BRENDA - Enzyme Database
show all sequences of 1.3.1.5

Cucurbitacin DELTA23-reductase from the fruit of Cucurbita maxima var. Green Hubbard. Physicochemical and fluorescence properties and enzyme-ligand interactions

Dirr, H.W.; Schabort, J.C.; Weitz, C.; Biochem. J. 233, 649-653 (1986)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15000
-
2 * 15000, SDS-PAGE
Cucurbita maxima
32000
-
gel filtration
Cucurbita maxima
Organism
Organism
UniProt
Commentary
Textmining
Cucurbita maxima
-
var. Green Hubbard
-
Reaction
Reaction
Commentary
Organism
Reaction ID
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
plant terpenoid
Cucurbita maxima
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Cucurbita maxima
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 15000, SDS-PAGE
Cucurbita maxima
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Cucurbita maxima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Cucurbita maxima
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15000
-
2 * 15000, SDS-PAGE
Cucurbita maxima
32000
-
gel filtration
Cucurbita maxima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Cucurbita maxima
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 15000, SDS-PAGE
Cucurbita maxima
Other publictions for EC 1.3.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390833
Dirr
Cucurbitacin DELTA23-reductase ...
Cucurbita maxima
Biochem. J.
233
649-653
1986
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390831
Schabort
Cucurbitacin B DELTA23-reducta ...
Cucurbita maxima
Biochim. Biophys. Acta
151
33-46
1968
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
1
1
1
1
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
390832
Schabort
Cucurbitacin B DELTA23-reducta ...
Cucurbita maxima
Biochim. Biophys. Acta
151
47-53
1968
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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7
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
390834
Schabort
-
The role of cucurbitacin DELTA ...
Cucurbita maxima
Phytochemistry
7
2107-2110
1968
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-
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-
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-
-
-
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-
-
-
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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-
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-
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-
-
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7
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