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Literature summary for 1.3.1.42 extracted from
- 12-Oxophytodienoate reductase 3 (OPR3) is the isoenzyme involved in jasmonate biosynthesis (2000), Planta, 210, 979-984.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
(9S,13S)-12-oxophytodienoic acid | - |
Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9S,13S)-12-oxo-phytodienoic acid + NADPH | Arabidopsis thaliana | OPR3 is the isoenzyme relevant for jasmonate biosynthesis | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
| (9S,13S)-12-oxo-phytodienoic acid + NADPH | Capnoides sempervirens | reductase specific for the natural cis-OPDA enatiomer encoded by OPR2 | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
(L.) Heynta wild-type ecotype Columbia(Col-0) | - |
| Capnoides sempervirens | - |
- |
- |
| Saccharomyces cerevisiae | - |
yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
- |
Capnoides sempervirens |
| OPR1, OPR2 and OPR3 | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9S,13S)-12-oxo-phytodienoic acid + NADPH | OPR3 is the isoenzyme relevant for jasmonate biosynthesis | Arabidopsis thaliana | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
| (9S,13S)-12-oxo-phytodienoic acid + NADPH | reductase specific for the natural cis-OPDA enatiomer encoded by OPR2 | Capnoides sempervirens | 3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPR1 | - |
Arabidopsis thaliana |
| OPR1 | - |
Capnoides sempervirens |
| OPR2 | - |
Arabidopsis thaliana |
| OPR3 | - |
Arabidopsis thaliana |
| OYE | - |
Saccharomyces cerevisiae |
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