Application | Comment | Organism |
---|---|---|
additional information | 5beta-POR is highly conserved within the genus Digitalis and the respective genes and proteins share considerable homology to putative progesterone reductases from other plant species | Digitalis lanata |
Cloned (Comment) | Organism |
---|---|
Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4) | Digitalis lanata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
NADPH | - |
Digitalis lanata | |
0.12 | - |
progesterone | - |
Digitalis lanata | |
0.228 | - |
4-androstene-3,17-dione | - |
Digitalis lanata | |
0.291 | - |
cortisol | - |
Digitalis lanata | |
1.597 | - |
cortexone | - |
Digitalis lanata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS–PAGE | Digitalis lanata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Digitalis lanata | Q6PQJ9 | - |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA affinity chromatography | Digitalis lanata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Digitalis lanata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + NADPH | - |
Digitalis lanata | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
cortexone + NADPH | - |
Digitalis lanata | 21-trihydroxy-5beta-pregnane-3,20-dione + NADP+ | - |
? | |
cortisol + NADPH | - |
Digitalis lanata | 11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+ | - |
? | |
additional information | pregnenolone, 21-OH-pregenenolone and isoprogesterone are not accepted by recombinant 5beta-POR | Digitalis lanata | ? | - |
? | |
progesterone + NADPH + H+ | 5alpha-pregnane-3,20-dione, the 5alpha-isomer of the pregnane-3,20-dione is not formed | Digitalis lanata | 5beta-pregnane-3,20-dione + NADP+ | - |
ir | |
testosterone + NADPH + H+ | - |
Digitalis lanata | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS–PAGE | Digitalis lanata |
Synonyms | Comment | Organism |
---|---|---|
5beta-POR | - |
Digitalis lanata |
progesterone 5beta-reductase | - |
Digitalis lanata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
with progesterone as substrate | Digitalis lanata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
with progesterone as substrate | Digitalis lanata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | cannot be replaced by NADH | Digitalis lanata |