BRENDA - Enzyme Database show
show all sequences of 1.3.1.74

NADPH alkenal/one oxidoreductase activity determines sensitivity of cancer cells to the chemotherapeutic alkylating agent irofulven

Dick, R.A.; Yu, X.; Kensler, T.W.; Clin. Cancer Res. 10, 1492-1499 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in human 293 cells increases their sensitivity to irofulven by 100fold compared to control human 293 cells
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
illudin M
-
Rattus norvegicus
illudin S
-
Rattus norvegicus
irofulven
recombinant human 293 cells show 100fold increased sensitivity compared to the wild-type human 293 cells
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.109
-
illudin M
recombinant enzyme, pH 7.4, 37°C
Rattus norvegicus
0.145
-
irofulven
recombinant enzyme, pH 7.4, 37°C
Rattus norvegicus
0.308
-
illudin S
recombinant enzyme, pH 7.4, 37°C
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
illudin M + NADPH
Rattus norvegicus
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
?
-
-
?
illudin S + NADPH
Rattus norvegicus
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
?
-
-
?
irofulven + NADPH
Rattus norvegicus
less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.275
-
purified recombinant enzyme, substrate irofulven
Rattus norvegicus
44.1
-
purified recombinant enzyme, substrate illudin M
Rattus norvegicus
115.9
-
purified recombinant enzyme, substrate illudin S
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
illudin M + NADPH
-
655242
Rattus norvegicus
?
-
-
-
?
illudin M + NADPH
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
655242
Rattus norvegicus
?
-
-
-
?
illudin S + NADPH
-
655242
Rattus norvegicus
?
-
-
-
?
illudin S + NADPH
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
655242
Rattus norvegicus
?
-
-
-
?
irofulven + NADPH
-
655242
Rattus norvegicus
?
-
-
-
?
irofulven + NADPH
less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
655242
Rattus norvegicus
?
-
-
-
?
additional information
reaction product identification by mass spectrometry
655242
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in human 293 cells increases their sensitivity to irofulven by 100fold compared to control human 293 cells
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
illudin M
-
Rattus norvegicus
illudin S
-
Rattus norvegicus
irofulven
recombinant human 293 cells show 100fold increased sensitivity compared to the wild-type human 293 cells
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.109
-
illudin M
recombinant enzyme, pH 7.4, 37°C
Rattus norvegicus
0.145
-
irofulven
recombinant enzyme, pH 7.4, 37°C
Rattus norvegicus
0.308
-
illudin S
recombinant enzyme, pH 7.4, 37°C
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
illudin M + NADPH
Rattus norvegicus
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
?
-
-
?
illudin S + NADPH
Rattus norvegicus
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
?
-
-
?
irofulven + NADPH
Rattus norvegicus
less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
?
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.275
-
purified recombinant enzyme, substrate irofulven
Rattus norvegicus
44.1
-
purified recombinant enzyme, substrate illudin M
Rattus norvegicus
115.9
-
purified recombinant enzyme, substrate illudin S
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
illudin M + NADPH
-
655242
Rattus norvegicus
?
-
-
-
?
illudin M + NADPH
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
655242
Rattus norvegicus
?
-
-
-
?
illudin S + NADPH
-
655242
Rattus norvegicus
?
-
-
-
?
illudin S + NADPH
extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
655242
Rattus norvegicus
?
-
-
-
?
irofulven + NADPH
-
655242
Rattus norvegicus
?
-
-
-
?
irofulven + NADPH
less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme
655242
Rattus norvegicus
?
-
-
-
?
additional information
reaction product identification by mass spectrometry
655242
Rattus norvegicus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Rattus norvegicus
Other publictions for EC 1.3.1.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725462
Yamauchi
NADPH-dependent reductases inv ...
Arabidopsis thaliana, Cucumis sativus
J. Biol. Chem.
286
6999-7009
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2
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34
2
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3
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23
3
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3
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3
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3
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34
3
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13
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Arabidopsis thaliana
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27
193-197
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1
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1
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1
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697015
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1
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4
1
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1
2
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1
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1
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4
1
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1
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689624
Papdi
Functional identification of A ...
Arabidopsis thaliana
Plant Physiol.
147
528-542
2008
1
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-
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1
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2
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-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
5
-
1
-
-
-
6
1
1
6
1
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3
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1
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1
2
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1
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1
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5
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1
1
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6
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1
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1
2
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1
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674632
Yu
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Rattus norvegicus
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281
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2006
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1
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2
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4
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1
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3
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1
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1
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1
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1
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2
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3
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675296
Gong
Investigating the role of ster ...
Homo sapiens
J. Med. Chem.
49
2593-2599
2006
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1
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4
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1
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1
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4
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1
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1
1
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4
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1
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4
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676581
Mano
Protection against photooxidat ...
Arabidopsis thaliana
Plant Physiol.
139
1773-1783
2005
-
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1
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6
2
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4
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1
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6
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7
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1
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1
1
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6
2
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1
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6
-
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7
-
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-
-
-
-
655242
Dick
NADPH alkenal/one oxidoreducta ...
Rattus norvegicus
Clin. Cancer Res.
10
1492-1499
2004
-
-
1
-
-
-
3
3
-
-
-
3
-
3
-
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-
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3
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7
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1
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1
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1
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1
1
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3
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3
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3
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3
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7
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1
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1
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656210
Dick
The catalytic and kinetic mech ...
Rattus norvegicus
J. Biol. Chem.
279
17269-17277
2004
-
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4
5
-
1
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1
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3
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1
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4
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1
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1
1
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2
1
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2
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4
1
5
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1
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1
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4
-
1
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1
1
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636484
Mano
The NADPH:quinone oxidoreducta ...
Arabidopsis thaliana
Plant Cell Physiol.
43
1445-1455
2002
-
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1
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9
-
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1
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1
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1
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12
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9
1
1
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1
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1
1
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9
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1
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1
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12
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9
1
1
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390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
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1
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3
4
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1
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1
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1
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1
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1
12
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1
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4
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1
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1
1
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3
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4
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1
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1
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1
12
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1
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4
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390628
Stoffel
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
356
385-390
1975
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