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Literature summary for 1.3.1.3 extracted from
- Purification and characterization of DELTA 4-3-ketosteroid 5beta-reductase (1984), J. Biol. Chem., 259, 7519-7524.
General Stability
| General Stability | Organism |
|---|---|
| highly labile in low buffer concentration | Rattus norvegicus |
| markedly stabilized in 20% glycerol | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00219 | - |
progesterone | - |
Rattus norvegicus | |
| 0.0024 | - |
testosterone | - |
Rattus norvegicus | |
| 0.003 | - |
NADPH | - |
Rattus norvegicus | |
| 0.00485 | - |
4-androstene-3,17-dione | - |
Rattus norvegicus | |
| 0.0091 | - |
7alpha-hydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
| 0.011 | - |
cortisol | - |
Rattus norvegicus | |
| 0.0122 | - |
7alpha,12alpha-dihydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
| 0.0217 | - |
cortisone | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
SDS-PAGE, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + NADPH | Rattus norvegicus | - |
5beta-androstan-3,17-dione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01528 | - |
- |
Rattus norvegicus |
| 0.0153 | - |
purified enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + NADPH | - |
Rattus norvegicus | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
| 7alpha,12alpha-dihydroxy-4-cholesten-3-one + NADPH | - |
Rattus norvegicus | 7alpha,12alpha-dihydroxy-5-beta-cholestan-3-one + NADP+ | - |
? | |
| 7alpha-hydroxy-4-cholesten + NADPH | - |
Rattus norvegicus | 7alpha-hydroxy-5beta-cholestan-3-one + NADP+ | - |
? | |
| 7alpha-hydroxy-4-cholesten-3-one + NADPH + H+ | - |
Rattus norvegicus | 7alpha-hydroxy-5beta-cholestan-3-one + NADP+ | - |
r | |
| cortisol + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| cortisone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| additional information | NADH: no effect | Rattus norvegicus | ? | - |
? | |
| progesterone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| testosterone + NADPH + H+ | - |
Rattus norvegicus | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00817 | - |
cortisol | - |
Rattus norvegicus | |
| 0.01 | - |
7alpha-hydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
| 0.0128 | - |
7alpha,12alpha-dihydroxy-4-cholesten-3-one | - |
Rattus norvegicus | |
| 0.0208 | - |
testosterone | - |
Rattus norvegicus | |
| 0.0302 | - |
progesterone | - |
Rattus norvegicus | |
| 0.0337 | - |
4-androstene-3,17-dione | - |
Rattus norvegicus | |
| 0.0375 | - |
cortisone | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
7alpha-hydroxy-4-cholesten-3-one | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.2 | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | NADH: no effect | Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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