Cloned (Comment) | Organism |
---|---|
gene pnpD, expression of N-terminally His-tagged wild-type and SeMet-labeled PnpD in Escherichia coli BL21 (DE3) | Ralstonia sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged wild-type and SeMet-labeled PnpD, the sitting-drop vapour-diffusion method, 0.002 ml of 15 mg/ml protein in 20 mM Tris, pH 8.0, 5 mM NADH, and 20 mM NaCl are mixed with 0.002 ml of precipitant solution containing 0.2 M ammonium phosphate dibasic and 20% w/v PEG 3350, equilibration against 0.2 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.9 A resolution | Ralstonia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39183 | - |
x * 39183, mass spectrometry | Ralstonia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia sp. | Q6PW32 | gene pnpD | - |
Ralstonia sp. SJ98 | Q6PW32 | gene pnpD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and SeMet-labeled PnpD from Escherichia coli BL21 (DE3) by nickel affinity chromatography | Ralstonia sp. |
Subunits | Comment | Organism |
---|---|---|
? | x * 39183, mass spectrometry | Ralstonia sp. |
Synonyms | Comment | Organism |
---|---|---|
PnpD | - |
Ralstonia sp. |