Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.3 extracted from

  • Herl, V.; Fischer, G.; Bötsch, R.; Müller-Uri, F.; Kreis, W.
    Molecular cloning and expression of progesterone 5beta-reductase (5beta-POR) from Isoplexis canariensis (2006), Planta Med., 72, 1163-1165.
    View publication on PubMed

Application

Application Comment Organism
additional information significant homology to the putative progesterone 5beta-reductases isolated from other plant species, such as Digitalis lanata (ca. 98% identical) and Arabidopsis thaliana (ca. 69% identical) Digitalis canariensis

Cloned(Commentary)

Cloned (Comment) Organism
SPHI /SALI IC5beta-POR fragment cloned into the pQE vector system and transformed into Escherichai coli strain M15(pREP4) Digitalis canariensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
NADPH
-
Digitalis canariensis
0.215
-
progesterone
-
Digitalis canariensis
2.079
-
cortisol
-
Digitalis canariensis
2.193
-
4-androstene-3,17-dione
-
Digitalis canariensis
2.373
-
cortexone
-
Digitalis canariensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS–PAGE Digitalis canariensis

Organism

Organism UniProt Comment Textmining
Digitalis canariensis Q078S6
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA affinity chromatography Digitalis canariensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Digitalis canariensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-androstene-3,17-dione + NADPH
-
Digitalis canariensis 5beta-androstan-3,17-dione + NADP+
-
?
cortexone + NADPH
-
Digitalis canariensis 21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
?
cortisol + NADPH
-
Digitalis canariensis 11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
?
progesterone + NADPH + H+
-
Digitalis canariensis 5beta-pregnane-3,20-dione + NADP+
-
ir

Subunits

Subunits Comment Organism
? x * 45000, SDS–PAGE Digitalis canariensis

Synonyms

Synonyms Comment Organism
5beta-POR
-
Digitalis canariensis
EC 1.3.1.3 related Digitalis canariensis
progesterone 5beta-reductase
-
Digitalis canariensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Digitalis canariensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Digitalis canariensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Digitalis canariensis