BRENDA - Enzyme Database show
show all sequences of 1.3.1.45

Isolation of NADPH:isoflavone oxidoreductase, a new enzyme of pterocarpan phtoalexin biosynthesi in cell suspension cultures of Cicer arietinum

Tiemann, K.; Hinderer, W.; Barz, W.; FEBS Lett. 213, 324-328 (1987)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
2'-hydroxyformononetin
-
Cicer arietinum
0.035
-
NADPH
-
Cicer arietinum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Cicer arietinum
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2'-hydroxyformononetin + NADPH
Cicer arietinum
involved in medicarpin and maackiain biosynthesis
vestitone + NADP+
-
Cicer arietinum
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cicer arietinum
-
chickpea
-
Oxidation Stability
Oxidation Stability
Organism
complete inactivation without dithioerythritol in 6 h at 4°C
Cicer arietinum
Purification (Commentary)
Commentary
Organism
partial
Cicer arietinum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Cicer arietinum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.012
-
-
Cicer arietinum
Storage Stability
Storage Stability
Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 1 mM dithioerythritol, 5% glycerol
Cicer arietinum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',7-dihydroxy-3',4'-methylenedioxyisoflavone + NADPH
-
390812
Cicer arietinum
2',7-hydroxy-dihydro-3',4'-methylenedioxyisoflavone + NADP+
-
390812
Cicer arietinum
?
2'-hydroxyformononetin + NADPH
no reaction with isoflavones lacking 2'-hydroxy-group
390812
Cicer arietinum
(3R)-vestitone + NADP+
-
390812
Cicer arietinum
?
2'-hydroxyformononetin + NADPH
involved in medicarpin and maackiain biosynthesis
390812
Cicer arietinum
vestitone + NADP+
-
390812
Cicer arietinum
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
40
-
Cicer arietinum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Cicer arietinum
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
25-40% of activity with NADPH
Cicer arietinum
NADPH
-
Cicer arietinum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
25-40% of activity with NADPH
Cicer arietinum
NADPH
-
Cicer arietinum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.013
-
2'-hydroxyformononetin
-
Cicer arietinum
0.035
-
NADPH
-
Cicer arietinum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Cicer arietinum
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2'-hydroxyformononetin + NADPH
Cicer arietinum
involved in medicarpin and maackiain biosynthesis
vestitone + NADP+
-
Cicer arietinum
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
complete inactivation without dithioerythritol in 6 h at 4°C
Cicer arietinum
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Cicer arietinum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Cicer arietinum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.012
-
-
Cicer arietinum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 20 mM potassium phosphate buffer, pH 7.5, 1 mM dithioerythritol, 5% glycerol
Cicer arietinum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2',7-dihydroxy-3',4'-methylenedioxyisoflavone + NADPH
-
390812
Cicer arietinum
2',7-hydroxy-dihydro-3',4'-methylenedioxyisoflavone + NADP+
-
390812
Cicer arietinum
?
2'-hydroxyformononetin + NADPH
no reaction with isoflavones lacking 2'-hydroxy-group
390812
Cicer arietinum
(3R)-vestitone + NADP+
-
390812
Cicer arietinum
?
2'-hydroxyformononetin + NADPH
involved in medicarpin and maackiain biosynthesis
390812
Cicer arietinum
vestitone + NADP+
-
390812
Cicer arietinum
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
40
-
Cicer arietinum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Cicer arietinum
Other publictions for EC 1.3.1.45
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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726152
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Ginkgo biloba
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2
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1
2
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746131
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1
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1
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4
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2
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2
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1
3
3
1
-
-
700650
Kim
Overexpression of rice isoflav ...
Oryza sativa
Physiol. Plant.
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1-9
2009
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1
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-
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-
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2
-
3
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1
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4
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2
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1
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1
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2
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1
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6
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2
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2
1
1
4
-
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700726
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Coffea arabica
Plant Cell Rep.
28
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2009
-
-
1
-
-
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1
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3
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2
1
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1
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1
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1
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-
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1
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1
-
-
713596
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Molecular cloning and characte ...
Fagopyrum cymosum
Yao Xue Xue Bao
44
809-819
2009
-
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1
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1
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1
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3
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1
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1
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1
1
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1
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1
1
-
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-
675379
Wang
Crystal structure of isoflavon ...
Medicago sativa
J. Mol. Biol.
358
1341-1352
2006
-
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1
1
8
-
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7
-
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3
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1
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1
1
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7
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1
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1
1
1
8
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7
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1
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1
1
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7
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654910
Saunders
-
The characterization of defens ...
Medicago sativa
BioControl
49
715-728
2004
1
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390816
Cooper
-
Biotransformation of an exogen ...
Medicago sativa
Plant Cell Rep.
20
876-884
2002
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1
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1
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656914
Lopez-Meyer
-
Immunolocalization of vestiton ...
Medicago sativa
Physiol. Mol. Plant Pathol.
61
15-30
2002
1
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1
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1
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6
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390815
Paiva
Molecular cloning of isoflavon ...
Pisum sativum
Arch. Biochem. Biophys.
312
501-510
1994
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1
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5
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390813
Schlieper
-
Stereospecificity of hydrogen ...
Cicer arietinum
Phytochemistry
29
1519-1524
1990
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2
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390814
Preisig
Biosynthesis of the phytoalexi ...
Pisum sativum
Plant Physiol.
94
1444-1448
1990
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390812
Tiemann
-
Isolation of NADPH:isoflavone ...
Cicer arietinum
FEBS Lett.
213
324-328
1987
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