BRENDA - Enzyme Database
show all sequences of 1.3.1.74

Molecular cloning and characterization of Dbr1, a 2-alkenal reductase from Artemisia annua

Zhang, Y.; Teoh, K.; Reed, D.; Covello, P.; Botany 87, 643-649 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Artemisia annua
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0064
-
2E-nonenal
pH 7.0, 30C
Artemisia annua
0.071
-
artemisinic aldehyde
pH 7.0, 30C
Artemisia annua
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
x * 38500, calculated
Artemisia annua
Organism
Organism
UniProt
Commentary
Textmining
Artemisia annua
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
Dbr1 expression is readily detectable in aerial parts of the plant, but not in the roots
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E)-4-hydroxynon-2-enal + NADPH + H+
-
697015
Artemisia annua
4-hydroxynonanal + NADP+
-
-
-
?
2E-hexenal + NADPH + H+
at 12% of the rate with 2E-nonenal
697015
Artemisia annua
hexanal + NADP+
-
-
-
?
2E-nonenal + NADPH + H+
-
697015
Artemisia annua
nonanal + NADP+
-
-
-
?
artemisinic aldehyde + NADPH + H+
at 1.9% of the rate with 2E-nonenal
697015
Artemisia annua
dihydroartemisinic aldehyde + NADP+
88% 11S- and 12% 11R-isomer
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38500, calculated
Artemisia annua
Synonyms
Synonyms
Commentary
Organism
Dbr1
-
Artemisia annua
double bond reductase
-
Artemisia annua
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Artemisia annua
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADH
Artemisia annua
NADPH
-
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADH
Artemisia annua
NADPH
-
Artemisia annua
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0064
-
2E-nonenal
pH 7.0, 30C
Artemisia annua
0.071
-
artemisinic aldehyde
pH 7.0, 30C
Artemisia annua
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
x * 38500, calculated
Artemisia annua
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
Dbr1 expression is readily detectable in aerial parts of the plant, but not in the roots
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E)-4-hydroxynon-2-enal + NADPH + H+
-
697015
Artemisia annua
4-hydroxynonanal + NADP+
-
-
-
?
2E-hexenal + NADPH + H+
at 12% of the rate with 2E-nonenal
697015
Artemisia annua
hexanal + NADP+
-
-
-
?
2E-nonenal + NADPH + H+
-
697015
Artemisia annua
nonanal + NADP+
-
-
-
?
artemisinic aldehyde + NADPH + H+
at 1.9% of the rate with 2E-nonenal
697015
Artemisia annua
dihydroartemisinic aldehyde + NADP+
88% 11S- and 12% 11R-isomer
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38500, calculated
Artemisia annua
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Artemisia annua
Other publictions for EC 1.3.1.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725462
Yamauchi
NADPH-dependent reductases inv ...
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2
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15
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6
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2
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2
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34
2
6
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4
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3
6
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3
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3
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23
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726133
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1
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697015
Zhang
-
Molecular cloning and characte ...
Artemisia annua
Botany
87
643-649
2009
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689624
Papdi
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1
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690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
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5
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676581
Mano
Protection against photooxidat ...
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Plant Physiol.
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1
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6
2
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4
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1
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6
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1
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6
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7
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655242
Dick
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1
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1
1
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7
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1
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Dick
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5
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1
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2
1
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1
1
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1
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1
5
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4
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1
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1
1
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636484
Mano
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1
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9
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1
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1
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1
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12
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9
1
1
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1
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1
1
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9
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12
-
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9
1
1
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1
12
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1
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4
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1
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4
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12
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