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Literature summary for 1.3.1.3 extracted from
- Transformation of testosterone into 17beta-hydroxy-5beta-androstan-3-one by Rana esculenta skeletal muscle (1974), Biochim. Biophys. Acta, 348, 425-437.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 17beta-hydroxy-5alpha-androstan-3-one | 0.01 mM, 57% inhibition | Pelophylax lessonae |  |
| 17beta-hydroxy-5beta-androstan-3-one | 0.01 mM, 28% inhibition | Pelophylax lessonae | |
| androstenedione | 0.01 mM, 93% inhibition | Pelophylax lessonae | |
| Cu2+ | 0.5 mM 66% inhibition | Pelophylax lessonae | |
| estradiol-17beta | 0.01 mM, 29% inhibition | Pelophylax lessonae | |
| estrone | 0.01 mM, 29% inhibition | Pelophylax lessonae | |
| Hg2+ | 0.1 mM, 92% inhibition | Pelophylax lessonae | |
| p-chloromercuribenzoate | 0.1 mM, 95% inhibition | Pelophylax lessonae | |
| progesterone | 0.002 mM, 84% inhibition | Pelophylax lessonae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
testosterone | - |
Pelophylax lessonae | |
| 0.0052 | - |
NADPH | - |
Pelophylax lessonae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Pelophylax lessonae | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pelophylax lessonae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pelophylax lessonae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| forelimb muscle | male | Pelophylax lessonae | - |
| skeletal muscle | - |
Pelophylax lessonae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000036 | - |
testosterone reduction, enzyme activity in forelimb muscle | Pelophylax lessonae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-hydroxyandrost-4-en-3-one + NADPH | trivial name testosterone | Pelophylax lessonae | 17beta-hydroxy-5beta-androstan-3-one + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Pelophylax lessonae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pelophylax lessonae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | absolutely required | Pelophylax lessonae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00024 | - |
progesterone | - |
Pelophylax lessonae | |
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