BRENDA - Enzyme Database
show all sequences of 1.3.1.34

Cloning and expression of the fadH gene and characterization of the gene product 2,4-dienoyl coenzyme A reductase from Escherichia coli

He, X.Y.; Yang, S.Y.; Schulz, H.; Eur. J. Biochem. 248, 516-520 (1997)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
2-trans,4-trans-decadienoyl-CoA
-
Escherichia coli
0.05
-
NADPH
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72550
-
purified recombinant enzyme, encoded by the gene fadH, SDS-PAGE
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-trans,4-trans-decadienoyl-CoA + NADPH
-
390721
Escherichia coli
3-decenoyl-CoA + NADP+
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
2,4-dienoyl-CoA reductase
-
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
16
-
2-trans,4-trans-decadienoyl-CoA
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
2-trans,4-trans-decadienoyl-CoA
-
Escherichia coli
0.05
-
NADPH
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72550
-
purified recombinant enzyme, encoded by the gene fadH, SDS-PAGE
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-trans,4-trans-decadienoyl-CoA + NADPH
-
390721
Escherichia coli
3-decenoyl-CoA + NADP+
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
16
-
2-trans,4-trans-decadienoyl-CoA
-
Escherichia coli
Other publictions for EC 1.3.1.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726092
Satoh
Overexpression of hydroxymethy ...
Rattus norvegicus
Pancreas
42
475-482
2013
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1
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1
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740563
Li
Effects of pectin pentaoligosa ...
Mus musculus
J. Agric. Food Chem.
61
7599-7605
2013
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725482
Hua
Studies of human 2,4-dienoyl C ...
Homo sapiens
J. Biol. Chem.
287
28956-28965
2012
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1
1
6
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2
1
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1
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6
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2
1
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697264
Di Costanzo
Aldo-keto reductases in which ...
Digitalis lanata
Chem. Biol. Interact.
178
127-133
2009
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1
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2
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700872
Miinalainen
Mitochondrial 2,4-dienoyl-CoA ...
Mus musculus
PLOS Genet.
5
e1000543
2009
-
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1
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3
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3
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4
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3
4
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3
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3
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1
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1
1
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3
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3
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3
4
-
3
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685196
Tu
Two distinct proton donors at ...
Escherichia coli
Biochemistry
47
1167-1175
2008
-
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1
-
8
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6
-
1
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3
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1
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7
-
2
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1
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6
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2
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1
2
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8
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6
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1
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1
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7
-
2
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6
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654376
Yu
Studies of human mitochondrial ...
Homo sapiens
Arch. Biochem. Biophys.
434
195-200
2005
-
-
1
-
5
-
1
12
2
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1
-
4
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1
1
1
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4
-
1
1
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3
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1
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1
1
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5
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1
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12
2
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1
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1
1
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4
-
1
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3
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656286
Alphey
Structure and reactivity of hu ...
Homo sapiens
J. Biol. Chem.
280
3068-3077
2005
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1
1
7
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2
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2
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3
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1
1
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3
1
3
1
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1
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2
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1
2
1
7
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2
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2
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1
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3
1
1
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1
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661257
Robert
Analysis of the beta-oxidation ...
Saccharomyces cerevisiae
BIOCHIM. BIOPHYS. ACTA
1734
169-177
2005
-
-
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1
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2
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652365
Ren
Metabolic functions of the two ...
Rattus norvegicus
J. Biol. Chem.
278
111-116
2003
-
-
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-
-
1
1
1
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2
-
2
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1
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1
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4
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1
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1
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1
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1
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1
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4
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1
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656161
Hubbard
The crystal structure and reac ...
Escherichia coli
J. Biol. Chem.
278
37553-37560
2003
-
-
1
1
-
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-
-
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1
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3
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1
1
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657265
Chu
Expression, purification, and ...
Homo sapiens
Protein Expr. Purif.
31
292-297
2003
-
-
1
-
-
-
-
3
2
-
1
1
-
3
-
-
1
-
-
-
1
3
3
1
2
1
-
1
-
1
-
-
1
-
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-
1
1
-
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-
-
-
3
2
-
1
1
-
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1
-
-
1
3
3
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
390725
De Nys
Characterisation of human pero ...
Homo sapiens
Biochim. Biophys. Acta
1533
66-72
2001
-
-
1
-
-
-
1
3
3
-
1
-
-
3
-
-
-
-
-
1
-
1
4
-
1
1
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1
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1
1
-
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1
-
3
3
-
1
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1
-
1
4
-
1
-
-
-
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-
-
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-
-
-
-
-
390724
Liang
2,4-Dienoyl-CoA reductase from ...
