BRENDA - Enzyme Database
show all sequences of 1.3.1.48

Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin DELTA13-reductase (LTB4 12-HD/PGR) responsible for the reduction of a double-bond of the alpha,beta-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670

Itoh, K.; Yamamoto, K.; Adachi, M.; Kosaka, T.; Tanaka, Y.; Xenobiotica 38, 249-263 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli Top10 cells
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
15-keto prostaglandin F2alpha
43.06% residual activity at 0.1 mM
Rattus norvegicus
benzylidene acetophenone
21.09% residual activity at 0.5 mM
Rattus norvegicus
dicoumarol
11.45% residual activity at 0.1 mM
Rattus norvegicus
indomethacin
14.22% residual activity at 0.1 mM
Rattus norvegicus
additional information
finasteride has no inhibitory effect
Rattus norvegicus
quercitrin
13.14% residual activity at 0.1 mM
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0078
-
15-ketoprostaglandin E2
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.0123
-
phenyl propenyl ketone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.0277
-
benzylidene acetone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.0791
-
cinnamoylthiophene
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.1289
-
Methyl vinyl ketone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.1399
-
benzylidene acetonphenone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.461
-
4-hydroxy-2-nonenal
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
1.426
-
cinnamaldehyde
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
2.194
-
trans-2-hexenal
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Organism
Organism
UniProt
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
2',5'-ADP Sepharose 4B column chromatography and HisTrap HP column chromatography
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NADPH
-
690204
Rattus norvegicus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NADP+
-
-
-
?
15-ketoprostaglandin E2 + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
4-hydroxy-2-nonenal + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
benzylidene acetone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
benzylidene acetonphenone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
cinnamaldehyde + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
cinnamoylthiophene + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
leukotriene B4 + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
methyl vinyl ketone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
phenyl propenyl ketone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
trans-2-hexenal + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
15-ketoprostaglandinDELTA13-reductase
-
Rattus norvegicus
bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase
Rattus norvegicus
PGR
-
Rattus norvegicus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Top10 cells
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
15-keto prostaglandin F2alpha
43.06% residual activity at 0.1 mM
Rattus norvegicus
benzylidene acetophenone
21.09% residual activity at 0.5 mM
Rattus norvegicus
dicoumarol
11.45% residual activity at 0.1 mM
Rattus norvegicus
indomethacin
14.22% residual activity at 0.1 mM
Rattus norvegicus
additional information
finasteride has no inhibitory effect
Rattus norvegicus
quercitrin
13.14% residual activity at 0.1 mM
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0078
-
15-ketoprostaglandin E2
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.0123
-
phenyl propenyl ketone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.0277
-
benzylidene acetone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.0791
-
cinnamoylthiophene
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.1289
-
Methyl vinyl ketone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.1399
-
benzylidene acetonphenone
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
0.461
-
4-hydroxy-2-nonenal
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
1.426
-
cinnamaldehyde
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
2.194
-
trans-2-hexenal
in 100 mM phosphate buffer (pH 7.4), at 25°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Rattus norvegicus
5829
-
Purification (Commentary) (protein specific)
Commentary
Organism
2',5'-ADP Sepharose 4B column chromatography and HisTrap HP column chromatography
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NADPH
-
690204
Rattus norvegicus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NADP+
-
-
-
?
15-ketoprostaglandin E2 + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
4-hydroxy-2-nonenal + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
benzylidene acetone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
benzylidene acetonphenone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
cinnamaldehyde + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
cinnamoylthiophene + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
leukotriene B4 + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
methyl vinyl ketone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
phenyl propenyl ketone + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
trans-2-hexenal + NADPH
-
690204
Rattus norvegicus
?
-
-
-
?
Other publictions for EC 1.3.1.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744677
Mesa
Human prostaglandin reductase ...
Homo sapiens
Chem. Biol. Interact.
234
105-113
2015
-
-
-
1
-
-
1
-
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1
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1
-
2
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1
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5
-
6
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1
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1
1
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1
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1
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1
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1
-
-
5
-
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-
-
-
-
-
-
-
-
2
2
-
-
-
746356
Piao
Wound healing effects of new ...
