Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Coprococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 83% inhibition | Coprococcus sp. | |
Co2+ | - |
Coprococcus sp. | |
Cu2+ | - |
Coprococcus sp. | |
EDTA | enzyme preincubated with the inhibitor, 0.00333 mM, for 60 min at room temperature before the addition of the substrate, 11% inhibition | Coprococcus sp. | |
Hg2+ | - |
Coprococcus sp. | |
hydroxylamine | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 9% inhibition | Coprococcus sp. | |
iodacetate | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 21% inhibition | Coprococcus sp. | |
Mg2+ | - |
Coprococcus sp. | |
Mn2+ | - |
Coprococcus sp. | |
NAD+ | with 0.33 mM, 15% inhibition | Coprococcus sp. | |
NADH | with 0.33 mM, 11% inhibition | Coprococcus sp. | |
NADP+ | with 0.33 mM, 38% inhibition | Coprococcus sp. | |
o-phenanthroline | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 71% inhibition | Coprococcus sp. | |
p-chloromercuribenzoate | enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 48% inhibition | Coprococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00003 | - |
NADPH | - |
Coprococcus sp. | |
0.00003 | - |
phloroglucinol | - |
Coprococcus sp. | |
0.00029 | - |
phloroglucinol | - |
Coprococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Coprococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phloroglucinol + NADPH | Coprococcus sp. | reverse reaction about 36fold slower then that of the forward reaction | dihydrophloroglucinol + NADP | - |
r | |
phloroglucinol + NADPH | Coprococcus sp. Pe5 | reverse reaction about 36fold slower then that of the forward reaction | dihydrophloroglucinol + NADP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprococcus sp. | - |
- |
- |
Coprococcus sp. Pe5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
protamine sulfate precipitation, ammonium sulfate precipitation, centrifugation steps, dialysis, gel filtration | Coprococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phloroglucinol + NADPH | - |
Coprococcus sp. | dihydrophloroglucinol + NADP+ | - |
r | |
phloroglucinol + NADPH | - |
Coprococcus sp. Pe5 | dihydrophloroglucinol + NADP+ | - |
r | |
phloroglucinol + NADPH | reverse reaction about 36fold slower then that of the forward reaction | Coprococcus sp. | dihydrophloroglucinol + NADP | - |
r | |
phloroglucinol + NADPH | reverse reaction about 36fold slower then that of the forward reaction | Coprococcus sp. Pe5 | dihydrophloroglucinol + NADP | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
a rapid decay of the enzyme activity in water bath at 53°C, less then 10% of the initial activity remains after 2.5 min | Coprococcus sp. |
4 | - |
crude enzyme shows a loss activity over a 5-day period, in presence of 1 mM of either 2-mercaptoethanol, dithiothreitol, or glutathione, or combinations of these reagents, is protected from the loss of enzyme activity | Coprococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Coprococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Coprococcus sp. |