Application | Comment | Organism |
---|---|---|
additional information | P5betaR belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, bearing no structural homology to its mammalian counterpart, which is a member of the aldo-keto reductase (AKR) superfamily | Digitalis purpurea |
Cloned (Comment) | Organism |
---|---|
full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag | Digitalis purpurea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
NADPH | - |
Digitalis purpurea | |
0.0391 | - |
progesterone | - |
Digitalis purpurea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43963, sequence analysis, x * 43000, purified enzyme, SDSPAGE | Digitalis purpurea |
43963 | - |
x * 43963, sequence analysis, x * 43000, purified enzyme, SDSPAGE | Digitalis purpurea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Digitalis purpurea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity by affinity chromatography | Digitalis purpurea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | maximally transcribed in mature flowers | Digitalis purpurea | - |
leaf | maximally transcribed | Digitalis purpurea | - |
root | - |
Digitalis purpurea | - |
stem | at the base of the rosette | Digitalis purpurea | - |
vascular bundle | at the basal part of the leaf, lowest amount | Digitalis purpurea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the recombinant enzyme catalyzes the stereospecific reduction of the delta4-double bond of several steroids with a 3-oxo,delta4,5 structure | Digitalis purpurea | ? | - |
? | |
progesterone + NADPH + H+ | highest substrate specificity | Digitalis purpurea | 5beta-pregnane-3,20-dione + NADP+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 43963, sequence analysis, x * 43000, purified enzyme, SDSPAGE | Digitalis purpurea |
Synonyms | Comment | Organism |
---|---|---|
P5betaR | - |
Digitalis purpurea |
progesterone 5beta-reductase | - |
Digitalis purpurea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Digitalis purpurea |