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Literature summary for 1.3.1.38 extracted from

  • Bell, A.F.; Stratton, C.F.; Zhang, X.; Novichenok, P.; Jaye, A.A.; Nair, P.A.; Parikh, S.; Rawat, R.; Tonge, P.J.
    Evidence from Raman spectroscopy that InhA, the mycobacterial enoyl reductase, modulates the conformation of the NADH cofactor to promote catalysis (2007), J. Am. Chem. Soc., 129, 6425-6431.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
F149A the mutant is not able to optimally position the cofactor for hydride transfer, which correlates with the 30fold decrease in kcat Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00433
-
2-dodecenoyl-CoA mutant enzyme F149A, at 25┬░C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
0.0054
-
2-dodecenoyl-CoA wild type enzyme, at 25┬░C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
0.0152
-
NADH mutant enzyme F149A, at 25┬░C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
0.0352
-
NADH wild type enzyme, at 25┬░C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dodecenoyl-acyl carrier protein + NADH
-
Mycobacterium tuberculosis dodecanoyl-acyl carrier protein + NAD+
-
?
2-trans,4-trans-decadienoyl-CoA + NADPH + H+
-
Mycobacterium tuberculosis (4E)-dec-4-enoyl-CoA + NADP+
-
?

Synonyms

Synonyms Comment Organism
enoyl reductase
-
Mycobacterium tuberculosis
InhA
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
(4E)-dec-4-enoyl-CoA mutant enzyme F149A, at 25┬░C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis
3.17
-
(4E)-dec-4-enoyl-CoA wild type enzyme, at 25┬░C in 30 mM PIPES and 150 mM NaCl (pH 6.8) Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Mycobacterium tuberculosis