BRENDA - Enzyme Database show
show all sequences of 1.3.1.48

15-Ketoprostaglandin delta 13-reductase activity in bovine ocular tissues

Nikaido, H.; Moriishi, M.; Mishima, H.K.; Kitamura, S.; Tatsumi, K.; Curr. Eye Res. 12, 17-22 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
dicoumarol
0.1 mM, 86% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
Disulfiram
0.1 mM, 61% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
indomethacin
0.1 mM, 97% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
quercitrin
0.1 mM, 76% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
gel filtration
Bos taurus
57000
-
1 * 57000, SDS-PAGE
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
ciliary body
-
Bos taurus
-
cornea
-
Bos taurus
-
iris
-
Bos taurus
-
retina
retinal pigment epithelium choroid
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00026
-
enzyme activity in cornea, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.000389
-
enzyme activity in retina, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.00085
-
enzyme activity in retinal pigment epithelium, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.000945
-
enzyme purified from iris-ciliary cytosol
Bos taurus
0.0012
-
enzyme activity in ciliary body, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.00145
-
enzyme activity in iris, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
Storage Stability
Storage Stability
Organism
-80°C, NADH-linked activity is lost overnight
Bos taurus
0°C, NADPH-linked activity is lost after a few hours
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390825
Bos taurus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
390825
Bos taurus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 57000, SDS-PAGE
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
enzyme from iris-ciliary body prefers NADPH
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
enzyme from iris-ciliary body prefers NADPH
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dicoumarol
0.1 mM, 86% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
Disulfiram
0.1 mM, 61% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
indomethacin
0.1 mM, 97% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
quercitrin
0.1 mM, 76% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
gel filtration
Bos taurus
57000
-
1 * 57000, SDS-PAGE
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
ciliary body
-
Bos taurus
-
cornea
-
Bos taurus
-
iris
-
Bos taurus
-
retina
retinal pigment epithelium choroid
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00026
-
enzyme activity in cornea, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.000389
-
enzyme activity in retina, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.00085
-
enzyme activity in retinal pigment epithelium, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.000945
-
enzyme purified from iris-ciliary cytosol
Bos taurus
0.0012
-
enzyme activity in ciliary body, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
0.00145
-
enzyme activity in iris, substrate 15-ketoprostaglandin, cofactor NADPH
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, NADH-linked activity is lost overnight
Bos taurus
0°C, NADPH-linked activity is lost after a few hours
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
390825
Bos taurus
11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
390825
Bos taurus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 57000, SDS-PAGE
Bos taurus
Other publictions for EC 1.3.1.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744677
Mesa
Human prostaglandin reductase ...
Homo sapiens
Chem. Biol. Interact.
234
105-113
2015
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1
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1
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1
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1
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2
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1
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5
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1
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1
1
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1
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1
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1
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1
-
-
5
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
746356
Piao
Wound healing effects of new ...
Homo sapiens
Prostaglandins Leukot. Essent. Fatty Acids
91
325-332
2014
-
-
1
-
-
-
26
-
1
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
1
-
-
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1
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1
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22
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1
1
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22
26
-
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1
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1
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2
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1
-
1
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1
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1
1
-
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-
699958
Zhao
Restoration of leukotriene B(4 ...
Sus scrofa
Lung Cancer
68
161-169
2010
-
-
1
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1
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2
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1
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1
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1
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1
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1
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1
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1
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-
690204
Itoh
Leukotriene B4 12-hydroxydehyd ...
Rattus norvegicus
Xenobiotica
38
249-263
2008
-
-
1
-
-
-
6
9
1
-
-
-
-
1
-
-
1
-
-
1
-
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
9
1
-
-
-
-
-
-
1
-
1
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687562
Chou
Identification of a novel pros ...
Mus musculus
J. Biol. Chem.
282
18162-18172
2007
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
1
-
-
2
4
-
5
-
-
-
-
5
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
2
4
-
5
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
674369
Hori
Crystal structure of anti-conf ...
