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Literature summary for 1.3.1.32 extracted from

  • Chauhan, A.; Pandey, G.; Sharma, N.K.; Paul, D.; Pandey, J.; Jain, R.K.
    p-Nitrophenol degradation via 4-nitrocatechol in Burkholderia sp. SJ98 and cloning of some of the lower pathway genes (2010), Environ. Sci. Technol., 44, 3435-3441.
    View publication on PubMed
Search for more literature for 1.3.1.32

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ralstonia sp.

General Stability

General Stability Organism
highly unstable and prone to precipitation at a concentration of 1 mg/ml or more in 50 mM Tris-Cl (pH 8.0) containing 50 mM NaCl at ambient temperatures. The stability of MaR can be improved by the addition of glycerol (at 30% v/v concentration) or by increasing the NaCl concentration (up to 1 M) Ralstonia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Ralstonia sp.
40000
-
 SDS-PAGE Ralstonia sp.

Organism

Organism UniProt Comment Textmining
Ralstonia sp. Q6PW32
-
-
Ralstonia sp. SJ98 Q6PW32
-
-

Storage Stability

Storage Stability Organism
-20°C, 30% glycerol, 4 months, enzyme retains its activity Ralstonia sp.
-70°C, 30% glycerol, 6 months, enzyme retains its activity Ralstonia sp.
4°C, 30% glycerol, 3 weeks, enzyme retains its activity Ralstonia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-maleylacetate + NADPH
-
 Ralstonia sp. 3-oxoadipate + NADP+
-
 ?
2-maleylacetate + NADPH
-
 Ralstonia sp. SJ98 3-oxoadipate + NADP+
-
 ?

Subunits

Subunits Comment Organism
monomer gel filtration Ralstonia sp.

Synonyms

Synonyms Comment Organism
maleylacetate reductase
-
 Ralstonia sp.
MAR
-
 Ralstonia sp.
PnpD
-
 Ralstonia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Ralstonia sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Ralstonia sp.