Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.38 extracted from

  • Nishimaki, T.; Yamanaka, H.; Mizugaki, M.
    Studies on the metabolism of unsaturated fatty acids. XIV. Purification and properties of NADPH-dependent trans-2-enoyl-CoA reductase of Escherichia coli K-12 (1984), J. Biochem., 95, 1315-1321.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no flavoprotein Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) i.e. DTNB, inhibition can be prevented by preincubation with NADPH, thus a SH-group is involved in cofactor binding Escherichia coli
bovine serum albumin
-
Escherichia coli
decanoyl-CoA
-
Escherichia coli
lauroyl-CoA
-
Escherichia coli
additional information not inhibitory: methylglyoxal Escherichia coli
myristoyl-CoA
-
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
NADP+
-
Escherichia coli
octanoyl-CoA
-
Escherichia coli
p-hydroxymercuribenzoate
-
Escherichia coli
palmitoleoyl-CoA
-
Escherichia coli
palmitoyl-CoA
-
Escherichia coli
Saturated CoA analogs
-
Escherichia coli
Triton X-100
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
trans-2-dodecenoyl-CoA
-
Escherichia coli
0.0049
-
NADPH
-
Escherichia coli
0.0071
-
trans-2-decenoyl-CoA
-
Escherichia coli
0.011
-
trans-2-octenoyl-CoA
-
Escherichia coli
0.048
-
trans-2-hexenoyl-CoA
-
Escherichia coli
0.072
-
trans-2-crotonyl-CoA
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Escherichia coli
80000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-2-hexenoyl-CoA + NADPH + H+ Escherichia coli
-
hexanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH + H+ Escherichia coli
-
octanoyl-CoA + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K12
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
20
-
purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20Ā°C, Tris-HCl buffer, pH 8.0, 10% v/v glycerol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NADPH
-
Escherichia coli butanoyl-CoA + NADP+
-
?
additional information activity decreases with increasing chain length of substrates Escherichia coli ?
-
?
trans-2-decenoyl-CoA + NADPH
-
Escherichia coli decanoyl-CoA + NADP+
-
?
trans-2-dodecenoyl-CoA
-
Escherichia coli ?
-
r
trans-2-hexenoyl-CoA + NADPH
-
Escherichia coli hexanoyl-CoA + NADP+
-
?
trans-2-hexenoyl-CoA + NADPH + H+
-
Escherichia coli hexanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH
-
Escherichia coli octanoyl-CoA + NADP+
-
?
trans-2-octenoyl-CoA + NADPH + H+
-
Escherichia coli octanoyl-CoA + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH specific for Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00054
-
palmitoleoyl-CoA
-
Escherichia coli
0.0025
-
palmitoyl-CoA
-
Escherichia coli
0.0092
-
myristoyl-CoA
-
Escherichia coli
0.045
-
lauroyl-CoA
-
Escherichia coli
0.063
-
decanoyl-CoA
-
Escherichia coli
0.198
-
octanoyl-CoA
-
Escherichia coli