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Literature summary for 1.3.1.3 extracted from

  • Gavidia, I.; Tarrio, R.; Rodriguez-Trelles, F.; Perez-Bermudez, P.; Ulrich Seitz, H.
    Plant progesterone 5beta-reductase is not homologous to the animal enzyme. Molecular evolutionary characterization of P5betaR from Digitalis purpurea (2007), Phytochemistry, 68, 853-864.
    View publication on PubMed

Application

Application Comment Organism
additional information P5betaR belongs to the short-chain dehydrogenase/reductase (SDR) superfamily, bearing no structural homology to its mammalian counterpart, which is a member of the aldo-keto reductase (AKR) superfamily Digitalis purpurea

Cloned(Commentary)

Cloned (Comment) Organism
full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag Digitalis purpurea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0079
-
NADPH
-
Digitalis purpurea
0.0391
-
progesterone
-
Digitalis purpurea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43963, sequence analysis, x * 43000, purified enzyme, SDS–PAGE Digitalis purpurea
43963
-
x * 43963, sequence analysis, x * 43000, purified enzyme, SDS–PAGE Digitalis purpurea

Organism

Organism UniProt Comment Textmining
Digitalis purpurea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity by affinity chromatography Digitalis purpurea

Source Tissue

Source Tissue Comment Organism Textmining
flower maximally transcribed in mature flowers Digitalis purpurea
-
leaf maximally transcribed Digitalis purpurea
-
root
-
Digitalis purpurea
-
stem at the base of the rosette Digitalis purpurea
-
vascular bundle at the basal part of the leaf, lowest amount Digitalis purpurea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant enzyme catalyzes the stereospecific reduction of the delta4-double bond of several steroids with a 3-oxo,delta4,5 structure Digitalis purpurea ?
-
?
progesterone + NADPH + H+ highest substrate specificity Digitalis purpurea 5beta-pregnane-3,20-dione + NADP+
-
ir

Subunits

Subunits Comment Organism
? x * 43963, sequence analysis, x * 43000, purified enzyme, SDS–PAGE Digitalis purpurea

Synonyms

Synonyms Comment Organism
P5betaR
-
Digitalis purpurea
progesterone 5beta-reductase
-
Digitalis purpurea

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Digitalis purpurea