Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trichlorophenol | induction of MAR activity in wild type strain | Cupriavidus necator | |
2,4-dichlorophenoxyacetate | induction of MAR activity in wild type strain | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
the hqoD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1hqoD plasmids and transferred to Pseudomonas putida KT2442 | Cupriavidus necator |
the hxqD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1hxqD plasmids and transferred to Pseudomonas putida KT2442 | Cupriavidus necator |
the tcpD gene is cloned downstream of the arabinose-inducible promoter PBAD in the pBS1 vector to generate pBS1tcpD, plasmids and transferred to Pseudomonas putida KT2442 | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenoxyacetate + NADH | Cupriavidus necator | degradation of haloaromatic compounds | ? | - |
? | |
2,4-dichlorophenoxyacetate + NADH | Cupriavidus necator JMP 134-1 | degradation of haloaromatic compounds | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Cupriavidus necator JMP 134-1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
basal MAR activity under non-induced conditions in wild type | Cupriavidus necator |
0.332 | - |
cell extracts of Pseudomonas putida KT2442 containing the pBS1hxqD plasmid | Cupriavidus necator |
1.112 | - |
cell extracts of Pseudomonas putida KT2442 containing the pBS1hqoD plasmid | Cupriavidus necator |
5.983 | - |
cell extracts of Pseudomonas putida KT2442 containing the pBS1tcpD plasmid | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dichlorophenoxyacetate + NADH | degradation of haloaromatic compounds | Cupriavidus necator | ? | - |
? | |
2,4-dichlorophenoxyacetate + NADH | degradation of haloaromatic compounds | Cupriavidus necator JMP 134-1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HqoD | - |
Cupriavidus necator |
HqoD | gene product shows MAR activity | Cupriavidus necator |
HxqD | - |
Cupriavidus necator |
maleylacetate reductase | - |
Cupriavidus necator |
MAR | - |
Cupriavidus necator |
TcpD | - |
Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cupriavidus necator |