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Literature summary for 1.3.1.31 extracted from

  • Buehler, M.; Simon, H.
    On the kinetics and mechanism of enoate reductase (1982), Hoppe-Seyler's Z. Physiol. Chem., 363, 609-625.
    View publication on PubMed
Search for more literature for 1.3.1.31

Inhibitors

Inhibitors Comment Organism Structure
3-Phenylpropionate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium kluyveri
3-Phenylpropionate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium sp.
4-phenylbutanoate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium kluyveri
4-phenylbutanoate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium sp.
benzoate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium kluyveri
benzoate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium sp.
dicoumarol blocks the NAD-binding domain Clostridium kluyveri
dicoumarol blocks the NAD-binding domain Clostridium sp.
fumarate
-
 Clostridium kluyveri
fumarate
-
 Clostridium sp.
morin blocks the NAD-binding domain Clostridium kluyveri
morin blocks the NAD-binding domain Clostridium sp.
NAD+ mixed-type inhibitor Clostridium kluyveri
NAD+ mixed-type inhibitor Clostridium sp.
phenylacetate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium kluyveri
phenylacetate inhibits reduction of (E)-2-methyl-2-butenoate Clostridium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Clostridium kluyveri
additional information
-
 additional information
-
 Clostridium sp.

Organism

Organism UniProt Comment Textmining
Clostridium kluyveri
-
-
-
Clostridium sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
butanoate + NAD+ = but-2-enoate + NADH + H+ bi bi ping pong mechanism Clostridium kluyveri
a
butanoate + NAD+ = but-2-enoate + NADH + H+ bi bi ping pong mechanism Clostridium sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-2,5-dimethoxycinnamate + NADH
-
 Clostridium sp. 3-(2,5-dimethoxyphenyl)propionate + NAD+
-
 ?
(E)-2-butenoate + NADH
-
 Clostridium kluyveri butanoate + NAD+
-
 ?
(E)-2-butenoate + NADH
-
 Clostridium sp. butanoate + NAD+
-
 ?
(E)-2-methyl-2-butenoate + NADH
-
 Clostridium kluyveri 2-methylbutanoate + NAD+
-
 ?
(E)-2-methyl-2-butenoate + NADH
-
 Clostridium sp. 2-methylbutanoate + NAD+
-
 ?
(E)-4-methyl-2-pentenoate + NADH
-
 Clostridium kluyveri 4-methylpentanoate + NAD+
-
 ?
(E)-4-methyl-2-pentenoate + NADH
-
 Clostridium sp. 4-methylpentanoate + NAD+
-
 ?
(E)-cinnamate + NADH
-
 Clostridium kluyveri phenylpropionate + NAD+
-
 ?
(E)-cinnamate + NADH
-
 Clostridium sp. phenylpropionate + NAD+
-
 ?
(E)-o-hydroxycinnamate + NADH
-
 Clostridium kluyveri 3-(2-hydroxyphenyl)propionate + NAD+
-
 ?
(E)-o-hydroxycinnamate + NADH
-
 Clostridium sp. 3-(2-hydroxyphenyl)propionate + NAD+
-
 ?
(E)-p-(dimethylamino)cinnamate + NADH
-
 Clostridium kluyveri 3-(4-(dimethylamino)phenyl)propionate + NAD+
-
 ?
(E)-p-(dimethylamino)cinnamate + NADH
-
 Clostridium sp. 3-(4-(dimethylamino)phenyl)propionate + NAD+
-
 ?
(E)-p-chlorocinnamate + NADH
-
 Clostridium kluyveri 3-(4-chlorophenyl)propionate + NAD+
-
 ?
(E)-p-chlorocinnamate + NADH
-
 Clostridium sp. 3-(4-chlorophenyl)propionate + NAD+
-
 ?
(E)-p-methoxycinnamate + NADH
-
 Clostridium kluyveri 3-(4-methoxyphenyl)propionate + NAD+
-
 ?
(E)-p-methoxycinnamate + NADH
-
 Clostridium sp. 3-(4-methoxyphenyl)propionate + NAD+
-
 ?
(E)-p-nitrocinnamate + NADH
-
 Clostridium kluyveri 3-(4-nitrophenyl)propionate + NAD+
-
 ?
(E)-p-nitrocinnamate + NADH
-
 Clostridium sp. 3-(4-nitrophenyl)propionate + NAD+
-
 ?
(E,E)-2,4-hexadienoate + NADH
-
 Clostridium sp. ? + NAD+
-
 ?
(R,S)-1-methyl-3-phenylallenecarboxylate + NADH
-
 Clostridium sp. ? + NAD+
-
 ?
(Z)-2-(formylamino)cinnamate + NADH
-
 Clostridium kluyveri 3-(4-(formylamino)phenyl)propionate + NAD+
-
 ?
(Z)-2-(formylamino)cinnamate + NADH
-
 Clostridium sp. 3-(4-(formylamino)phenyl)propionate + NAD+
-
 ?
(Z)-2-chloro-3-(p-chlorophenyl)acrylate + NADH
-
 Clostridium sp. 2-chloro-3-(4-chlorophenyl)propionate + NAD+
-
 ?
(Z)-2-fluorocinnamate + NADH
-
 Clostridium sp. 3-(2-fluorophenyl)propionate + NAD+
-
 ?
(Z)-3-chlorocinnamate + NADH
-
 Clostridium sp. 3-(3-chlorophenyl)propionate + NAD+
-
 ?
butenoate + NADH
-
 Clostridium kluyveri butanoate + NAD+
-
 ?
butenoate + NADH
-
 Clostridium sp. butanoate + NAD+
-
 ?
monomethyl fumarate + NADH
-
 Clostridium sp. 4-methoxy-4-oxobutanoate + NAD+
-
 ?
additional information transhydrogenase activity: reduction of 3-acetylpyridine adenine dinucleotide by NADH Clostridium kluyveri ?
-
 ?
additional information transhydrogenase activity: reduction of 3-acetylpyridine adenine dinucleotide by NADH Clostridium sp. ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH-optima for transhydrogenase activity Clostridium kluyveri
additional information
-
 pH-optima for transhydrogenase activity Clostridium sp.

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme splits off exclusively the (4S)-hydrogen Clostridium kluyveri
NADH the enzyme splits off exclusively the (4S)-hydrogen Clostridium sp.