BRENDA - Enzyme Database show
show all sequences of 1.3.1.74

Antioxidative function and substrate specificity of NAD(P)H-dependent alkenal/one oxidoreductase. A new role for leukotriene B4 12-hydroxydehydrogenase/15-oxoprostaglandin 13-reductase

Dick, R.A.; Kwak, M.K.; Sutter, T.R.; Kensler, T.W.; J. Biol. Chem. 276, 40803-40810 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
cyclovalone
-
Rattus norvegicus
progesterone
-
Rattus norvegicus
quercetin
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
15-oxoprostaglandin E1
30C
Rattus norvegicus
0.11
-
ethyl vinyl ketone
30C
Rattus norvegicus
0.12
-
4-hydroxy-2-nonenal
30C
Rattus norvegicus
0.15
-
acrolein
30C
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
n-alkanal + NAD(P)+
Rattus norvegicus
role in detoxication
alk-2-enal + NAD(P)H
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Storage Stability
Storage Stability
Organism
-80C, no loss of activity for 8 months
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-oxoprostaglandin E1 + NAD(P)H
-
390830
Rattus norvegicus
? + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
-
390830
Rattus norvegicus
4-hydroxynonylaldehyde + NAD(P)+
-
-
-
?
benzylidine acetone + NAD(P)H
reduction of alpha,beta-double bond
390830
Rattus norvegicus
? + NAD(P)+
-
-
-
?
ethyl vinyl ketone + NAD(P)H
-
390830
Rattus norvegicus
diethylketone + NAD(P)+
-
-
-
?
methyl vinyl ketone + NAD(P)H
-
390830
Rattus norvegicus
dimethylketone + NAD(P)+
-
-
-
?
additional information
reduction of the alpha,beta-carbon-carbon double bond to a single bond, broad substrate specificity
390830
Rattus norvegicus
?
-
-
-
-
n-alkanal + NAD(P)+
-
390830
Rattus norvegicus
alk-2-enal + NAD(P)H
-
-
-
?
n-alkanal + NAD(P)+
role in detoxication
390830
Rattus norvegicus
alk-2-enal + NAD(P)H
-
-
-
-
propenal + NAD(P)H
synonym: acrolein
390830
Rattus norvegicus
propanal + NAD(P)+
-
-
-
?
trans,trans-2,4-alkadienal + NAD(P)H
-
390830
Rattus norvegicus
? + NAD(P)+
-
-
-
?
trans-2-alkenal + NAD(P)H
-
390830
Rattus norvegicus
alkanal + NAD(P)+
-
-
-
?
trans-4-hydroxy-2-alkenal + NAD(P)H
-
390830
Rattus norvegicus
4-hydroxyalkanal + NAD(P)+
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.67
-
acrolein
30C
Rattus norvegicus
40
-
15-oxoprostaglandin E1
30C
Rattus norvegicus
66.7
-
4-hydroxy-2-nonenal
30C
Rattus norvegicus
160
-
ethyl vinyl ketone
30C
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyclovalone
-
Rattus norvegicus
progesterone
-
Rattus norvegicus
quercetin
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.001
-
15-oxoprostaglandin E1
30C
Rattus norvegicus
0.11
-
ethyl vinyl ketone
30C
Rattus norvegicus
0.12
-
4-hydroxy-2-nonenal
30C
Rattus norvegicus
0.15
-
acrolein
30C
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
n-alkanal + NAD(P)+
Rattus norvegicus
role in detoxication
alk-2-enal + NAD(P)H
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Storage Stability (protein specific)
Storage Stability
Organism
-80C, no loss of activity for 8 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15-oxoprostaglandin E1 + NAD(P)H
-
390830
Rattus norvegicus
? + NAD(P)+
-
-
-
?
4-hydroxy-(2E)-nonenal + NAD(P)H
-
390830
Rattus norvegicus
4-hydroxynonylaldehyde + NAD(P)+
-
-
-
?
benzylidine acetone + NAD(P)H
reduction of alpha,beta-double bond
390830
Rattus norvegicus
? + NAD(P)+
-
-
-
?
ethyl vinyl ketone + NAD(P)H
-
390830
Rattus norvegicus
diethylketone + NAD(P)+
-
-
-
?
methyl vinyl ketone + NAD(P)H
-
390830
Rattus norvegicus
dimethylketone + NAD(P)+
-
-
-
?
additional information
reduction of the alpha,beta-carbon-carbon double bond to a single bond, broad substrate specificity
390830
Rattus norvegicus
?
