Literature summary for 1.3.1.42 extracted from

  • Malone, T.E.; Madson, S.E.; Wrobel, R.L.; Jeon, W.B.; Rosenberg, N.S.; Johnson, K.A.; Bingman, C.A.; Smith, D.W.; Phillips, G.N., Jr.; Markley, J.L.; Fox, B.G.
    X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3 (2004), Proteins, 58, 243-245.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH Arabidopsis thaliana product is an intermediate in the jasmonate biosynthesis (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
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?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
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isozyme OPR3
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Purification (Commentary)

Purification (Comment) Organism
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
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Arabidopsis thaliana (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH product is an intermediate in the jasmonate biosynthesis Arabidopsis thaliana (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?

Synonyms

Synonyms Comment Organism
OPR
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Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADPH
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Arabidopsis thaliana