General Stability | Organism |
---|---|
glycerol, 20%, stabilizes | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
trans-zeatin | high molecular weight isoenzyme | Phaseolus vulgaris | |
0.1 | 0.23 | trans-zeatin | low molecular weight isoenzyme, instability of the isoenzyme after gel filtration may contibute to the variation between experiments | Phaseolus vulgaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Phaseolus vulgaris | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
low molecular weight isoenzyme, gel filtration | Phaseolus vulgaris |
55000 | - |
high molecular weight isoenzyme, gel filtration | Phaseolus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-zeatin + NADPH | Phaseolus vulgaris | - |
dihydrozeatin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | immature | Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Phaseolus vulgaris |
Storage Stability | Organism |
---|---|
-20°C, in 0.05 M phosphate buffer containing 5 mM DTT, 0.5 mM EDTA, 5 mM NADPH, 0.5 M KCl and 20% glycerol: loss of about 10% of activity after 3 days | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with: ribosylzeatin, cis-zeatin, O-xylosylzeatin, N6-(DELTA2-isopentenyl)adenine, or N6-(DELTA2-isotentenyl)adenosine | Phaseolus vulgaris | ? | - |
? | |
trans-zeatin + NADPH | - |
Phaseolus vulgaris | dihydrozeatin + NADP+ | - |
? | |
zeatin + NADPH | - |
Phaseolus vulgaris | dihydrozeatin + NADP+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Phaseolus vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | required, NADH cannot substitute for NADPH | Phaseolus vulgaris |