BRENDA - Enzyme Database
show all sequences of 1.3.1.5

Cucurbitacin B DELTA23-reductase from Cucurbita maxima. I. Assay methods, isolation and purification

Schabort, J.C.; Potgieter, D.J.J.; De Villiers, V.; Biochim. Biophys. Acta 151, 33-46 (1968)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Cucurbita maxima
-
var. Green Hubbard
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Cucurbita maxima
Reaction
Reaction
Commentary
Organism
Reaction ID
23,24-dihydrocucurbitacin B + NAD(P)+ = cucurbitacin B + NAD(P)H + H+
plant terpenoid
Cucurbita maxima
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Cucurbita maxima
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5812
-
-
Cucurbita maxima
Storage Stability
Storage Stability
Organism
0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks
Cucurbita maxima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cucurbitacin B + NAD(P)H + H+
-
390831
Cucurbita maxima
23,24-dihydrocucurbitacin B + NAD(P)+
-
-
-
ir
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Cucurbita maxima
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
5 min, no loss of activity, 10 min, 30% loss of activity
Cucurbita maxima
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.65
-
crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+
Cucurbita maxima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8
pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+
Cucurbita maxima
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Cucurbita maxima
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)+
-
Cucurbita maxima
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cucurbita maxima
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Cucurbita maxima
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5812
-
-
Cucurbita maxima
Storage Stability (protein specific)
Storage Stability
Organism
0-4°C, maleic acid-NaOH buffer, pH 6.6, 100 mM NaCl, 5 weeks
Cucurbita maxima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cucurbitacin B + NAD(P)H + H+
-
390831
Cucurbita maxima
23,24-dihydrocucurbitacin B + NAD(P)+
-
-
-
ir
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Cucurbita maxima
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
5 min, no loss of activity, 10 min, 30% loss of activity
Cucurbita maxima
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.65
-
crude enzyme preparation in presence of Zn2+ has a wider pH optimum range than purified enzyme in presence of Mn2+
Cucurbita maxima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8
pH 5.0: about 25% of activity at maximum, pH 8.0: about 80% of activity at maximum of crude enzyme preparation in presence of Zn2+, about 10% of activity at maximum of purified enzyme in presence of Mn2+
Cucurbita maxima
Other publictions for EC 1.3.1.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
390833
Dirr
Cucurbitacin DELTA23-reductase ...
Cucurbita maxima
Biochem. J.
233
649-653
1986
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-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
1
-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390831
Schabort
Cucurbitacin B DELTA23-reducta ...
Cucurbita maxima
Biochim. Biophys. Acta
151
33-46
1968
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
1
1
1
1
-
-
1
-
1
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
390832
Schabort
Cucurbitacin B DELTA23-reducta ...
Cucurbita maxima
Biochim. Biophys. Acta
151
47-53
1968
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
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-
-
-
-
-
-
-
-
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7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390834
Schabort
-
The role of cucurbitacin DELTA ...
Cucurbita maxima
Phytochemistry
7
2107-2110
1968
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-
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1
-
-
-
1
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-
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7
-
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-
-
-
-
-
-
-
1
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-
-
-
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-
1
-
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7
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