Cloned (Comment) | Organism |
---|---|
gene erg6, recombinant expression in transgenic Saccharomyces cerevisiae erg6 knockout mutant strain and functional complementation | Pneumocystis carinii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | analysis of sterol compositions of wild-type Sacchromyces cerevisiae strain BY4742, the erg6 knockout mutant (DELTAerg6), and DELTAerg6 expressing the Pneumocystis carinii or the Saccharomyces cerevisiae erg6 gene by a variety of chromatographic and spectroscopic procedures. The Pneumocystis carinii SAM:SMT in the DELTAerg6 restores its ability to produce the C28 sterol ergosterol as the major sterol, and also results in low levels of C29 sterols, overview. While the Pneumocystis carinii SAM:SMT in the yeast DELTAerg6 cells is able to transfer a second methyl group to the side chain, the action of DELTA24(28)-sterol reductase (coded by the erg4 gene) in the yeast cells prevents the formation and accumulation of as many C29 sterols as that found in Pneumocystis carinii | Pneumocystis carinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+ | Pneumocystis carinii | - |
ergosterol + NADP+ | - |
? | |
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+ | Pneumocystis carinii B80 | - |
ergosterol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pneumocystis carinii | A0A0W4ZCX9 | - |
- |
Pneumocystis carinii B80 | A0A0W4ZCX9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+ | - |
Pneumocystis carinii | ergosterol + NADP+ | - |
? | |
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+ | - |
Pneumocystis carinii B80 | ergosterol + NADP+ | - |
? | |
additional information | the enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions whereas most fungal SAM:SMT transfer only one methyl group to the side chain | Pneumocystis carinii | ? | - |
? | |
additional information | the enzyme transfers either one or two methyl groups to the C-24 position of the sterol side chain producing both C28 and C29 24-alkylsterols in approximately the same proportions whereas most fungal SAM:SMT transfer only one methyl group to the side chain | Pneumocystis carinii B80 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Erg6 | - |
Pneumocystis carinii |
S-adenosylmethionine:sterol C-24 methyltransferase | - |
Pneumocystis carinii |
SAM:SMT | - |
Pneumocystis carinii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pneumocystis carinii |