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Literature summary for 1.3.1.72 extracted from

  • Fernandez, C.; Suarez, Y.; Ferruelo, A.J.; Gomez-Coronado, D.; Lasuncion, M.A.
    Inhibition of cholesterol biosynthesis by DELTA22-unsaturated phytosterols via competitive inhibition of sterol DELTA24-reductase in mammalian cells (2002), Biochem. J., 366, 109-119.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
5,22-cholestedien-3beta-ol desmosterol isomer, competitive inhibition Homo sapiens
5,22-cholestedien-3beta-ol desmosterol isomer, competitive inhibition Rattus norvegicus
brassicasterol DELTA22-unsaturated phytosterol, competitive inhibition Homo sapiens
brassicasterol DELTA22-unsaturated phytosterol, competitive inhibition Rattus norvegicus
ergosterol DELTA22-unsaturated phytosterol, competitive inhibition Homo sapiens
ergosterol DELTA22-unsaturated phytosterol, competitive inhibition Rattus norvegicus
additional information inhibition mechanism of DELTA22-unsaturated phytosterols Homo sapiens
additional information inhibition mechanism of DELTA22-unsaturated phytosterols Rattus norvegicus
stigmasterol DELTA22-unsaturated phytosterol, competitive inhibition Homo sapiens
stigmasterol DELTA22-unsaturated phytosterol, competitive inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
26.3
-
desmosterol pH 7.6, 37┬░C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Homo sapiens 16020
-
membrane bound Rattus norvegicus 16020
-
microsome
-
Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-cholesta-7,24-dien-3beta-ol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol 5alpha-cholest-7-en-3beta-ol + NADP+
-
?
5alpha-cholesta-7,24-dien-3beta-ol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol 5alpha-cholest-7-en-3beta-ol + NADP+
-
?
cholesta-5,7,24-trien-3beta-ol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol cholest-5,7-dien-3beta-ol + NADP+
-
?
cholesta-5,7,24-trien-3beta-ol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol cholest-5,7-dien-3beta-ol + NADP+
-
?
desmosterol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol cholesterol + NADP+
-
?
desmosterol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol cholesterol + NADP+
-
?
lanosterol + NADPH Homo sapiens step in the biosynthesis of cholesterol from lanosterol 24-dihydrolanosterol + NADP+
-
?
lanosterol + NADPH Rattus norvegicus step in the biosynthesis of cholesterol from lanosterol 24-dihydrolanosterol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell intestinal mucosa cells Homo sapiens
-
HL-60 cell promyelocytic cells Homo sapiens
-
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-cholesta-7,24-dien-3beta-ol + NADPH best substrate Homo sapiens 5alpha-cholest-7-en-3beta-ol + NADP+ i.e.lathosterol ?
5alpha-cholesta-7,24-dien-3beta-ol + NADPH best substrate Rattus norvegicus 5alpha-cholest-7-en-3beta-ol + NADP+ i.e.lathosterol ?
5alpha-cholesta-7,24-dien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens 5alpha-cholest-7-en-3beta-ol + NADP+
-
?
5alpha-cholesta-7,24-dien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus 5alpha-cholest-7-en-3beta-ol + NADP+
-
?
cholesta-5,7,24-trien-3beta-ol + NADPH
-
Homo sapiens cholest-5,7-dien-3beta-ol + NADP+
-
?
cholesta-5,7,24-trien-3beta-ol + NADPH
-
Rattus norvegicus cholest-5,7-dien-3beta-ol + NADP+
-
?
cholesta-5,7,24-trien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens cholest-5,7-dien-3beta-ol + NADP+
-
?
cholesta-5,7,24-trien-3beta-ol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus cholest-5,7-dien-3beta-ol + NADP+
-
?
desmosterol + NADPH
-
Homo sapiens cholesterol + NADP+
-
?
desmosterol + NADPH
-
Rattus norvegicus cholesterol + NADP+
-
?
desmosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens cholesterol + NADP+
-
?
desmosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus cholesterol + NADP+
-
?
lanosterol + NADPH
-
Homo sapiens 24-dihydrolanosterol + NADP+
-
?
lanosterol + NADPH
-
Rattus norvegicus 24-dihydrolanosterol + NADP+
-
?
lanosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Homo sapiens 24-dihydrolanosterol + NADP+
-
?
lanosterol + NADPH step in the biosynthesis of cholesterol from lanosterol Rattus norvegicus 24-dihydrolanosterol + NADP+
-
?
zymosterol + NADPH
-
Homo sapiens 5alpha-cholesta-8-en-3beta-ol + NADP+
-
?
zymosterol + NADPH
-
Rattus norvegicus 5alpha-cholesta-8-en-3beta-ol + NADP+
-
?

Synonyms

Synonyms Comment Organism
desmosterol reductase
-
Homo sapiens
desmosterol reductase
-
Rattus norvegicus
lanosterol reductase
-
Homo sapiens
lanosterol reductase
-
Rattus norvegicus
sterol 24DELTA-reductase
-
Homo sapiens
sterol 24DELTA-reductase
-
Rattus norvegicus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at Homo sapiens
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Homo sapiens
7.6
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0033
-
5,22-cholestedien-3beta-ol pH 7.6, 37┬░C Rattus norvegicus
0.037
-
ergosterol pH 7.6, 37┬░C Rattus norvegicus
0.041
-
stigmasterol pH 7.6, 37┬░C Rattus norvegicus
0.043
-
brassicasterol pH 7.6, 37┬░C Rattus norvegicus