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Literature summary for extracted from

  • Luu, W.; Hart-Smith, G.; Sharpe, L.J.; Brown, A.J.
    The terminal enzymes of cholesterol synthesis, DHCR24 and DHCR7, interact physically and functionally (2015), J. Lipid Res., 56, 888-897 .
    View publication on PubMedView publication on EuropePMC


Cloned (Comment) Organism
generation of plasmids pcDNA5-DHCR7-myc/FRT and pcDNA5-DHCR24-V5/FRT, and of DHCR24 mutant plasmid pcDNA5-DHCR24 Y471S-V5/FRT, the latter is stably recombinantly expressed in CHO-7 cells, stable overexpression of inactive DHCR24 mutant Y471S in CHO-7 cells, quantitative real-time PCR expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the DHCR24 gene is knocked down by siRNA, DHCR24 knockdown decreases DHCR24 activity almost completely in CHO-EV and CHO-DHCR24 Y471S compared with CHODHCR24 cells Homo sapiens
Y471S a desmosterolosis mutant of DHCR24 that results in a complete loss of DHCR24 activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesta-5,24-dien-3beta-ol + NADPH + H+ Homo sapiens
cholest-5-en-3beta-ol + NADP+


Organism UniProt Comment Textmining
Homo sapiens Q15392

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesta-5,24-dien-3beta-ol + NADPH + H+
Homo sapiens cholest-5-en-3beta-ol + NADP+


Synonyms Comment Organism
dehydrocholesterol reductase
Homo sapiens
Homo sapiens


Cofactor Comment Organism Structure
Homo sapiens

General Information

General Information Comment Organism
malfunction overexpression of DHCR24 enhances 7-dehydrocholesterol reductase, DHCR7, activity, but only when a functional form of DHCR24 is used. When the DHCR24 gene is knocked down by siRNA, DHCR7 activity is also ablated. Knockdown of DHCR7 has no effect on DHCR24 activity, while knockdown of DHCR24 decreases DHCR7 activity by about 60% Homo sapiens
metabolism substrate channeling in the cholesterol metabolon of choleterol biosynthesis, cholesterol synthesis proteins identified by LC-MS/MS after DHCR24 immunoprecipitation Homo sapiens
physiological function the enzyme DELTA24-sterol reductase DHCR24 is involved in the cholesterol biosynthesis catalyzing the reduction of desmosterol to cholesterol. DHCR24 controls the activity of 7-dehydrocholesterol , DHCR7, which is important for both cholesterol and vitamin D synthesis. DHCR24 is involved in a remarkable diversity of cellular functions (e.g., oxidative stress, neuroprotection, cell survival), and is implicated in many diseases including cardiovascular disease, hepatitis C, certain cancers, and neurodegenerative diseases Homo sapiens