Cloned (Comment) | Organism |
---|---|
phage library screening, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression as His-tagged enzyme in Escherichia coli | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
crotonyl-CoA | pH 6.2, 30°C | Euglena gracilis | |
0.068 | - |
crotonyl-CoA | recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor | Euglena gracilis | |
0.091 | - |
trans-2-hexenoyl-CoA | pH 6.2, 30°C | Euglena gracilis | |
0.091 | - |
trans-2-hexenoyl-CoA | recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor | Euglena gracilis | |
0.109 | - |
Biochanin A | recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate | Euglena gracilis | |
0.109 | - |
Biochanin A | cosubstrate crotonyl-CoA, pH 6.2, 30°C | Euglena gracilis | |
0.119 | - |
NADPH | recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate | Euglena gracilis | |
0.119 | - |
NADPH | cosubstrate crotonyl-CoA, pH 6.2, 30°C | Euglena gracilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Euglena gracilis | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Euglena gracilis | enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation | ? | - |
? | |
trans-didehydroacyl-CoA + NAD(P)H | Euglena gracilis | i.e. enoyl-CoA | acyl-CoA + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | Q5EU90 | - |
- |
Euglena gracilis | Q5EU90 | mitochondrial precursor | - |
Purification (Comment) | Organism |
---|---|
- |
Euglena gracilis |
native enzyme 1687fold to homogeneity in several steps, recombinant His-tagged enzyme from Escherichia coli by affinity chromatography | Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme is expressed under aerobic and anaerobic conditions | Euglena gracilis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.078 | - |
mitochondria, anaerobic conditions, substrates crotonyl-CoA and NADH | Euglena gracilis |
0.086 | - |
mitochondria, aerobic conditions, substrates crotonyl-CoA and NADH | Euglena gracilis |
0.37 | - |
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADPH | Euglena gracilis |
0.66 | - |
purified recombinant enzyme, substrates crotonyl-CoA and NADPH | Euglena gracilis |
1.45 | - |
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADH | Euglena gracilis |
1.65 | - |
purified recombinant enzyme, substrates crotonyl-CoA and NADH | Euglena gracilis |
3.88 | - |
pH 6.2, 30°C | Euglena gracilis |
3.88 | - |
purified native enzyme, substrate crotonyl-CoA and NADH | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + NAD(P)H | - |
Euglena gracilis | butyryl-CoA + NAD(P)+ | - |
? | |
crotonyl-CoA + NADH + H+ | - |
Euglena gracilis | butyryl-CoA + NAD+ | - |
r | |
additional information | enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation | Euglena gracilis | ? | - |
? | |
trans-2-hexenoyl-CoA + NAD(P)H | - |
Euglena gracilis | hexanoyl-CoA + NAD(P)+ | - |
? | |
trans-didehydroacyl-CoA + NAD(P)H | i.e. enoyl-CoA | Euglena gracilis | acyl-CoA + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Euglena gracilis |
monomer | 1 x 44000, SDS-PAGE | Euglena gracilis |
Synonyms | Comment | Organism |
---|---|---|
TER | - |
Euglena gracilis |
trans-2-enoyl-CoA reductase | - |
Euglena gracilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
assay at | Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | preferred, 2-3fold higher activity compared to NADPH | Euglena gracilis | |
NADH | about two-to threefold higher specific activites with NADH over NADPH | Euglena gracilis | |
NADPH | 2-3fold lower activity compared to the preferred NADH | Euglena gracilis | |
NADPH | about two-to threefold higher specific activites with NADH over NADPH | Euglena gracilis |