Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 1.1-1.4 M ammonium sulfate, 0.1 M sodium chloride, 2% (w/v) benzamidine-HCl and 0.1 M Na HEPES pH 7.5 at 20°C | Rhizobium sp. |
Protein Variants | Comment | Organism |
---|---|---|
C242A | the mutant shows 25% activity compared to the wild type enzyme | Rhizobium sp. |
H243A | the mutant shows 0.4% activity compared to the wild type enzyme | Rhizobium sp. |
Y326A | the mutant shows 6% activity compared to the wild type enzyme | Rhizobium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36406 | - |
strain MTP-10005 | Rhizobium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-maleylacetate + NADH + H+ | Rhizobium sp. | - |
3-oxoadipate + NAD+ | - |
? | |
2-maleylacetate + NADPH + H+ | Rhizobium sp. | - |
3-oxoadipate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium sp. | A1IIX4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.721 | - |
mutant enzyme H243A, at pH 6.5 and 30°C | Rhizobium sp. |
11.7 | - |
mutant enzyme Y326A, at pH 6.5 and 30°C | Rhizobium sp. |
50 | - |
mutant enzyme C242A, at pH 6.5 and 30°C | Rhizobium sp. |
202 | - |
wild type enzyme, at pH 6.5 and 30°C | Rhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-maleylacetate + NADH + H+ | - |
Rhizobium sp. | 3-oxoadipate + NAD+ | - |
? | |
2-maleylacetate + NADPH + H+ | - |
Rhizobium sp. | 3-oxoadipate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36406, calculated from amino acid sequence | Rhizobium sp. |
Synonyms | Comment | Organism |
---|---|---|
GraC | - |
Rhizobium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Rhizobium sp. | |
NADPH | - |
Rhizobium sp. |