Literature summary for 1.3.1.42 extracted from

  • Strassner, J.; Schaller, F.; Frick, U.B.; Howe, G.A.; Weiler, E.W.; Amrhein, N.; Macheroux, P.; Schaller, A.
    Characterization and cDNA-microarray expression analysis of 12-oxophytodienoate reductases reveals differential roles for octadecanoid biosynthesis in the local versus the systemic wound response (2002), Plant J., 32, 585-601.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study Solanum lycopersicum
expression of His-tagged isozyme OPR2 in Escherichia coli Solanum lycopersicum
expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study Solanum lycopersicum
expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli Arabidopsis thaliana

Engineering

Protein Variants Comment Organism
additional information construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome isozyme OPR3 Arabidopsis thaliana
-
-
peroxisome isozyme OPR3 Solanum lycopersicum
-
-
cytosol isozyme OPR1 Solanum lycopersicum
-
-
cytosol isozyme OPR2 Solanum lycopersicum
-
-
cytosol isozymes OPR1 and OPR2 Arabidopsis thaliana
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Arabidopsis thaliana
Mg2+
-
Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum lycopersicum phylogenetic analysis ?
-
?
additional information Solanum lycopersicum phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response ?
-
?
additional information Arabidopsis thaliana phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis ?
-
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH Arabidopsis thaliana
-
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH Solanum lycopersicum
-
8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH Solanum lycopersicum
-
(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH Solanum lycopersicum product is the direct precursor in the jasmonate biosynthesis (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
ecotype Col-0, 3 isozymes OPR1-3
-
Solanum lycopersicum Q9FEW9 isozyme OPR3; 3 isozymes OPR1-3
-
Solanum lycopersicum Q9FEX0 isozyme OPR2, 12-oxophytodienoate-like protein; 3 isozymes OPR1-3, 12-oxophytodienoate-like protein
-
Solanum lycopersicum Q9XG54 isozyme OPR1; 3 isozymes OPR1-3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum
recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum
recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography Solanum lycopersicum
recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf isozyme OPR3, up-regulation in wounded leaves, low activity in unwounded, systemic leaves Arabidopsis thaliana
-
shoot
-
Arabidopsis thaliana
-
mesophyll
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phylogenetic analysis Solanum lycopersicum ?
-
?
additional information phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response Solanum lycopersicum ?
-
?
additional information phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis Arabidopsis thaliana ?
-
?
additional information no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers Solanum lycopersicum ?
-
?
additional information stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds Arabidopsis thaliana ?
-
?
additional information stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds Solanum lycopersicum ?
-
?
additional information stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds Solanum lycopersicum ?
-
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
-
Arabidopsis thaliana 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH
-
Solanum lycopersicum 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(15Z)-12-oxophyto-10,15-dienoate + NADPH racemic mixture as substrate is accepted by isozyme OPR1 Arabidopsis thaliana 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH
-
Solanum lycopersicum (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH isozyme OPR1, no activity with the (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate enantiomer Solanum lycopersicum (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH isozymes OPR3 Solanum lycopersicum (9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH product is the direct precursor in the jasmonate biosynthesis Solanum lycopersicum (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH isozyme OPR3 Solanum lycopersicum (9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+
-
?

Synonyms

Synonyms Comment Organism
OPR
-
Arabidopsis thaliana
OPR
-
Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana
25
-
assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Arabidopsis thaliana
7.5
-
assay at Solanum lycopersicum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Arabidopsis thaliana
NADPH
-
Solanum lycopersicum
flavin dependent on Arabidopsis thaliana
flavin dependent on Solanum lycopersicum