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Literature summary for 1.3.1.73 extracted from
- A newly-detected reductase from Rauvolfia closes a gap in the biosynthesis of the antiarrhythmic alkaloid ajmaline (2002), Planta Med., 68, 906-911.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, SDS-PAGE | Rauvolfia serpentina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dihydrovomilenine + NADPH + H+ | Rauvolfia serpentina | enzyme is involved in the late stages of the biosynthesis of ajmaline | 17-O-acetylnorajmaline + NADP+ | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Catharanthus roseus | - |
- |
- |
| no activity in Gossypium hirsutum | - |
- |
- |
| no activity in Lonicera morrowii | - |
- |
- |
| no activity in Nicotiana tabacum | - |
- |
- |
| no activity in Saponaria officinalis | - |
- |
- |
| no activity in Solanum lycopersicum | - |
- |
- |
| Rauvolfia mannii | - |
- |
- |
| Rauvolfia mombasiana | - |
- |
- |
| Rauvolfia serpentina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rauvolfia serpentina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Rauvolfia serpentina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rauvolfia serpentina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dihydrovomilenine + NADPH + H+ | - |
Rauvolfia serpentina | 17-O-acetylnorajmaline + NADP+ | - |
? | |
| 1,2-dihydrovomilenine + NADPH + H+ | enzyme is involved in the late stages of the biosynthesis of ajmaline | Rauvolfia serpentina | 17-O-acetylnorajmaline + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, SDS-PAGE | Rauvolfia serpentina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rauvolfia serpentina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | stable | Rauvolfia serpentina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Rauvolfia serpentina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 7.7 | 50% of maximal activity at pH 5.2 and at pH 7.7 | Rauvolfia serpentina |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.2 | in 50 mM citric acid, 100 mM Na2HPO4 buffer, stable | Rauvolfia serpentina |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rauvolfia serpentina | |
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Release 2023.1 (January 2023)
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