BRENDA - Enzyme Database
show all sequences of 1.3.1.48

Purification, cDNA cloning and expression of 15-oxoprostaglandin 13-reductase from pig lung

Ensor, C.M.; Zhang, H.; Tai, H.H.; Biochem. J. 330, 103-108 (1998)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Sus scrofa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
15-ketoprostaglandin E1
-
Sus scrofa
0.0083
-
15-ketoprostaglandin E1
recombinant enzyme
Sus scrofa
0.01
-
leukotriene B4
recombinant enzyme
Sus scrofa
0.015
-
NADPH
-
Sus scrofa
0.016
-
NADPH
recombinant enzyme
Sus scrofa
0.0182
-
15-ketoprostaglandin E2
recombinant enzyme
Sus scrofa
0.019
-
15-ketoprostaglandin E2
-
Sus scrofa
0.088
0.094
NADH
-
Bos taurus
0.153
-
NADH
-
Sus scrofa
0.16
-
NADH
recombinant enzyme
Sus scrofa
Organism
Organism
UniProt
Commentary
Textmining
Bos taurus
-
-
-
Sus scrofa
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native and recombinant enzyme
Sus scrofa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
lung
-
Bos taurus
-
lung
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.56
-
-
Sus scrofa
2.6
-
recombinant enzyme expressed in Escherichia coli
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(5S,12R)-dihydroxy-(6E,8Z,10Z,14E)-eicosatetraenoic acid + NADH
trivial name leukotriene B4, enzyme exhibits a 15-ketoprostaglandin-13-reductase and a very low leukotriene B4 12-hydroxydehydrogenase activity
390827
Sus scrofa
?
-
-
-
?
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
390827
Sus scrofa
(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
390827
Sus scrofa
ir
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
390827
Bos taurus
(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
390827
Bos taurus
ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390827
Sus scrofa
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
-
-
?
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390827
Bos taurus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
390827
Bos taurus
?
15-ketoprostaglandin E1 + NADPH
-
390827
Sus scrofa
?
-
-
-
-
leukotriene B4 + NADPH
-
390827
Sus scrofa
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.517
-
15-ketoprostaglandin E1
cofactor NADH
Sus scrofa
1.52
-
15-ketoprostaglandin E1
cofactor NADPH
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Bos taurus
NAD(P)H
3fold higher activity with NADPH compared to NADH
Sus scrofa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Bos taurus
NAD(P)H
3fold higher activity with NADPH compared to NADH
Sus scrofa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0077
-
15-ketoprostaglandin E1
-
Sus scrofa
0.0083
-
15-ketoprostaglandin E1
recombinant enzyme
Sus scrofa
0.01
-
leukotriene B4
recombinant enzyme
Sus scrofa
0.015
-
NADPH
-
Sus scrofa
0.016
-
NADPH
recombinant enzyme
Sus scrofa
0.0182
-
15-ketoprostaglandin E2
recombinant enzyme
Sus scrofa
0.019
-
15-ketoprostaglandin E2
-
Sus scrofa
0.088
0.094
NADH
-
Bos taurus
0.153
-
NADH
-
Sus scrofa
0.16
-
NADH
recombinant enzyme
Sus scrofa
Purification (Commentary) (protein specific)
Commentary
Organism
native and recombinant enzyme
Sus scrofa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
lung
-
Bos taurus
-
lung
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.56
-
-
Sus scrofa
2.6
-
recombinant enzyme expressed in Escherichia coli
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(5S,12R)-dihydroxy-(6E,8Z,10Z,14E)-eicosatetraenoic acid + NADH
trivial name leukotriene B4, enzyme exhibits a 15-ketoprostaglandin-13-reductase and a very low leukotriene B4 12-hydroxydehydrogenase activity
390827
Sus scrofa
?
-
-
-
?
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
390827
Sus scrofa
(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
390827
Sus scrofa
ir
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
390827
Bos taurus
(5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
390827
Bos taurus
ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390827
Sus scrofa
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
-
-
?
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390827
Bos taurus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
390827
Bos taurus
?
15-ketoprostaglandin E1 + NADPH
-
390827
Sus scrofa
?
-
-
-
-
leukotriene B4 + NADPH
-
390827
Sus scrofa
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.517
-
15-ketoprostaglandin E1
cofactor NADH
Sus scrofa
1.52
-
15-ketoprostaglandin E1
cofactor NADPH
Sus scrofa
Other publictions for EC 1.3.1.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744677
Mesa
Human prostaglandin reductase ...
Homo sapiens
Chem. Biol. Interact.
234
105-113
2015
-
-
-
1
-
-
1
-
-
1
-
1
-
2
-
-
-
-
-
1
-
-
5
-
6
-
-
-
-
-
-
-
1
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-
-
-
-
-
1
1
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
746356
Piao
Wound healing effects of new ...
