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Literature summary for 1.3.1.32 extracted from
- Purification and characterization of maleylacetate reductase from Nocardioides simplex 3E utilizing phenoxyalcanoic herbicides 2,4-D and 2,4,5-T (1999), Biochemistry (Moscow), 64, 625-630.
General Stability
| General Stability | Organism |
|---|---|
| enzyme is very unstable | Pimelobacter simplex |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
maleylacetate | - |
Pimelobacter simplex |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, homodimer | Pimelobacter simplex |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-maleylacetate + NADPH | Pimelobacter simplex | - |
3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | Pimelobacter simplex 3E | - |
3-oxoadipate + NADP+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pimelobacter simplex | - |
3E | - |
| Pimelobacter simplex 3E | - |
3E | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pimelobacter simplex |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-maleylacetate + NAD(P)H | - |
Pimelobacter simplex | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NAD(P)H | - |
Pimelobacter simplex 3E | 3-oxoadipate + NAD(P)+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Pimelobacter simplex | 3-oxoadipate + NADP+ | - |
ir | |
| 2-maleylacetate + NADPH | - |
Pimelobacter simplex 3E | 3-oxoadipate + NADP+ | - |
ir | |
| phenoxyalacanoic herbicide 2,4,5-T + NADH | - |
Pimelobacter simplex | ? + NAD+ | - |
? | |
| phenoxyalacanoic herbicide 2,4,5-T + NADH | - |
Pimelobacter simplex 3E | ? + NAD+ | - |
? | |
| phenoxyalacanoic herbicide 2,4-D + NADH | - |
Pimelobacter simplex | ? + NAD+ | - |
? | |
| phenoxyalacanoic herbicide 2,4-D + NADH | - |
Pimelobacter simplex 3E | ? + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 37000, homodimer | Pimelobacter simplex |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Pimelobacter simplex |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 114 | - |
maleylacetate | - |
Pimelobacter simplex | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.1 | - |
Pimelobacter simplex |
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