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Literature summary for 1.3.1.32 extracted from

  • Chauhan, A.; Islam, Z.; Jain, R.K.; Karthikeyan, S.
    Expression, purification, crystallization and preliminary X-ray analysis of maleylacetate reductase from Burkholderia sp. strain SJ98 (2009), Acta Crystallogr. Sect. F, 65, 1313-1316.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pnpD, expression of N-terminally His-tagged wild-type and SeMet-labeled PnpD in Escherichia coli BL21 (DE3) Ralstonia sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged wild-type and SeMet-labeled PnpD, the sitting-drop vapour-diffusion method, 0.002 ml of 15 mg/ml protein in 20 mM Tris, pH 8.0, 5 mM NADH, and 20 mM NaCl are mixed with 0.002 ml of precipitant solution containing 0.2 M ammonium phosphate dibasic and 20% w/v PEG 3350, equilibration against 0.2 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 2.9 A resolution Ralstonia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39183
-
x * 39183, mass spectrometry Ralstonia sp.

Organism

Organism UniProt Comment Textmining
Ralstonia sp. Q6PW32 gene pnpD
-
Ralstonia sp. SJ98 Q6PW32 gene pnpD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and SeMet-labeled PnpD from Escherichia coli BL21 (DE3) by nickel affinity chromatography Ralstonia sp.

Subunits

Subunits Comment Organism
? x * 39183, mass spectrometry Ralstonia sp.

Synonyms

Synonyms Comment Organism
PnpD
-
Ralstonia sp.