BRENDA - Enzyme Database show
show all sequences of 1.3.1.29

A combined approach for enhancing the stability of recombinant cis-dihydrodiol naphthalene dehydrogenase from Pseudomonas putida G7 allowed for the structural and kinetic characterization of the enzyme

Costa, D.M.; Costa, M.A.; Guimaraes, S.L.; Coitinho, J.B.; Gomez, S.V.; Brandao, T.A.; Nagem, R.A.; Protein Expr. Purif. 132, 50-59 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Rosetta (DE3) cells
Pseudomonas putida
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.2 M calcium chloride dehydrate, 0.1 M HEPES sodium pH 7.5, 28% (w/v) polyethylene glycol 400
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 7.0 and 25°C
Pseudomonas putida
0.012
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 9.4 and 25°C
Pseudomonas putida
0.31
-
NAD+
at pH 9.4 and 25°C
Pseudomonas putida
0.35
-
NAD+
at pH 7.0 and 25°C
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
-
Pseudomonas putida
122000
-
His6-tagged enzyme, gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
Pseudomonas putida
-
naphthalene-1,2-diol + NADH + H+
-
-
?
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
Pseudomonas putida G7
-
naphthalene-1,2-diol + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
Q9R9Y8
-
-
Pseudomonas putida G7
Q9R9Y8
-
-
Purification (Commentary)
Commentary
Organism
HisTrap column chromatography and Superdex 200 gel filtration
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
741363
Pseudomonas putida
naphthalene-1,2-diol + NADH + H+
-
-
-
?
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
741363
Pseudomonas putida G7
naphthalene-1,2-diol + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 27000, His6-tagged enzyme, SDS-PAGE; 4 * 29520, calculated from amino acid sequence
Pseudomonas putida
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.6
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 7.0 and 25°C
Pseudomonas putida
8.3
-
NAD+
at pH 7.0 and 25°C
Pseudomonas putida
26.4
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 9.4 and 25°C
Pseudomonas putida
36.6
-
NAD+
at pH 9.4 and 25°C
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Rosetta (DE3) cells
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.2 M calcium chloride dehydrate, 0.1 M HEPES sodium pH 7.5, 28% (w/v) polyethylene glycol 400
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 7.0 and 25°C
Pseudomonas putida
0.012
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 9.4 and 25°C
Pseudomonas putida
0.31
-
NAD+
at pH 9.4 and 25°C
Pseudomonas putida
0.35
-
NAD+
at pH 7.0 and 25°C
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
-
Pseudomonas putida
122000
-
His6-tagged enzyme, gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
Pseudomonas putida
-
naphthalene-1,2-diol + NADH + H+
-
-
?
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
Pseudomonas putida G7
-
naphthalene-1,2-diol + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
HisTrap column chromatography and Superdex 200 gel filtration
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
741363
Pseudomonas putida
naphthalene-1,2-diol + NADH + H+
-
-
-
?
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
741363
Pseudomonas putida G7
naphthalene-1,2-diol + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 27000, His6-tagged enzyme, SDS-PAGE; 4 * 29520, calculated from amino acid sequence
Pseudomonas putida
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.6
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 7.0 and 25°C
Pseudomonas putida
8.3
-
NAD+
at pH 7.0 and 25°C
Pseudomonas putida
26.4
-
(1R,2S)-1,2-dihydronaphthalene-1,2-diol
at pH 9.4 and 25°C
Pseudomonas putida
36.6
-
NAD+
at pH 9.4 and 25°C
Pseudomonas putida
Other publictions for EC 1.3.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741363
Costa
A combined approach for enhanc ...
Pseudomonas putida, Pseudomonas putida G7
Protein Expr. Purif.
132
50-59
2017
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1
1
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4
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2
2
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4
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1
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2
1
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4
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1
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1
1
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2
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1
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2
1
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4
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-
-
-
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688444
Sello
-
Biocatalyst expressing cis-nap ...
Pseudomonas fluorescens, Pseudomonas fluorescens N3
J. Mol. Catal. B
52-53
67-73
2008
-
-
1
-
-
-
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-
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5
-
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2
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1
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1
1
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-
2
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688164
Silva-Stenico
Degradation of low rank coal b ...
Trichoderma atroviride, Trichoderma atroviride ES11
J. Ind. Microbiol. Biotechnol.
34
625-631
2007
-
-
-
-
-
-
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2
-
4
-
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4
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1
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1
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2
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-
4
-
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-
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-
-
-
671464
Jouanneau
Purification and characterizat ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans CHY-1
Appl. Environ. Microbiol.
72
4726-4734
2006
-
-
1
-
-
-
2
10
-
-
3
-
-
4
-
-
1
-
-
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-
-
14
1
-
-
-
11
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
10
-
-
3
-
-
-
-
1
-
-
-
-
14
1
-
-
-
11
1
1
-
-
-
-
-
-
-
-
654397
Allen
-
Selectivity studies of the ben ...
Pseudomonas putida, Pseudomonas putida 8859
Biocatal. Biotransform.
20
257-264
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
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-
1
8
-
10
-
1
-
-
-
1
-
-
1
-
-
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-
-
-
1
-
-
-
-
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-
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-
1
-
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-
1
-
1
8
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
390645
Kulakov
Cloning and characterization o ...
Rhodococcus sp., Rhodococcus sp. NCIMB12038
FEMS Microbiol. Lett.
182
327-331
2000
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-
1
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5
-
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-
1
-
2
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-
1
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1
1
-
-
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-
-
-
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-
-
1
-
2
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
246449
Barriault
cis-2,3-Dihydro-2,3-dihydroxyb ...
Pseudomonas putida, Pseudomonas putida G7
Biochem. Biophys. Res. Commun.
260
181-187
1999
-
-
-
-
-
-
-
3
-
-
-
-
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
1
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-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
390643
Sikkema
Metabolism of tetralin (1,2,3, ...
Corynebacterium sp., Corynebacterium sp. C125
Appl. Environ. Microbiol.
59
567-572
1993
-
-
-
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2
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9
-
8
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1
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-
1
-
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9
-
8
-
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390637
Serdar
Isolation and characterization ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
164
764-771
1989
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1
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1
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1
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1
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1
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1
-
1
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390638
Serdar
Studies of nucleotide sequence ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
164
772-779
1989
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-
1
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1
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3
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1
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1
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1
1
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3
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1
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390639
Durham
-
Genetic engineering in Pseudom ...
Pseudomonas putida
Dev. Ind. Microbiol.
25
163-174
1984
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-
1
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1
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1
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1
1
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2
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1
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390252
Schell
Cloning and expression in Esch ...
Pseudomonas putida
J. Bacteriol.
153
822-829
1983
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1
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1
1
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2
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1
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390642
Patel
Bacterial cis-dihydrodiol dehy ...
Nocardia sp., Pseudomonas putida
J. Bacteriol.
128
842-850
1976
-
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10
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3
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2
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5
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14
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2
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2
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10
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5
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14
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2
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2
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390641
Patel
Purification and properties of ...
Pseudomonas putida
J. Bacteriol.
119
879-888
1974
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1
4
2
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2
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7
1
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1
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1
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1
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