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Literature summary for 1.3.1.48 extracted from

  • Itoh, K.; Yamamoto, K.; Adachi, M.; Kosaka, T.; Tanaka, Y.
    Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin DELTA13-reductase (LTB4 12-HD/PGR) responsible for the reduction of a double-bond of the alpha,beta-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670 (2008), Xenobiotica, 38, 249-263.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
15-keto prostaglandin F2alpha 43.06% residual activity at 0.1 mM Rattus norvegicus
benzylidene acetophenone 21.09% residual activity at 0.5 mM Rattus norvegicus
dicoumarol 11.45% residual activity at 0.1 mM Rattus norvegicus
indomethacin 14.22% residual activity at 0.1 mM Rattus norvegicus
additional information finasteride has no inhibitory effect Rattus norvegicus
quercitrin 13.14% residual activity at 0.1 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
15-ketoprostaglandin E2 in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
0.0123
-
phenyl propenyl ketone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
0.0277
-
benzylidene acetone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
0.0791
-
cinnamoylthiophene in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
0.1289
-
Methyl vinyl ketone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
0.1399
-
benzylidene acetonphenone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
0.461
-
4-hydroxy-2-nonenal in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.426
-
cinnamaldehyde in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
2.194
-
trans-2-hexenal in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP Sepharose 4B column chromatography and HisTrap HP column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NADPH
-
Rattus norvegicus 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NADP+
-
?
15-ketoprostaglandin E2 + NADPH
-
Rattus norvegicus ?
-
?
4-hydroxy-2-nonenal + NADPH
-
Rattus norvegicus ?
-
?
benzylidene acetone + NADPH
-
Rattus norvegicus ?
-
?
benzylidene acetonphenone + NADPH
-
Rattus norvegicus ?
-
?
cinnamaldehyde + NADPH
-
Rattus norvegicus ?
-
?
cinnamoylthiophene + NADPH
-
Rattus norvegicus ?
-
?
leukotriene B4 + NADPH
-
Rattus norvegicus ?
-
?
methyl vinyl ketone + NADPH
-
Rattus norvegicus ?
-
?
phenyl propenyl ketone + NADPH
-
Rattus norvegicus ?
-
?
trans-2-hexenal + NADPH
-
Rattus norvegicus ?
-
?

Synonyms

Synonyms Comment Organism
15-ketoprostaglandinDELTA13-reductase
-
Rattus norvegicus
bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase Rattus norvegicus
PGR
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Rattus norvegicus