Homo sapiens
Prostaglandins Leukot. Essent. Fatty Acids
91
325-332
2014
-
-
1
-
-
-
26
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
2
1
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-
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1
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1
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22
-
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1
1
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22
26
-
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1
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1
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2
-
-
1
-
1
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-
-
1
-
-
-
-
1
1
-
-
-
699958
Zhao
Restoration of leukotriene B(4 ...
Sus scrofa
Lung Cancer
68
161-169
2010
-
-
1
-
-
-
-
-
-
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1
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2
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1
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1
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4
1
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1
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1
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1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
-
-
1
-
-
-
6
9
1
-
-
-
-
1
-
-
1
-
-
1
-
-
11
-
3
-
-
-
-
-
-
-
1
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-
-
-
-
1
1
-
-
-
-
6
-
9
1
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-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687562
Chou
Identification of a novel pros ...
Mus musculus
J. Biol. Chem.
282
18162-18172
2007
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
2
4
-
5
-
2
-
-
-
5
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
2
4
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
674369
Hori
Crystal structure of anti-conf ...
Cavia porcellus
J. Biochem.
140
457-466
2006
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656219
Hori
Structural basis of leukotrien ...
Cavia porcellus
J. Biol. Chem.
279
22615-22623
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
2
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-
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-
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-
1
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390829
Yamamoto
Immunohistochemical localizati ...
Cavia porcellus
Eur. J. Biochem.
268
6105-6113
2001
-
-
1
-
-
-
-
2
-
-
-
-
-
8
-
-
1
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
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1
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2
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1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
-
20
-
-
-
-
-
3
-
-
1
-
-
-
-
-
20
-
1
-
-
-
20
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
1
-
-
-
-
20
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
390828
Clish
Oxidoreductases in lipoxin A4 ...
Sus scrofa
J. Biol. Chem.
275
25372-25380
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
1
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1
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1
1
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-
1
-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
390827
Ensor
-
Purification, cDNA cloning and ...
Bos taurus, Sus scrofa
Biochem. J.
330
103-108
1998
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
2
2
-
7
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
2
2
-
7
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
390825
Nikaido
15-Ketoprostaglandin delta 13- ...
Bos taurus
Curr. Eye Res.
12
17-22
1993
-
-
-
-
-
-
4
-
1
-
2
-
-
1
-
-
1
-
-
4
6
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
2
-
-
-
-
1
-
4
6
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390826
Kitamura
Purification of a 15-ketoprost ...
Rattus norvegicus
Biochem. Mol. Biol. Int.
30
839-847
1993
-
-
-
-
-
-
4
1
-
-
2
-
-
1
-
-
1
-
-
10
1
-
22
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
10
1
-
22
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
390820
Hansen
Purification and assay of 15-k ...
Bos taurus, Gallus gallus, Homo sapiens
Methods Enzymol.
86
156-167
1982
-
-
-
-
-
-
3
7
-
-
2
6
-
3
-
-
1
-
-
3
1
2
15
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
7
-
-
2
6
-
-
-
1
-
3
1
2
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390821
Jarabak
Isolation and properties of a ...
Homo sapiens
Methods Enzymol.
86
163-167
1982
-
-
-
-
-
-
3
7
-
-
-
-
-
1
-
-
1
-
-
1
1
2
7
-
-
1
-
-
-
-
-
-
3
2
-
-
-
-
-
3
-
-
-
-
3
2
7
-
-
-
-
-
-
-
1
-
1
1
2
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390823
Hansen
Purification and characterizat ...
Bos taurus
Biochim. Biophys. Acta
574
136-145
1979
-
-
-
-
-
-
2
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
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2
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1
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1
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1
1
1
2
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1
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1
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390824
Westbrook
15-Ketoprostaglandin delta13 r ...
Homo sapiens
Arch. Biochem. Biophys.
185
429-442
1978
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5
7
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2
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1
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1
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1
1
1
8
1
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1
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2
3
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2
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5
3
7
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2
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1
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1
1
1
8
1
1
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390822
Westbrook
Purification and partial chara ...
Homo sapiens
Biochem. Biophys. Res. Commun.
66
541-546
1975
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2
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2
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1
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1
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1
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1
1
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1
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1
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1
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1
1
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