BRENDA - Enzyme Database
show all sequences of 1.3.1.73

A newly-detected reductase from Rauvolfia closes a gap in the biosynthesis of the antiarrhythmic alkaloid ajmaline

Gao, S.; Von Schumann, G.; Stockigt, J.; Planta Med. 68, 906-911 (2002)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
x * 48000, SDS-PAGE
Rauvolfia serpentina
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1,2-dihydrovomilenine + NADPH + H+
Rauvolfia serpentina
enzyme is involved in the late stages of the biosynthesis of ajmaline
17-O-acetylnorajmaline + NADP+
-
Rauvolfia serpentina
?
Organism
Organism
UniProt
Commentary
Textmining
no activity in Catharanthus roseus
-
-
-
no activity in Gossypium hirsutum
-
-
-
no activity in Lonicera morrowii
-
-
-
no activity in Nicotiana tabacum
-
-
-
no activity in Saponaria officinalis
-
-
-
no activity in Solanum lycopersicum
-
-
-
Rauvolfia mannii
-
-
-
Rauvolfia mombasiana
-
-
-
Rauvolfia serpentina
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rauvolfia serpentina
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Rauvolfia serpentina
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rauvolfia serpentina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,2-dihydrovomilenine + NADPH + H+
-
440365
Rauvolfia serpentina
17-O-acetylnorajmaline + NADP+
-
440365
Rauvolfia serpentina
?
1,2-dihydrovomilenine + NADPH + H+
enzyme is involved in the late stages of the biosynthesis of ajmaline
440365
Rauvolfia serpentina
17-O-acetylnorajmaline + NADP+
-
440365
Rauvolfia serpentina
?
Subunits
Subunits
Commentary
Organism
?
x * 48000, SDS-PAGE
Rauvolfia serpentina
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Rauvolfia serpentina
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
stable
Rauvolfia serpentina
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Rauvolfia serpentina
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.2
7.7
50% of maximal activity at pH 5.2 and at pH 7.7
Rauvolfia serpentina
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.8
6.2
in 50 mM citric acid, 100 mM Na2HPO4 buffer, stable
Rauvolfia serpentina
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rauvolfia serpentina
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rauvolfia serpentina
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48000
-
x * 48000, SDS-PAGE
Rauvolfia serpentina
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1,2-dihydrovomilenine + NADPH + H+
Rauvolfia serpentina
enzyme is involved in the late stages of the biosynthesis of ajmaline
17-O-acetylnorajmaline + NADP+
-
Rauvolfia serpentina
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rauvolfia serpentina
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Rauvolfia serpentina
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,2-dihydrovomilenine + NADPH + H+
-
440365
Rauvolfia serpentina
17-O-acetylnorajmaline + NADP+
-
440365
Rauvolfia serpentina
?
1,2-dihydrovomilenine + NADPH + H+
enzyme is involved in the late stages of the biosynthesis of ajmaline
440365
Rauvolfia serpentina
17-O-acetylnorajmaline + NADP+
-
440365
Rauvolfia serpentina
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 48000, SDS-PAGE
Rauvolfia serpentina
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
-
Rauvolfia serpentina
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
stable
Rauvolfia serpentina
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Rauvolfia serpentina
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.2
7.7
50% of maximal activity at pH 5.2 and at pH 7.7
Rauvolfia serpentina
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.8
6.2
in 50 mM citric acid, 100 mM Na2HPO4 buffer, stable
Rauvolfia serpentina
Other publictions for EC 1.3.1.73
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673056
Gao
-
Biosynthesis of monoterpenoid ...
Rauvolfia serpentina
Chem. Res. Chin. Univ.
22
267-269
2006
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1
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1
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1
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1
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2
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2
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1
1
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1
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440365
Gao
A newly-detected reductase fro ...
no activity in Catharanthus roseus, no activity in Gossypium hirsutum, no activity in Lonicera morrowii, no activity in Nicotiana tabacum, no activity in Saponaria officinalis, no activity in Solanum lycopersicum, Rauvolfia mannii, Rauvolfia mombasiana, Rauvolfia serpentina
Planta Med.
68
906-911
2002
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1
1
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10
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1
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1
1
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2
1
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1
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1
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1
1
1
1
-
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1
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1
1
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1
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1
1
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2
1
1
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1
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1
1
1
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