-
-
-
-
n-alkanal + NAD(P)+
-
390830
Rattus norvegicus
alk-2-enal + NAD(P)H
-
-
-
?
n-alkanal + NAD(P)+
role in detoxication
390830
Rattus norvegicus
alk-2-enal + NAD(P)H
-
-
-
-
propenal + NAD(P)H
synonym: acrolein
390830
Rattus norvegicus
propanal + NAD(P)+
-
-
-
?
trans,trans-2,4-alkadienal + NAD(P)H
-
390830
Rattus norvegicus
? + NAD(P)+
-
-
-
?
trans-2-alkenal + NAD(P)H
-
390830
Rattus norvegicus
alkanal + NAD(P)+
-
-
-
?
trans-4-hydroxy-2-alkenal + NAD(P)H
-
390830
Rattus norvegicus
4-hydroxyalkanal + NAD(P)+
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.67
-
acrolein
30C
Rattus norvegicus
40
-
15-oxoprostaglandin E1
30C
Rattus norvegicus
66.7
-
4-hydroxy-2-nonenal
30C
Rattus norvegicus
160
-
ethyl vinyl ketone
30C
Rattus norvegicus
Other publictions for EC 1.3.1.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725462
Yamauchi
NADPH-dependent reductases inv ...
Arabidopsis thaliana, Cucumis sativus
J. Biol. Chem.
286
6999-7009
2011
-
-
2
-
-
-
-
15
3
-
2
-
-
6
-
-
2
-
-
2
-
-
34
2
-
-
-
15
-
-
-
4
-
-
-
-
-
3
6
-
-
-
-
-
-
23
3
-
3
-
-
-
-
3
-
3
-
-
34
3
-
-
-
23
-
-
-
-
-
-
-
-
13
20
726133
Mano
-
Accumulation of lipid peroxide ...
Arabidopsis thaliana
Plant Biotechnol.
27
193-197
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
697015
Zhang
-
Molecular cloning and characte ...
Artemisia annua
Botany
87
643-649
2009
-
-
1
-
-
-
-
2
-
-
1
-
-
1
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-
-
-
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1
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4
1
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1
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2
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1
2
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1
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1
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-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
689624
Papdi
Functional identification of A ...
Arabidopsis thaliana
Plant Physiol.
147
528-542
2008
1
-
-
-
-
-
-
-
-
-
-
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1
-
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2
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1
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-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
5
-
1
-
-
-
6
1
1
6
1
-
-
3
-
-
1
-
-
1
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
5
-
1
1
-
-
-
-
6
-
1
1
6
1
-
-
-
-
1
-
1
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674632
Yu
Nrf2-mediated induction of cyt ...
Rattus norvegicus
J. Biol. Chem.
281
26245-26252
2006
-
-
1
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
1
-
-
2
-
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
675296
Gong
Investigating the role of ster ...
Homo sapiens
J. Med. Chem.
49
2593-2599
2006
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676581
Mano
Protection against photooxidat ...
Arabidopsis thaliana
Plant Physiol.
139
1773-1783
2005
-
-
1
-
-
-
-
6
2
-
-
-
-
4
-
-
-
-
-
1
-
-
6
-
-
-
-
7
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
655242
Dick
NADPH alkenal/one oxidoreducta ...
Rattus norvegicus
Clin. Cancer Res.
10
1492-1499
2004
-
-
1
-
-
-
3
3
-
-
-
3
-
3
-
-
-
-
-
-
3
-
7
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
3
-
-
-
3
-
-
-
-
-
-
3
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656210
Dick
The catalytic and kinetic mech ...
Rattus norvegicus
J. Biol. Chem.
279
17269-17277
2004
-
-
-
-
-
-
4
5
-
1
-
1
-
3
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
1
-
2
1
-
-
-
-
-
2
-
-
-
-
4
1
5
-
1
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
636484
Mano
The NADPH:quinone oxidoreducta ...
Arabidopsis thaliana
Plant Cell Physiol.
43
1445-1455
2002
-
-
1
-
-
-
-
9
-
-
-
1
-
1
-
-
1
-
-
-
-
-
12
-
-
-
-
9
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
9
-
-
-
1
-
-
-
1
-
-
-
-
12
-
-
-
-
9
1
1
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
3
4
-
-
-
1
-
1
-
-
1
-
-
1
-
1
12
-
1
-
-
4
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
3
-
4
-
-
-
1
-
-
-
1
-
1
-
1
12
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
390628
Stoffel
Purification and characterizat ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
356
385-390
1975
-
-
-
-
-
-
-
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-
3
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1
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1
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1
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2
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-
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3
-
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1
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1
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2
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