Any feedback?
Literature summary for 1.3.1.44 extracted from
- Involvement of one of two enoyl-CoA hydratases and enoyl-CoA reductase in the acetyl-CoA-dependent elongation of medium chain fatty acids by Mycobacterium smegmatis (1980), J. Biochem., 88, 1051-1058.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | substrates with chain-length higher than C16, concentrations above 0.02 mM | Mycolicibacterium smegmatis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
NADH | - |
Mycolicibacterium smegmatis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + NAD+ | Mycolicibacterium smegmatis | fatty acid elongation | trans-2,3-didehydroacyl-CoA + NADH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.351 | - |
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + NAD+ | fatty acid elongation | Mycolicibacterium smegmatis | trans-2,3-didehydroacyl-CoA + NADH | - |
? | |
| additional information | inert to substrates with chain length below C8 | Mycolicibacterium smegmatis | ? | - |
? | |
| trans-2-decenoyl-CoA + NADH | - |
Mycolicibacterium smegmatis | decanoyl-CoA + NAD+ | - |
? | |
| trans-2-dodecenoyl-CoA + NADH | - |
Mycolicibacterium smegmatis | dodecanoyl-CoA + NAD+ | - |
? | |
| trans-2-hexadecenoyl-CoA + NADH | - |
Mycolicibacterium smegmatis | hexadecanoyl-CoA + NAD+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Mycolicibacterium smegmatis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | sole electron donor | Mycolicibacterium smegmatis | |
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Release 2023.1 (January 2023)
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