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Literature summary for 1.3.1.48 extracted from

  • Nikaido, H.; Moriishi, M.; Mishima, H.K.; Kitamura, S.; Tatsumi, K.
    15-Ketoprostaglandin delta 13-reductase activity in bovine ocular tissues (1993), Curr. Eye Res., 12, 17-22.
    View publication on PubMed
Search for more literature for 1.3.1.48

Inhibitors

Inhibitors Comment Organism Structure
dicoumarol 0.1 mM, 86% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus
Disulfiram 0.1 mM, 61% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus
indomethacin 0.1 mM, 97% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus
quercitrin 0.1 mM, 76% inhibition, cofactor NADPH, substrate 15-ketoprostaglandin E2 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Bos taurus
57000
-
 1 * 57000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
ciliary body
-
 Bos taurus
-
cornea
-
 Bos taurus
-
iris
-
 Bos taurus
-
retina retinal pigment epithelium choroid Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00026
-
 enzyme activity in cornea, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
0.000389
-
 enzyme activity in retina, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
0.00085
-
 enzyme activity in retinal pigment epithelium, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
0.000945
-
 enzyme purified from iris-ciliary cytosol Bos taurus
0.0012
-
 enzyme activity in ciliary body, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus
0.00145
-
 enzyme activity in iris, substrate 15-ketoprostaglandin, cofactor NADPH Bos taurus

Storage Stability

Storage Stability Organism
-80°C, NADH-linked activity is lost overnight Bos taurus
0°C, NADPH-linked activity is lost after a few hours Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H
-
 Bos taurus 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD(P)H enzyme from iris-ciliary body prefers NADPH Bos taurus