BRENDA - Enzyme Database show
show all sequences of 1.3.1.74

The NADPH:quinone oxidoreductase P1-x-crystallin in Arabidopsis catalyzes the alpha,beta-hydrogenation of 2-alkenals: Detoxication of the lipid peroxide-derived reactive aldehydes

Mano, J.i.; Torii, Y.; Hayashi, S.i.; Takimoto, K.; Matsui, K.; Nakamura, K.; Inze, D.; Babiychuk, E.; Kushnir, S.; Asada, K.; Plant Cell Physiol. 43, 1445-1455 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
(2E)-nonenal
pH 6
Arabidopsis thaliana
0.0052
-
3-penten-2-one
pH 6
Arabidopsis thaliana
0.0134
-
4-hydroxy-(2E)-nonenal
pH 6
Arabidopsis thaliana
0.055
-
3-buten-2-one
pH 6
Arabidopsis thaliana
0.128
-
Diamide
pH 6
Arabidopsis thaliana
0.145
-
4-hydroxy-(2E)-hexenal
pH 6
Arabidopsis thaliana
0.232
-
(2E)-hexenal
pH 6
Arabidopsis thaliana
1.42
-
(2E)-pentenal
pH 6
Arabidopsis thaliana
4.65
-
propenal
pH 6
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
n-alkanal + NAD(P)+
Arabidopsis thaliana
role in detoxication
alk-2-enal + NAD(P)H
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
homogeneity
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E)-hexenal + NAD(P)H
-
636484
Arabidopsis thaliana
n-hexanal + NAD(P)+
-
636484
Arabidopsis thaliana
?
(2E)-nonenal + NAD(P)H
-
636484
Arabidopsis thaliana
n-nonanal + NAD(P)+
-
-
-
?
(2E)-pentenal + NAD(P)H
-
636484
Arabidopsis thaliana
n-pentanal + NAD(P)+
-
-
-
?
3-buten-2-one + NAD(P)H
-
636484
Arabidopsis thaliana
2-butanone + NAD(P)+
-
-
-
?
3-penten-2-one + NAD(P)H
-
636484
Arabidopsis thaliana
2-pentanone + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-hexenal + NAD(P)H
-
636484
Arabidopsis thaliana
4-hydroxyhexenal + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
-
636484
Arabidopsis thaliana
4-hydroxynonylaldehyde + NAD(P)+
-
636484
Arabidopsis thaliana
?
diamide + NAD(P)H
-
636484
Arabidopsis thaliana
? + NAD(P)+
-
-
-
?
additional information
catalyzes the hydrogenation of alpha,beta-unsaturated bonds, but not the reduction of the aldehyde moiety in 2-alkenal molecules
636484
Arabidopsis thaliana
?
-
-
-
-
n-alkanal + NAD(P)+
carbon chain length C3-C9 serves as substrates
636484
Arabidopsis thaliana
alk-2-enal + NAD(P)H
-
-
-
?
n-alkanal + NAD(P)+
role in detoxication
636484
Arabidopsis thaliana
alk-2-enal + NAD(P)H
-
-
-
-
propenal + NAD(P)H
synonym: acrolein
636484
Arabidopsis thaliana
propanal + NAD(P)+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.583
-
(2E)-pentenal
pH 6
Arabidopsis thaliana
0.667
-
propenal
pH 6
Arabidopsis thaliana
0.7
-
4-hydroxy-(2E)-hexenal
pH 6
Arabidopsis thaliana
0.85
-
(2E)-nonenal
pH 6
Arabidopsis thaliana
1.05
-
(2E)-hexenal
pH 6
Arabidopsis thaliana
1.38
-
3-buten-2-one
pH 6
Arabidopsis thaliana
1.47
-
4-hydroxy-(2E)-nonenal
pH 6
Arabidopsis thaliana
1.58
-
Diamide
pH 6
Arabidopsis thaliana
1.72
-
3-penten-2-one
pH 6
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.3
6.6
50% activity at pH 5.3 and pH 6.6
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
(2E)-nonenal
pH 6
Arabidopsis thaliana
0.0052
-
3-penten-2-one
pH 6
Arabidopsis thaliana
0.0134
-
4-hydroxy-(2E)-nonenal
pH 6
Arabidopsis thaliana
0.055
-
3-buten-2-one
pH 6
Arabidopsis thaliana
0.128
-
Diamide
pH 6
Arabidopsis thaliana
0.145
-
4-hydroxy-(2E)-hexenal
pH 6
Arabidopsis thaliana
0.232
-
(2E)-hexenal
pH 6
Arabidopsis thaliana
1.42
-
(2E)-pentenal
pH 6
Arabidopsis thaliana
4.65
-
propenal
pH 6
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
n-alkanal + NAD(P)+
Arabidopsis thaliana
role in detoxication
alk-2-enal + NAD(P)H
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
homogeneity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E)-hexenal + NAD(P)H
-
636484
Arabidopsis thaliana
n-hexanal + NAD(P)+
-
636484
Arabidopsis thaliana
?
(2E)-nonenal + NAD(P)H
-
636484
Arabidopsis thaliana
n-nonanal + NAD(P)+
-
-
-
?
