Literature summary for 1.3.1.42 extracted from

  • Li, W.; Zhou, F.; Liu, B.; Feng, D.; He, Y.; Qi, K.; Wang, H.; Wang, J.
    Comparative characterization, expression pattern and function analysis of the 12-oxo-phytodienoic acid reductase gene family in rice (2011), Plant Cell Rep., 30, 981-995.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.115
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
0.134
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
0.148
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
0.0934
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
0.05283
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
callus abundant expression Oryza sativa
-
leaf abundant expression Oryza sativa
-
additional information weak expression in root, stem, callus, panicle Oryza sativa
-
panicle abundant expression Oryza sativa
-
root abundant expression Oryza sativa
-
seed abundant expression Oryza sativa
-
stem abundant expression Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2-hexenal + NADPH + H+
-
Oryza sativa hexanal + NADP+
-
?
2-cyclohexen-1-ol + NADPH
-
Oryza sativa ? + NADP+
-
?
maleic acid + NADPH
-
Oryza sativa ?
-
?
additional information no activity towards fumaric acid, cyclohexanone and hexanal Oryza sativa ?
-
?
additional information no activity towards fumaric acid, cyclohexanone, isomeride maleic acid and hexanal Oryza sativa ?
-
?
racemic 12-oxo-phytodienoic acid + NADPH
-
Oryza sativa ?
-
?

Synonyms

Synonyms Comment Organism
12-oxo-phytodienoic acid reductase
-
Oryza sativa
OsOPR01-1
-
Oryza sativa
OsOPR02-1
-
Oryza sativa
OsOPR04-1
-
Oryza sativa
OsOPR06-1
-
Oryza sativa
OsOPR08-1
-
Oryza sativa
OsOPR10
-
Oryza sativa
OsOPR11
-
Oryza sativa
OsOPR6
-
Oryza sativa
OsOPR7
-
Oryza sativa
OsOPR8
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.94
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
8.65
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
9.58
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
9.65
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa
10.22
-
2-Cyclohexen-1-ol pH 7.5, 25°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Oryza sativa