Cavia porcellus
J. Biochem.
140
457-466
2006
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
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-
-
-
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-
-
-
-
-
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-
-
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1
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1
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656219
Hori
Structural basis of leukotrien ...
Cavia porcellus
J. Biol. Chem.
279
22615-22623
2004
-
-
-
1
-
-
-
-
-
-
-
1
-
1
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1
-
-
-
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1
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1
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390829
Yamamoto
Immunohistochemical localizati ...
Cavia porcellus
Eur. J. Biochem.
268
6105-6113
2001
-
-
1
-
-
-
-
2
-
-
-
-
-
8
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
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1
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2
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1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390830
Dick
Antioxidative function and sub ...
Rattus norvegicus
J. Biol. Chem.
276
40803-40810
2001
-
-
1
-
-
-
-
20
-
-
-
-
-
3
-
-
1
-
-
-
-
-
20
-
-
-
-
20
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
20
-
-
-
-
-
-
-
1
-
-
-
-
20
-
-
-
-
20
-
-
-
-
-
-
-
-
-
-
390828
Clish
Oxidoreductases in lipoxin A4 ...
Sus scrofa
J. Biol. Chem.
275
25372-25380
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
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-
-
-
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-
1
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1
1
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1
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1
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-
-
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-
-
-
-
-
-
-
-
390827
Ensor
-
Purification, cDNA cloning and ...
Bos taurus, Sus scrofa
Biochem. J.
330
103-108
1998
-
-
1
-
-
-
-
10
-
-
-
-
-
2
-
-
1
-
-
2
2
-
7
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
10
-
-
-
-
-
-
-
1
-
2
2
-
7
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
390825
Nikaido
15-Ketoprostaglandin delta 13- ...
Bos taurus
Curr. Eye Res.
12
17-22
1993
-
-
-
-
-
-
4
-
1
-
2
-
-
1
-
-
1
-
-
4
6
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
2
-
-
-
-
1
-
4
6
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390826
Kitamura
Purification of a 15-ketoprost ...
Rattus norvegicus
Biochem. Mol. Biol. Int.
30
839-847
1993
-
-
-
-
-
-
4
1
-
-
2
-
-
1
-
-
1
-
-
10
1
-
22
1
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
2
-
-
-
-
1
-
10
1
-
22
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
390820
Hansen
Purification and assay of 15-k ...
Bos taurus, Gallus gallus, Homo sapiens
Methods Enzymol.
86
156-167
1982
-
-
-
-
-
-
3
7
-
-
2
6
-
3
-
-
1
-
-
3
1
2
15
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
3
-
7
-
-
2
6
-
-
-
1
-
3
1
2
15
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390821
Jarabak
Isolation and properties of a ...
Homo sapiens
Methods Enzymol.
86
163-167
1982
-
-
-
-
-
-
3
7
-
-
-
-
-
1
-
-
1
-
-
1
1
2
7
-
1
-
-
-
-
-
-
3
2
-
-
-
-
-
3
-
-
-
-
3
2
7
-
-
-
-
-
-
-
1
-
1
1
2
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390823
Hansen
Purification and characterizat ...
Bos taurus
Biochim. Biophys. Acta
574
136-145
1979
-
-
-
-
-
-
2
2
-
-
1
-
-
1
-
-
1
-
-
1
1
1
2
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
1
1
1
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
390824
Westbrook
15-Ketoprostaglandin delta13 r ...
Homo sapiens
Arch. Biochem. Biophys.
185
429-442
1978
-
-
-
-
-
-
5
7
-
-
2
-
-
1
-
-
1
-
-
1
1
1
8
1
1
-
-
-
-
-
-
2
3
-
-
-
-
-
2
-
-
-
-
5
3
7
-
-
2
-
-
-
-
1
-
1
1
1
8
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390822
Westbrook
Purification and partial chara ...
Homo sapiens
Biochem. Biophys. Res. Commun.
66
541-546
1975
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
2
1
-
3
-
1
-
-
-
1
1
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2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
2
1
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-