Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bos taurus | |
p-chloromercuribenzoate | 0.1 mM, complete inhibition | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | 0.009 | NADPH | - |
Bos taurus | |
0.088 | 0.094 | NADH | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.067 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-4°C, 0.1 M sodium phosphate, pH 7.4, 1 mM EDTA, 1 mM mercaptoethanol, stable for more than 1 month | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | no substrate: prostaglandin E1 | Bos taurus | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
15-ketoprostaglandin E + NAD(P)H | - |
Bos taurus | ? | - |
? | |
15-ketoprostaglandin E2 + NADH + H+ | - |
Bos taurus | 13,14-dihydro-15-ketoprostaglandin E2 + NAD+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | 1 | 15-ketoprostaglandin E | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | bovine lung: enzyme can use both NADPH and NADH | Bos taurus |