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Literature summary for 1.3.1.38 extracted from

  • Mizugaki, M.; Nishimaki, T.; Shiraishi, T.; Yamanaka, H.
    Studies on the metabolism of unsaturated fatty acids. VII. Separation and general properties of reduced nicotinamide adenine dinucleotide phosphate-dependent trans-2-enoyl-coenzyme A reductase from Escherichia coli K-12 (1982), Chem. Pharm. Bull., 30, 2503-2511.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Escherichia coli
iodoacetic acid
-
Escherichia coli
additional information Zn2+, Fe3+, Mn2+, Mg2+, Ca2+, NH4+, Na+ Escherichia coli
N-ethylmaleimide
-
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-2-hexenoyl-CoA + NADPH + H+ Escherichia coli
-
hexanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH + H+ Escherichia coli
-
octanoyl-CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH + H+ inducible by growth on oleic acid Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.49
-
partially purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20┬░C, pH 7.0 Escherichia coli
0┬░C, pH 7.0, 1 mg per ml, 70% of activity remaining after 44 h Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no reduction of trans-2-tetradecenoyl-CoA Escherichia coli ?
-
?
trans-2-hexenoyl-CoA + NADPH
-
Escherichia coli hexanoyl-CoA + NADP+
-
?
trans-2-hexenoyl-CoA + NADPH + H+
-
Escherichia coli hexanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH
-
Escherichia coli octanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH + H+
-
Escherichia coli octanoyl-CoA + NADP+
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
15 min, pH 6.0-8.0 Escherichia coli
56
-
10 min, pH 6.0, 50% inactivation but reactivation at pH 8.0 Escherichia coli
60
-
10 min, inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Escherichia coli