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Literature summary for 1.3.1.3 extracted from

  • Nduaguba, J.C.; Clark, A.F.
    Steroid DELTA4-reductases of pig liver: partial purification of testosterone 5beta-reductase (1971), Can. J. Biochem., 49, 385-392.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes during purification, 4°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sus scrofa 5829
-
microsome
-
Sus scrofa
-
-

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000167
-
substrate testosterone Sus scrofa
13.1
-
purified enzyme, substrate testosterone Sus scrofa

Storage Stability

Storage Stability Organism
4°C, rapid loss of activity, 2-mercaptoethanol stabilizes Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cortisone + NADPH
-
Sus scrofa ?
-
?
testosterone + NADPH + H+
-
Sus scrofa (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Sus scrofa