Homo sapiens
Prostaglandins Leukot. Essent. Fatty Acids
91
325-332
2014
-
-
1
-
-
-
26
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
2
1
-
-
-
1
-
-
1
-
-
22
-
-
1
1
-
-
-
22
26
-
-
1
-
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1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
699958
Zhao
Restoration of leukotriene B(4 ...
Sus scrofa
Lung Cancer
68
161-169
2010
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
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-
-
1
-
1
-
4
1
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1
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1
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1
1
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1
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1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
-
-
1
-
-
-
6
9
1
-
-
-
-
1
-
-
1
-
-
1
-
-
11
-
3
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1
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1
1
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6
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9
1
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1
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1
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11
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-
687562
Chou
Identification of a novel pros ...
Mus musculus
J. Biol. Chem.
282
18162-18172
2007
-
-
1
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-
5
-
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1
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1
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2
4
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5
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2
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5
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1
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1
1
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5
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-
1
-
2
4
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
674369
Hori
Crystal structure of anti-conf ...
Cavia porcellus
J. Biochem.
140
457-466
2006
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656219
Hori
Structural basis of leukotrien ...
Cavia porcellus
J. Biol. Chem.
279
22615-22623
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
1
-
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-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390829
Yamamoto
Immunohistochemical localizati ...
Cavia porcellus
Eur. J. Biochem.
268
6105-6113
2001
-
-
1
-
-
-
-
2
-
-
-
-
-
8
-
-
1
-
-
1
-
-
2
-
1
-
-
-
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-
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1
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2
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1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
-
20
-
-
-
-
-
3
-
-
1
-
-
-
-
-
20
-
1
-
-
-
20
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
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-
20
-
-
-
-
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-
-
1
-
-
-
-
20
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
390828
Clish
Oxidoreductases in lipoxin A4 ...
Sus scrofa
J. Biol. Chem.
275
25372-25380
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
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-
-
1
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-
1
-
1
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-
1
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1
1
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1
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1
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-
390827
Ensor
-
Purification, cDNA cloning and ...
Bos taurus, Sus scrofa
Biochem. J.
330
103-108
1998
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
2
2
-
7
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
2
2
-
7
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
390825
Nikaido
15-Ketoprostaglandin delta 13- ...
Bos taurus
Curr. Eye Res.
12
17-22
1993
-
-
-
-
-
-
4
-
1
-
2
-
-
1
-
-
1
-
-
4
6
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
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4
-
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1
-
2
-
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1
-
4
6
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390826
Kitamura
Purification of a 15-ketoprost ...
Rattus norvegicus
Biochem. Mol. Biol. Int.
30
839-847
1993
-
-
-
-
-
-
4
1
-
-
2
-
-
1
-
-
1
-
-
10
1
-
22
1
-
-
-
-
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1
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1
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1
-
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4
-
1
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2
-
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1
-
10
1
-
22
1
-
-
-
-
-
1
-
-
-
-
-
-
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-
390820
Hansen
Purification and assay of 15-k ...
Bos taurus, Gallus gallus, Homo sapiens
Methods Enzymol.
86
156-167
1982
-
-
-
-
-
-
3
7
-
-
2
6
-
3
-
-
1
-
-
3
1
2
15
-
-
1
-
-
-
-
-
-
3
-
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-
-
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-
3
-
-
-
-
3
-
7
-
-
2
6
-
-
-
1
-
3
1
2
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390821
Jarabak
Isolation and properties of a ...
Homo sapiens
Methods Enzymol.
86
163-167
1982
-
-
-
-
-
-
3
7
-
-
-
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-
1
-
-
1
-
-
1
1
2
7
-
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1
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3
2
-
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-
-
3
-
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-
3
2
7
-
-
-
-
-
-
-
1
-
1
1
2
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390823
Hansen
Purification and characterizat ...
Bos taurus
Biochim. Biophys. Acta
574
136-145
1979
-
-
-
-
-
-
2
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
2
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
1
1
1
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
390824
Westbrook
15-Ketoprostaglandin delta13 r ...
Homo sapiens
Arch. Biochem. Biophys.
185
429-442
1978
-
-
-
-
-
-
5
7
-
-
2
-
-
1
-
-
1
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-
1
1
1
8
1
-
1
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-
-
-
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2
3
-
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-
-
2
-
-
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-
5
3
7
-
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2
-
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-
-
1
-
1
1
1
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390822
Westbrook
Purification and partial chara ...
Homo sapiens
Biochem. Biophys. Res. Commun.
66
541-546
1975
-
-
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2
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2
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1
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1
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3
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1
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1
1
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2
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2
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2
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1
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2
1
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3
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1
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