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Literature summary for 1.3.1.33 extracted from
- Purification and kinetic analysis of pea (Pisum sativum L.) NADPH:protochlorophyllide oxidoreductase expressed as a fusion with maltose-binding protein in Escherichia coli (1997), Biochem. J., 325, 139-145.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed as a fusion with maltose-binding protein in Escherichia coli using expression plasmid pKK233-2 | Pisum sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00019 | - |
protochlorophyllide | - |
Tetradesmus obliquus |  |
| 0.00027 | - |
protochlorophyllide | - |
Pisum sativum | |
| 0.00046 | - |
protochlorophyllide | - |
Hordeum vulgare | |
| 0.0042 | - |
NADPH | - |
Tetradesmus obliquus | |
| 0.00867 | - |
NADPH | - |
Pisum sativum | |
| 0.035 | - |
NADPH | - |
Hordeum vulgare | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | nuclear-encoded cytoplasmically sythesized, posttranslationally processed on import into plastids | Pisum sativum | 5737 | - |
| plastid | - |
Hordeum vulgare | 9536 | - |
| plastid | inner plastid membrane | Pisum sativum | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78800 | - |
recombinant fusion protein, gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | Tetradesmus obliquus | - |
chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Pisum sativum | important regulatory step in chlorophyll biosynthesis | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Hordeum vulgare | chlorophyll-biosynthetic pathway | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | Pisum sativum | chlorophyll-biosynthetic pathway | chlorophyllide + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
barley | - |
| Pisum sativum | - |
pea | - |
| Tetradesmus obliquus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant fusion protein | Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stored in elution buffer retains full activity for many weeks | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protochlorophyllide + NADPH | - |
Tetradesmus obliquus | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Hordeum vulgare | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | - |
Pisum sativum | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | important regulatory step in chlorophyll biosynthesis | Pisum sativum | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | chlorophyll-biosynthetic pathway | Hordeum vulgare | chlorophyllide + NADP+ | - |
? | |
| protochlorophyllide + NADPH | chlorophyll-biosynthetic pathway | Pisum sativum | chlorophyllide + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | recombinant fusion protein, gel filtration | Pisum sativum |
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