(2E)-pentenal + NAD(P)H
-
636484
Arabidopsis thaliana
n-pentanal + NAD(P)+
-
-
-
?
3-buten-2-one + NAD(P)H
-
636484
Arabidopsis thaliana
2-butanone + NAD(P)+
-
-
-
?
3-penten-2-one + NAD(P)H
-
636484
Arabidopsis thaliana
2-pentanone + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-hexenal + NAD(P)H
-
636484
Arabidopsis thaliana
4-hydroxyhexenal + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
-
636484
Arabidopsis thaliana
4-hydroxynonylaldehyde + NAD(P)+
-
636484
Arabidopsis thaliana
?
diamide + NAD(P)H
-
636484
Arabidopsis thaliana
? + NAD(P)+
-
-
-
?
additional information
catalyzes the hydrogenation of alpha,beta-unsaturated bonds, but not the reduction of the aldehyde moiety in 2-alkenal molecules
636484
Arabidopsis thaliana
?
-
-
-
-
n-alkanal + NAD(P)+
carbon chain length C3-C9 serves as substrates
636484
Arabidopsis thaliana
alk-2-enal + NAD(P)H
-
-
-
?
n-alkanal + NAD(P)+
role in detoxication
636484
Arabidopsis thaliana
alk-2-enal + NAD(P)H
-
-
-
-
propenal + NAD(P)H
synonym: acrolein
636484
Arabidopsis thaliana
propanal + NAD(P)+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.583
-
(2E)-pentenal
pH 6
Arabidopsis thaliana
0.667
-
propenal
pH 6
Arabidopsis thaliana
0.7
-
4-hydroxy-(2E)-hexenal
pH 6
Arabidopsis thaliana
0.85
-
(2E)-nonenal
pH 6
Arabidopsis thaliana
1.05
-
(2E)-hexenal
pH 6
Arabidopsis thaliana
1.38
-
3-buten-2-one
pH 6
Arabidopsis thaliana
1.47
-
4-hydroxy-(2E)-nonenal
pH 6
Arabidopsis thaliana
1.58
-
Diamide
pH 6
Arabidopsis thaliana
1.72
-
3-penten-2-one
pH 6
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.3
6.6
50% activity at pH 5.3 and pH 6.6
Arabidopsis thaliana
Other publictions for EC 1.3.1.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725462
Yamauchi
NADPH-dependent reductases inv ...
Arabidopsis thaliana, Cucumis sativus
J. Biol. Chem.
286
6999-7009
2011
-
-
2
-
-
-
-
15
3
-
2
-
-
6
-
-
2
-
-
2
-
-
34
2
-
-
-
15
-
-
-
4
-
-
-
-
-
3
6
-
-
-
-
-
-
23
3
-
3
-
-
-
-
3
-
3
-
-
34
3
-
-
-
23
-
-
-
-
-
-
-
-
13
20
726133
Mano
-
Accumulation of lipid peroxide ...
Arabidopsis thaliana
Plant Biotechnol.
27
193-197
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
697015
Zhang
-
Molecular cloning and characte ...
Artemisia annua
Botany
87
643-649
2009
-
-
1
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
689624
Papdi
Functional identification of A ...
Arabidopsis thaliana
Plant Physiol.
147
528-542
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
5
-
1
-
-
-
6
1
1
6
1
-
-
3
-
-
1
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
5
-
1
1
-
-
-
-
6
-
1
1
6
1
-
-
-
-
1
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674632
Yu
Nrf2-mediated induction of cyt ...
Rattus norvegicus
J. Biol. Chem.
281
26245-26252
2006
-
-
1
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
1
-
-
2
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
675296
Gong
Investigating the role of ster ...
Homo sapiens
J. Med. Chem.
49
2593-2599
2006
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676581
Mano
Protection against photooxidat ...
Arabidopsis thaliana
Plant Physiol.
139
1773-1783
2005
-
-
1
-
-
-
-
6
2
-
-
-
-
4
-
-
-
-
-
1
-
-
6
-
-
-
-
7
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
655242
Dick
NADPH alkenal/one oxidoreducta ...
Rattus norvegicus
Clin. Cancer Res.
10
1492-1499
2004
-
-
1
-
-
-
3
3
-
-
-
3
-
3
-
-
-
-
-
-
3
-
7
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
3
-
-
-
3
-
-
-
-
-
-
3
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656210
Dick
The catalytic and kinetic mech ...
Rattus norvegicus
J. Biol. Chem.
279
17269-17277
2004
-
-
-
-
-
-
4
5
-
1
-
1
-
3
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
1
-
2
1
-
-
-
-
-
2
-
-
-
-
4
1
5
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
636484
Mano
The NADPH:quinone oxidoreducta ...
Arabidopsis thaliana
Plant Cell Physiol.
43
1445-1455
2002
-
-
1
-
-
-
-
9
-
-
-
1
-
1
-
-
1
-
-
-
-
-
12
-
-
-
-
9
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
9
-
-
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
9
1
1
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
3
4
-
-
-
1
-
1
-
-
1
-
-
1
-
1
12
-
1
-
-
4
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
4
-
-
-
1
-
-
-
1
-
1
-
1
12
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
390628
Stoffel
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
356
385-390
1975
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
1
-
-
1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
-
3
-
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-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-