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Literature summary for 1.3.1.70 extracted from
- Inhibition of sterol DELTA8 -> DELTA7-isomerase and DELTA14-reductase by fenpropimorph, tridemorph and fenpropidin in cell-free enzyme systems from Saccharomyces cerevisiae (1987), Phytochemistry, 26, 663-668.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fenpropidin | fungicide, very potent inhibitor; IC50: 0.0018 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine | Saccharomyces cerevisiae |  |
| fenpropidin | fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-piperidine | Ustilago maydis | |
| fenpropimorph | fungicide, very potent inhibitor; IC50: 0.0023 mM; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine | Saccharomyces cerevisiae | |
| fenpropimorph | fungicide, very potent inhibitor; N-[3-(p-tert-butylphenyl)-2-methylpropyl]-cis-2,6-dimethylmorpholine | Ustilago maydis | |
| tridemorph | 2,6-dimethyl-N-tridecylmorpholine; fungicide; slight inhibitor, IC50: 0.098 mM | Saccharomyces cerevisiae | |
| tridemorph | 2,6-dimethyl-N-tridecylmorpholine; fungicide | Ustilago maydis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharomyces cerevisiae | essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol | ? | - |
? | |
| additional information | Saccharomyces cerevisiae NCYC 739 | essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
NCYC 739 | - |
| Saccharomyces cerevisiae NCYC 739 | - |
NCYC 739 | - |
| Ustilago maydis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ | mechanism | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH | - |
Saccharomyces cerevisiae | 5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+ | - |
? | |
| 5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH | - |
Saccharomyces cerevisiae NCYC 739 | 5alpha-ergosta-8,24(28)-dien-3beta-ol + NADP+ | - |
? | |
| additional information | essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | essential enzyme of ergosterol, yeast sterol, biosynthesis from lanosterol | Saccharomyces cerevisiae NCYC 739 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Ustilago maydis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0018 | - |
IC50: 0.0018 mM | Saccharomyces cerevisiae | fenpropidin | |
| 0.0023 | - |
IC50: 0.0023 mM | Saccharomyces cerevisiae | fenpropimorph | |
| 0.098 | - |
slight inhibitor, IC50: 0.098 mM | Saccharomyces cerevisiae | tridemorph | |
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