Escherichia coli
Arch. Biochem. Biophys.
380
373-379
2000
-
-
1
-
-
-
-
-
-
1
-
-
-
2
-
-
-
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-
-
-
-
4
-
1
-
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-
-
-
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-
3
-
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-
1
3
-
-
-
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1
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-
-
-
-
-
4
-
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390722
Fillgrove
Cloning, expression, and purif ...
Rattus norvegicus
Protein Expr. Purif.
17
57-63
1999
-
-
1
-
-
-
-
2
2
-
1
-
-
3
-
-
1
-
-
1
1
-
2
-
1
-
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-
1
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1
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1
1
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2
2
-
1
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1
-
1
1
-
2
-
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-
1
-
-
-
-
-
-
-
-
-
-
390723
Geisbrecht
The mouse gene PDCR encodes a ...
Mus musculus
J. Biol. Chem.
274
25814-25820
1999
-
-
1
-
-
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-
3
1
-
1
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1
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1
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1
1
3
-
1
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1
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1
1
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3
1
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1
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1
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1
1
3
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390720
Gruvitz
The Saccharomyces cerevisiae p ...
Saccharomyces cerevisiae
J. Biol. Chem.
272
22140-22147
1997
-
-
1
-
-
-
-
-
1
-
2
-
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5
-
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1
-
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1
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1
1
1
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1
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1
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2
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1
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1
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1
1
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390721
He
Cloning and expression of the ...
Escherichia coli
Eur. J. Biochem.
248
516-520
1997
-
-
1
-
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-
2
-
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1
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2
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1
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1
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1
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1
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2
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1
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1
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1
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390719
Mizugaki
Subcellular distribution of ra ...
Rattus norvegicus
Biol. Pharm. Bull.
19
176-181
1996
1
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2
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2
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1
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390718
Koivuranta
-
Isolation and characterization ...
Homo sapiens
Biochem. J.
304
787-792
1994
-
-
1
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1
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1
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1
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1
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1
1
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1
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1
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1
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685405
Hiltunen
Peroxisomal beta-oxidation of ...
Rattus norvegicus
Biochimie
75
175-182
1993
-
-
-
-
-
-
-
-
2
-
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1
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1
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1
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690046
Hiltunen
Peroxisomes and beta-oxidation ...
Rattus norvegicus
Scand. J. Clin. Lab. Invest. Suppl.
204
33-46
1991
1
-
-
-
-
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1
-
2
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1
-
2
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1
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1
2
-
2
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1
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1
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1
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2
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1
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1
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1
2
-
2
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-
390717
Roe
2,4-Dienolyl-coenzyme A reduct ...
Homo sapiens
J. Clin. Invest.
85
1703-1707
1990
-
-
-
-
-
-
1
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1
-
1
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3
4
-
3
-
1
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1
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1
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1
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1
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3
4
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3
-
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-
390707
Clejan
Effect of growth hormone on fa ...
Rattus norvegicus
Arch. Biochem. Biophys.
246
820-828
1986
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1
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2
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1
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1
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390708
Mizugaki
Studies on the metabolism of u ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biochem.
91
1453-1456
1982
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2
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180
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3
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3
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390709
Osmundsen
A role for 2,4-enoyl-CoA reduc ...
Rattus norvegicus
Biochem. J.
208
749-757
1982
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390710
Dommes
Purification by affinity chrom ...
Bos taurus, Escherichia coli
Eur. J. Biochem.
125
335-341
1982
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4
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2
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390711
Dommes
Degradation of unsaturated fat ...
Rattus norvegicus
J. Biol. Chem.
256
8259-8262
1981
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390713
Kunau
Degradation of unsaturated fat ...
Bos taurus
Prog. Lipid Res.
20
327-330
1981
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1
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390714
Hiltunen
Metabolism of pent-4-enoate in ...
Rattus norvegicus, Rattus norvegicus Wistar
Biochem. J.
194
427-432
1981
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168
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390715
Borrebaek
Increased 4-enoyl-CoA reductas ...
Rattus norvegicus
FEBS Lett.
121
23-24
1980
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2
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5
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390716
Borrebaek
In vivo induction of 4-enoyl-C ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
93
1173-1180
1980
1
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390712
Kunau
Degradation of unsaturated fat ...
Bos taurus
Eur. J. Biochem.
91
533-544
1978
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