S0A5 chloroplast variant
-
Cucumis sativus E2S0A6 cytosolic variant
-

Purification (Commentary)

Purification (Comment) Organism
using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration Arabidopsis thaliana
using anion exchange chromatography, hydroxyapatite, phenyl-Sepharose, and gel filtration Cucumis sativus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Cucumis sativus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-penten-3-one + NADH
-
 Arabidopsis thaliana ? + NAD+
-
 ?
1-penten-3-one + NADH
-
 Cucumis sativus ? + NAD+
-
 ?
1-penten-3-one + NADH
-
 Cucumis sativus pentan-3-one + NAD+
-
 ?
1-penten-3-one + NADPH
-
 Cucumis sativus ? + NADP+
-
 ?
1-penten-3-one + NADPH
-
 Arabidopsis thaliana pentan-3-one + NADP+
-
 ?
1-penten-3-one + NADPH
-
 Cucumis sativus pentan-3-one + NADP+
-
 ?
3-buten-2-one + NADH
-
 Arabidopsis thaliana 2-butanone + NAD+
-
 ?
3-buten-2-one + NADH
-
 Cucumis sativus 2-butanone + NAD+
-
 ?
3-buten-2-one + NADPH
-
 Arabidopsis thaliana 2-butanone + NADP+
-
 ?
3-buten-2-one + NADPH
-
 Cucumis sativus 2-butanone + NADP+
-
 ?
3-penten-2-one + NADH
-
 Cucumis sativus 2-pentanone + NAD+
-
 ?
3-penten-2-one + NADPH
-
 Cucumis sativus 2-pentanone + NADP+
-
 ?
4-hexen-3-one + NADH
-
 Arabidopsis thaliana hexan-3-one + NAD+
-
 ?
4-hexen-3-one + NADH
-
 Cucumis sativus hexan-3-one + NAD+
-
 ?
4-hexen-3-one + NADPH
-
 Arabidopsis thaliana hexan-3-one + NADP+
-
 ?
4-hexen-3-one + NADPH
-
 Cucumis sativus hexan-3-one + NADP+
-
 ?
crotonaldehyde + NADH
-
 Arabidopsis thaliana butanal + NAD+
-
 ?
crotonaldehyde + NADH
-
 Cucumis sativus butanal + NAD+
-
 ?
crotonaldehyde + NADPH
-
 Arabidopsis thaliana butanal + NADP+
-
 ?
crotonaldehyde + NADPH
-
 Cucumis sativus butanal + NADP+
-
 ?
propenal + NADH
-
 Arabidopsis thaliana propanal + NAD+
-
 ?
propenal + NADH
-
 Cucumis sativus propanal + NAD+
-
 ?
propenal + NADPH
-
 Arabidopsis thaliana propanal + NADP+
-
 ?
propenal + NADPH
-
 Cucumis sativus propanal + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Arabidopsis thaliana
monomer
-
 Cucumis sativus

Synonyms

Synonyms Comment Organism
alkenal/one oxidoreductase
-
 Arabidopsis thaliana
alkenal/one oxidoreductase
-
 Cucumis sativus
AtAOR
-
 Arabidopsis thaliana
CsAOR
-
 Cucumis sativus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 crotonaldehyde pH 7.0, 25°C Arabidopsis thaliana
3.3
-
 4-hexen-3-one pH 7.0, 25°C Arabidopsis thaliana
3.5
-
 3-buten-2-one pH 7.0, 25°C Arabidopsis thaliana
4
-
 1-penten-3-one pH 7.0, 25°C Arabidopsis thaliana
22
-
 3-penten-2-one pH 7.0, 25°C Cucumis sativus
22
-
 propenal pH 7.0, 25°C Arabidopsis thaliana
23
-
 NADPH pH 7.0, 25°C Arabidopsis thaliana
23
-
 NADH pH 7.0, 25°C Arabidopsis thaliana
42
-
 crotonaldehyde pH 7.0, 25°C Cucumis sativus
61
-
 1-penten-3-one pH 7.0, 25°C Cucumis sativus
63
-
 propenal pH 7.0, 25°C Cucumis sativus
68
-
 4-hexen-3-one pH 7.0, 25°C Cucumis sativus
74
-
 3-buten-2-one pH 7.0, 25°C Cucumis sativus
75
-
 NADPH pH 7.0, 25°C Cucumis sativus
75
-
 NADH pH 7.0, 25°C Cucumis sativus

Cofactor

Cofactor Comment Organism Structure
NADH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Arabidopsis thaliana
NADH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Cucumis sativus
NADPH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Arabidopsis thaliana
NADPH enzyme operates 30times faster in the presence of NADPH than in the presence of NADH Cucumis sativus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.63
-
 propenal pH 7.0, 25°C Cucumis sativus
6.28
-
 propenal pH 7.0, 25°C Arabidopsis thaliana
13.8
-
 1-penten-3-one pH 7.0, 25°C Arabidopsis thaliana
16.2
-
 NADH pH 7.0, 25°C Cucumis sativus
25.7
-
 3-buten-2-one pH 7.0, 25°C Cucumis sativus
26.9
-
 3-buten-2-one pH 7.0, 25°C Arabidopsis thaliana
27.5
-
 4-hexen-3-one pH 7.0, 25°C Arabidopsis thaliana
34.9
-
 3-penten-2-one pH 7.0, 25°C Cucumis sativus
105
-
 1-penten-3-one pH 7.0, 25°C Cucumis sativus
406
-
 NADPH pH 7.0, 25°C Cucumis sativus
434
-
 NADH pH 7.0, 25°C Arabidopsis thaliana
2300
-
 NADPH pH 7.0, 25°C Arabidopsis thaliana
2960
-
 4-hexen-3-one pH 7.0, 25°C Cucumis sativus