BRENDA - Enzyme Database
show all sequences of 1.3.1.48

Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation

Chou, W.L.; Chuang, L.M.; Chou, C.C.; Wang, A.H.; Lawson, J.A.; FitzGerald, G.A.; Chang, Z.F.; J. Biol. Chem. 282, 18162-18172 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli strain XL1-Blue
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0344
-
15-ketoprostaglandin E1
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.0496
-
15-ketoprostaglandin E2
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.0592
-
15-ketoprostaglandin F1alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.0946
-
NADPH
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.1088
-
15-ketoprostaglandin F2alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
Organism
Organism
UniProt
Commentary
Textmining
Mus musculus
Q8VDQ1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
glutathione-Sepharose column chromatography
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
3T3-L1 cell
-
Mus musculus
-
adipocyte
-
Mus musculus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.115
-
using 15-ketoprostaglandin E1 as a substrate, at 37C
Mus musculus
0.1784
-
using 15-ketoprostaglandin E2 as a substrate, at 37C
Mus musculus
0.2064
-
using 15-ketoprostaglandin F1alpha as a substrate, at 37C
Mus musculus
0.2309
-
using 15-ketoprostaglandin F2alpha as a substrate, at 37C
Mus musculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
15-ketoprostaglandin E1 + NADPH
-
687562
Mus musculus
?
-
-
-
?
15-ketoprostaglandin E2 + NADPH
-
687562
Mus musculus
13,14-dihydro-15-keto-prostaglandin E2 + NADP+
-
-
-
?
15-ketoprostaglandin F1alpha + NADPH
-
687562
Mus musculus
?
-
-
-
?
15-ketoprostaglandin F2alpha + NADPH
-
687562
Mus musculus
?
-
-
-
?
additional information
not active towards 6-keto-prostaglandin F1alpha, prostaglandin F2beta, 11beta-prostaglandin F2alpha, 13,14-dihydro-15-keto-prostaglandin D2, 13,14-dihydro-15-keto-prostaglandin E2, 13,14-dihydro-15-keto-PGF2alpha, and leukotriene B4
687562
Mus musculus
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
15-oxoprostaglandin-DELTA13-reductase
-
Mus musculus
PGR-2
-
Mus musculus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.12
-
15-ketoprostaglandin E1
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.16
-
NADPH
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.19
-
15-ketoprostaglandin E2
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.22
-
15-ketoprostaglandin F1alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.25
-
15-ketoprostaglandin F2alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli strain XL1-Blue
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0344
-
15-ketoprostaglandin E1
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.0496
-
15-ketoprostaglandin E2
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.0592
-
15-ketoprostaglandin F1alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.0946
-
NADPH
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.1088
-
15-ketoprostaglandin F2alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione-Sepharose column chromatography
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
3T3-L1 cell
-
Mus musculus
-
adipocyte
-
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.115
-
using 15-ketoprostaglandin E1 as a substrate, at 37C
Mus musculus
0.1784
-
using 15-ketoprostaglandin E2 as a substrate, at 37C
Mus musculus
0.2064
-
using 15-ketoprostaglandin F1alpha as a substrate, at 37C
Mus musculus
0.2309
-
using 15-ketoprostaglandin F2alpha as a substrate, at 37C
Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
15-ketoprostaglandin E1 + NADPH
-
687562
Mus musculus
?
-
-
-
?
15-ketoprostaglandin E2 + NADPH
-
687562
Mus musculus
13,14-dihydro-15-keto-prostaglandin E2 + NADP+
-
-
-
?
15-ketoprostaglandin F1alpha + NADPH
-
687562
Mus musculus
?
-
-
-
?
15-ketoprostaglandin F2alpha + NADPH
-
687562
Mus musculus
?
-
-
-
?
additional information
not active towards 6-keto-prostaglandin F1alpha, prostaglandin F2beta, 11beta-prostaglandin F2alpha, 13,14-dihydro-15-keto-prostaglandin D2, 13,14-dihydro-15-keto-prostaglandin E2, 13,14-dihydro-15-keto-PGF2alpha, and leukotriene B4
687562
Mus musculus
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.12
-
15-ketoprostaglandin E1
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.16
-
NADPH
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.19
-
15-ketoprostaglandin E2
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.22
-
15-ketoprostaglandin F1alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
0.25
-
15-ketoprostaglandin F2alpha
in 0.1 M Tris-HCl (pH 7.4), at 37C
Mus musculus
Other publictions for EC 1.3.1.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744677
Mesa
Human prostaglandin reductase ...
