BRENDA - Enzyme Database
show all sequences of 1.3.1.34

Expression, purification, and characterization of His-tagged human mitochondrial 2,4-dienoyl-CoA reductase

Chu, X.; Yu, W.; Chen, G.; Li, D.; Protein Expr. Purif. 31, 292-297 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
functional overexpression of truncated His-tagged enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
2,4-trans-decadienoyl-CoA
recombinant His-tagged truncated enzyme, pH 6.0, 22°C
Homo sapiens
0.0265
-
2,4-trans-hexadienoyl-CoA
recombinant His-tagged truncated enzyme, pH 6.0, 22°C
Homo sapiens
0.0605
-
NADPH
recombinant His-tagged truncated enzyme, pH 6.0, 22°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Homo sapiens
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Homo sapiens
key enzyme in the beta-oxidation of unsaturated fatty acids
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity
Homo sapiens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.21
-
purified recombinant truncated His-tagged enzyme, substrate 2,4-trans-hexadienoyl-CoA
Homo sapiens
Storage Stability
Storage Stability
Organism
-80°C, purified recombinant truncated enzyme, completely stable for at least 6 months
Homo sapiens
22°C, room temperature, purified recombinant truncated enzyme, 70% remaining activity after 1 week
Homo sapiens
4°C, purified recombinant truncated enzyme, completely stable for 1 week
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,4-trans-decadienoyl-CoA + NADPH
-
657265
Homo sapiens
? + NADP+
-
-
-
?
2,4-trans-hexadienoyl-CoA + NADPH
-
657265
Homo sapiens
trans-3-hexenoyl-CoA + NADP+
-
-
-
?
additional information
key enzyme in the beta-oxidation of unsaturated fatty acids
657265
Homo sapiens
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE
Homo sapiens
Synonyms
Synonyms
Commentary
Organism
2,4-dienoyl-CoA reductase
-
Homo sapiens
DECR
-
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Homo sapiens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
22
-
purified recombinant truncated enzyme is highly stable at room temperature
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
functional overexpression of truncated His-tagged enzyme in Escherichia coli strain BL21(DE3)
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0062
-
2,4-trans-decadienoyl-CoA
recombinant His-tagged truncated enzyme, pH 6.0, 22°C
Homo sapiens
0.0265
-
2,4-trans-hexadienoyl-CoA
recombinant His-tagged truncated enzyme, pH 6.0, 22°C
Homo sapiens
0.0605
-
NADPH
recombinant His-tagged truncated enzyme, pH 6.0, 22°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Homo sapiens
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Homo sapiens
key enzyme in the beta-oxidation of unsaturated fatty acids
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.21
-
purified recombinant truncated His-tagged enzyme, substrate 2,4-trans-hexadienoyl-CoA
Homo sapiens
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, purified recombinant truncated enzyme, completely stable for at least 6 months
Homo sapiens
22°C, room temperature, purified recombinant truncated enzyme, 70% remaining activity after 1 week
Homo sapiens
4°C, purified recombinant truncated enzyme, completely stable for 1 week
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,4-trans-decadienoyl-CoA + NADPH
-
657265
Homo sapiens
? + NADP+
-
-
-
?
2,4-trans-hexadienoyl-CoA + NADPH
-
657265
Homo sapiens
trans-3-hexenoyl-CoA + NADP+
-
-
-
?
additional information
key enzyme in the beta-oxidation of unsaturated fatty acids
657265
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34000, recombinant truncated His-tagged enzyme, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Homo sapiens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
22
-
purified recombinant truncated enzyme is highly stable at room temperature
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Homo sapiens
Other publictions for EC 1.3.1.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725482
Hua
Studies of human 2,4-dienoyl C ...
Homo sapiens
J. Biol. Chem.
287
28956-28965
2012
-
-
1
1
6
-
-
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
6
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685196
Tu
Two distinct proton donors at ...
Escherichia coli
Biochemistry
47
1167-1175
2008
-
-
1
-
8
-
-
6
-
1
-
-
-
6
-
-
1
-
-
-
7
-
2
-
1
-
-
-
6
-
-
-
2
-
-
-
-
-
1
2
-
8
-
-
-
-
6
-
1
-
-
-
-
-
1
-
-
7
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
652365
Ren
Metabolic functions of the two ...
Rattus norvegicus
J. Biol. Chem.
278
111-116
2003
-
-
-
-
-
-
1
1
1
-
-
2
-
2
-
-
1
-
-
1
-
-
4
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
-
2
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
656161
Hubbard
The crystal structure and reac ...
Escherichia coli
J. Biol. Chem.
278
37553-37560
2003
-
-
1
1
-
-
-
-
-
1
-
-
-
9
-
-
1
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657265
Chu
Expression, purification, and ...
Homo sapiens
Protein Expr. Purif.
31
292-297
2003
-
-
1
-
-
-
-
3
3
-
1
1
-
8
-
-
1
-
-
-
1
3
3
1
2
1
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
3
-
1
1
-
-
-
1
-
-
1
3
3
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
390725
De Nys
Characterisation of human pero ...
Homo sapiens
Biochim. Biophys. Acta
1533
66-72
2001
-
-
1
-
-
-
1
3
4
-
1
-
-
7
-
-
-
-
-
1
-
1
4
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
3
4
-
1
-
-
-
-
-
-
1
-
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
390724
Liang
2,4-Dienoyl-CoA reductase from ...
Escherichia coli
Arch. Biochem. Biophys.
380
373-379
2000
-
-
1
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390720
Gruvitz
The Saccharomyces cerevisiae p ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
22140-22147
1997
-
-
1
-
-
-
-
-
2
-
2
-
-
11
-
-
1
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390721
He
Cloning and expression of the ...
Escherichia coli
Eur. J. Biochem.
248
516-520
1997
-
-
1
-
-
-
-
2
-
-
1
-
-
6
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
390708
Mizugaki
Studies on the metabolism of u ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biochem.
91
1453-1456
1982
-
-
-
-
-
-
2
-
-
-
-
-
-
182
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
1
-
3
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
390710
Dommes
Purification by affinity chrom ...
Bos taurus, Escherichia coli
Eur. J. Biochem.
125
335-341
1982
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
2
-
-
2
2
-
2
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
2
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-