Literature summary for 1.3.1.31 extracted from

Gall, M.; Thomsen, M.; Peters, C.; Pavlidis, I.V.; Jonczyk, P.; Gruenert, P.P.; Beutel, S.; Scheper, T.; Gross, E.; Backes, M.; Geissler, T.; Ley, J.P.; Hilmer, J.M.; Krammer, G.; Palm, G.J.; Hinrichs, W.; Bornscheuer, U.T.
Enzymatic conversion of flavonoids using bacterial chalcone isomerase and enoate reductase (2014), Angew. Chem. Int. Ed. Engl., 53, 1439-1442.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Eubacterium ramulus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
70000	-	x * 70000, SDS-PAGE	Eubacterium ramulus
75000	-	-	Eubacterium ramulus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
chalcone + NAD+	Eubacterium ramulus	-	dihydrochalcone + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Eubacterium ramulus	V9P074	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chalcone + NAD+	-	Eubacterium ramulus	dihydrochalcone + NADH + H+	-	?
additional information	the recombinant enzyme exhibits low activity for the conversion of the naringenin into phloretin	Eubacterium ramulus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 70000, SDS-PAGE	Eubacterium ramulus
?	x * 75000, calculated from amino acid sequence	Eubacterium ramulus

Synonyms

Synonyms	Comment	Organism
enoate reductase	-	Eubacterium ramulus
ERED	-	Eubacterium ramulus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Eubacterium ramulus
NAD+	-	Eubacterium ramulus

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Release 2023.1 (January 2023)