Homo sapiens
Chem. Biol. Interact.
234
105-113
2015
-
-
-
1
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
1
-
-
5
-
6
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
746356
Piao
Wound healing effects of new ...
Homo sapiens
Prostaglandins Leukot. Essent. Fatty Acids
91
325-332
2014
-
-
1
-
-
-
26
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
2
1
-
-
-
1
-
-
1
-
-
22
-
-
1
1
-
-
-
22
26
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
699958
Zhao
Restoration of leukotriene B(4 ...
Sus scrofa
Lung Cancer
68
161-169
2010
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
1
-
1
-
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
-
-
1
-
-
-
6
9
1
-
-
-
-
1
-
-
1
-
-
1
-
-
11
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
9
1
-
-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687562
Chou
Identification of a novel pros ...
Mus musculus
J. Biol. Chem.
282
18162-18172
2007
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
2
4
-
5
-
2
-
-
-
5
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
2
4
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
674369
Hori
Crystal structure of anti-conf ...
Cavia porcellus
J. Biochem.
140
457-466
2006
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656219
Hori
Structural basis of leukotrien ...
Cavia porcellus
J. Biol. Chem.
279
22615-22623
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390829
Yamamoto
Immunohistochemical localizati ...
Cavia porcellus
Eur. J. Biochem.
268
6105-6113
2001
-
-
1
-
-
-
-
2
-
-
-
-
-
8
-
-
1
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
-
20
-
-
-
-
-
3
-
-
1
-
-
-
-
-
20
-
1
-
-
-
20
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
1
-
-
-
-
20
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
390828
Clish
Oxidoreductases in lipoxin A4 ...
Sus scrofa
J. Biol. Chem.
275
25372-25380
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390827
Ensor
-
Purification, cDNA cloning and ...
Bos taurus, Sus scrofa
Biochem. J.
330
103-108
1998
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
2
2
-
7
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
2
2
-
7
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
390825
Nikaido
15-Ketoprostaglandin delta 13- ...
Bos taurus
Curr. Eye Res.
12
17-22
1993
-
-
-
-
-
-
4
-
1
-
2
-
-
1
-
-
1
-
-
4
6
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
2
-
-
-
-
1
-
4
6
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390826
Kitamura
Purification of a 15-ketoprost ...
Rattus norvegicus
Biochem. Mol. Biol. Int.
30
839-847
1993
-
-
-
-
-
-
4
1
-
-
2
-
-
1
-
-
1
-
-
10
1
-
22
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
10
1
-
22
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
390820
Hansen
Purification and assay of 15-k ...
Bos taurus, Gallus gallus, Homo sapiens
Methods Enzymol.
86
156-167
1982
-
-
-
-
-
-
3
7
-
-
2
6
-
3
-
-
1
-
-
3
1
2
15
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
7
-
-
2
6
-
-
-
1
-
3
1
2
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390821
Jarabak
Isolation and properties of a ...
Homo sapiens
Methods Enzymol.
86
163-167
1982
-
-
-
-
-
-
3
7
-
-
-
-
-
1
-
-
1
-
-
1
1
2
7
-
-
1
-
-
-
-
-
-
3
2
-
-
-
-
-
3
-
-
-
-
3
2
7
-
-
-
-
-
-
-
1
-
1
1
2
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390823
Hansen
Purification and characterizat ...
Bos taurus
Biochim. Biophys. Acta
574
136-145
1979
-
-
-
-
-
-
2
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
1
1
1
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
390824
Westbrook
15-Ketoprostaglandin delta13 r ...
Homo sapiens
Arch. Biochem. Biophys.
185
429-442
1978
-
-
-
-
-
-
5
7
-
-
2
-
-
1
-
-
1
-
-
1
1
1
8
1
-
1
-
-
-
-
-
-
2
3
-
-
-
-
-
2
-
-
-
-
5
3
7
-
-
2
-
-
-
-
1
-
1
1
1
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390822
Westbrook
Purification and partial chara ...
Homo sapiens
Biochem. Biophys. Res. Commun.
66
541-546
1975
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
2
1
-
3
-
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
2
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-