BRENDA - Enzyme Database
show all sequences of 1.3.1.48

Human prostaglandin reductase 1 (PGR1) Substrate specificity, inhibitor analysis and site-directed mutagenesis

Mesa, J.; Alsina, C.; Oppermann, U.; Pares, X.; Farres, J.; Porte, S.; Chem. Biol. Interact. 234, 105-113 (2015)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
three-dimensional structure analysis of the human enzyme, PDB ID 2Y05
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
evaluation of non-steroidal anti-inflammatory drugs (NSAIDs) as enzyme potential inhibitors
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the isozyme is zinc-independent
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
11alpha-hydroxy-9,15-dioxoprostanoate + NAD(P)+
Homo sapiens
-
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NAD(P)H + H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
P15428
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
placenta
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH + H+
-
744677
Homo sapiens
11alpha-hydroxy-9,15-dioxoprostanoate + NADP+
-
-
-
?
11alpha-hydroxy-9,15-dioxoprostanoate + NAD(P)+
-
744677
Homo sapiens
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NAD(P)H + H+
-
-
-
?
additional information
PGR1, which catalyzes the NADPH-dependent reduction of the alpha,beta-double bond of aliphatic and aromatic aldehydes and ketones, and 15-keto-prostaglandins. PGR1 also shows low activity in the oxidation of leukotriene B4. The best substrates in terms of kcat/Km are 15-keto-prostaglandins, trans-3-nonen-2-one, and trans-2-decenal. Molecular docking simulations, role of Arg56 and Tyr245 in 15-keto-prostaglandin binding, overview
744677
Homo sapiens
?
-
-
-
-
trans-2-decenal + NADPH + H+
-
744677
Homo sapiens
decanal + NADP+
-
-
-
?
trans-3-nonen-2-one + NADPH + H+
-
744677
Homo sapiens
? + NADP+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
15-keto-PG reductase
-
Homo sapiens
leukotriene B4 dehydrogenase
-
Homo sapiens
PG reductase
-
Homo sapiens
PGR1
-
Homo sapiens
prostaglandin 13-reductase
-
Homo sapiens
prostaglandin reductase 1
-
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
three-dimensional structure analysis of the human enzyme, PDB ID 2Y05
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
evaluation of non-steroidal anti-inflammatory drugs (NSAIDs) as enzyme potential inhibitors
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the isozyme is zinc-independent
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
11alpha-hydroxy-9,15-dioxoprostanoate + NAD(P)+
Homo sapiens
-
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NAD(P)H + H+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
placenta
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NADPH + H+
-
744677
Homo sapiens
11alpha-hydroxy-9,15-dioxoprostanoate + NADP+
-
-
-
?
11alpha-hydroxy-9,15-dioxoprostanoate + NAD(P)+
-
744677
Homo sapiens
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate + NAD(P)H + H+
-
-
-
?
additional information
PGR1, which catalyzes the NADPH-dependent reduction of the alpha,beta-double bond of aliphatic and aromatic aldehydes and ketones, and 15-keto-prostaglandins. PGR1 also shows low activity in the oxidation of leukotriene B4. The best substrates in terms of kcat/Km are 15-keto-prostaglandins, trans-3-nonen-2-one, and trans-2-decenal. Molecular docking simulations, role of Arg56 and Tyr245 in 15-keto-prostaglandin binding, overview
744677
Homo sapiens
?
-
-
-
-
trans-2-decenal + NADPH + H+
-
744677
Homo sapiens
decanal + NADP+
-
-
-
?
trans-3-nonen-2-one + NADPH + H+
-
744677
Homo sapiens
? + NADP+
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the three human PG reductases are zinc-independent members of the medium-chain dehydrogenase/reductase (MDR) superfamily
Homo sapiens
metabolism
prostaglandins are lipid compounds derived from arachidonic acid by the action of cyclooxygenases, acting locally as messenger molecules in a wide variety of physiological processes, such as inflammation, cell survival, apoptosis, smooth muscle contraction, adipocyte differentiation, vasodilation and platelet aggregation inhibition. In the inactivating pathway of prostaglandins, the first metabolic intermediates are 15-keto-prostaglandins, which are further converted into 13,14-dihydro-15-keto-prostaglandins by different enzymes having 15-keto-prostaglandin reductase activity
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
evolution
the three human PG reductases are zinc-independent members of the medium-chain dehydrogenase/reductase (MDR) superfamily
Homo sapiens
metabolism
prostaglandins are lipid compounds derived from arachidonic acid by the action of cyclooxygenases, acting locally as messenger molecules in a wide variety of physiological processes, such as inflammation, cell survival, apoptosis, smooth muscle contraction, adipocyte differentiation, vasodilation and platelet aggregation inhibition. In the inactivating pathway of prostaglandins, the first metabolic intermediates are 15-keto-prostaglandins, which are further converted into 13,14-dihydro-15-keto-prostaglandins by different enzymes having 15-keto-prostaglandin reductase activity
Homo sapiens
Other publictions for EC 1.3.1.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744677
Mesa
Human prostaglandin reductase ...
Homo sapiens
Chem. Biol. Interact.
234
105-113
2015
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2
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746356
Piao
Wound healing effects of new ...
Homo sapiens
Prostaglandins Leukot. Essent. Fatty Acids
91
325-332
2014
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1
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26
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1
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1
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1
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26
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1
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699958
Zhao
Restoration of leukotriene B(4 ...
Sus scrofa
Lung Cancer
68
161-169
2010
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1
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690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
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1
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6
9
1
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1
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1
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11
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3
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1
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1
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11
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687562
Chou
Identification of a novel pros ...
Mus musculus
J. Biol. Chem.
282
18162-18172
2007
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1
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5
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1
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1
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4
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5
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4
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5
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5
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674369
Hori
Crystal structure of anti-conf ...
Cavia porcellus
J. Biochem.
140
457-466
2006
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1
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1
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656219
Hori
Structural basis of leukotrien ...
Cavia porcellus
J. Biol. Chem.
279
22615-22623
2004
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1
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1
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1
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390829
Yamamoto
Immunohistochemical localizati ...
Cavia porcellus
Eur. J. Biochem.
268
6105-6113
2001
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1
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2
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8
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1
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1
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2
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390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
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1
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20
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3
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1
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20
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1
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20
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20
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1
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20
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20
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390828
Clish
Oxidoreductases in lipoxin A4 ...
Sus scrofa
J. Biol. Chem.
275
25372-25380
2000
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1
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3
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1
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390827
Ensor
-
Purification, cDNA cloning and ...
Bos taurus, Sus scrofa
Biochem. J.
330
103-108
1998
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1
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10
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7
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2
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390825
Nikaido
15-Ketoprostaglandin delta 13- ...
Bos taurus
Curr. Eye Res.
12
17-22
1993
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4
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1
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2
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390826
Kitamura
Purification of a 15-ketoprost ...
Rattus norvegicus
Biochem. Mol. Biol. Int.
30
839-847
1993
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4
1
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2
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1
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10
1
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1
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1
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22
1
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1
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390820
Hansen
Purification and assay of 15-k ...
Bos taurus, Gallus gallus, Homo sapiens
Methods Enzymol.
86
156-167
1982
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3
7
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2
6
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3
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1
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3
1
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15
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1
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15
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1
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390821
Jarabak
Isolation and properties of a ...
Homo sapiens
Methods Enzymol.
86
163-167
1982
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3
7
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1
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7
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1
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7
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1
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390823
Hansen
Purification and characterizat ...
Bos taurus
Biochim. Biophys. Acta
574
136-145
1979
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2
2
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1
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1
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1
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1
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390824
Westbrook
15-Ketoprostaglandin delta13 r ...
Homo sapiens
Arch. Biochem. Biophys.
185
429-442
1978
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5
7
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2
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1
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1
1
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8
1
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1
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2
3
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2
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5
3
7
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2
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1
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1
1
1
8
1
1
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390822
Westbrook
Purification and partial chara ...
Homo sapiens
Biochem. Biophys. Res. Commun.
66
541-546
1975
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2
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2
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