BRENDA - Enzyme Database
show all sequences of 1.3.1.29

Purification and characterization of an arene cis-dihydrodiol dehydrogenase endowed with broad substrate specificity toward polycyclic aromatic hydrocarbon dihydrodiols

Jouanneau, Y.; Meyer, C.; Appl. Environ. Microbiol. 72, 4726-4734 (2006)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
into pET15b and overexpressed as His-tagged protein in Escherichia coli BL21(DE3)
Novosphingobium aromaticivorans
Inhibitors
Inhibitors
Commentary
Organism
Structure
3,4-Dihydroxyphenanthrene
-
Novosphingobium aromaticivorans
NADH
-
Novosphingobium aromaticivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00141
-
naphthalene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
0.00185
-
phenanthrene 3,4-dihydrodiol
-
Novosphingobium aromaticivorans
0.002
-
anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
0.0022
-
chrysene 3,4-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
0.0035
-
biphenyl 2,3-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
0.0036
-
biphenyl 2,3-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
0.0057
-
chrysene 3,4-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
0.0071
-
benzo(a)anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
0.16
-
NAD+
at pH 7.0, with biphenyl 2,3-dihydrodiol as substrate
Novosphingobium aromaticivorans
0.168
-
NAD+
at pH 7.0, with phenanthrene 3,4-dihydrodiol
Novosphingobium aromaticivorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27731
-
27731, predicted from sequence analysis, 30000, SDS-PAGE
Novosphingobium aromaticivorans
103000
-
gel filtration
Novosphingobium aromaticivorans
110000
-
-
Novosphingobium aromaticivorans
Organism
Organism
UniProt
Commentary
Textmining
Novosphingobium aromaticivorans
-
-
-
Novosphingobium aromaticivorans CHY-1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
by Co2+-loaded immobilized metal affinity chromatography
Novosphingobium aromaticivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,3-dihydroxy-2,3-dihydrobiphenyl + NAD+
-
671464
Novosphingobium aromaticivorans
2,3-dihydroxybiphenyl + NADH + H+
-
-
-
?
2,3-dihydroxy-2,3-dihydrobiphenyl + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
2,3-dihydroxybiphenyl + NADH + H+
-
-
-
?
anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
anthracene 1,2-diol + NADH + H+
-
-
-
?
anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
anthracene 1,2-diol + NADH + H+
-
-
-
?
benzo(a)anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
benzo(a)anthracene 1,2-diol + NADH + H+
-
-
-
?
benzo(a)anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
benzo(a)anthracene 1,2-diol + NADH + H+
-
-
-
?
benzo(a)pyrene-9,10-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
9,10-dihydroxy-benzo(a)pyrene + NADH + H+
-
-
-
?
benzo(a)pyrene-9,10-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
9,10-dihydroxy-benzo(a)pyrene + NADH + H+
-
-
-
?
chrysene 3,4-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
chrysene 3,4-diol + NADH + H+
-
-
-
?
chrysene 3,4-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
chrysene 3,4-diol + NADH + H+
-
-
-
?
fluoranthene 2,3-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
2,3-dihydroxyfluoranthene + NADH + H+
-
-
-
?
naphthalene-1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
naphthalene-1,2-diol + NADH + H+
-
-
-
?
phenanthrene 3,4-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
phenanthrene 3,4-diol + NADH + H+
-
-
-
?
pyrene 4,5-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
4,5-dihydroxypyrene + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
27731, predicted from sequence analysis, 30000, SDS-PAGE
Novosphingobium aromaticivorans
Synonyms
Synonyms
Commentary
Organism
arene cis-dihydrodiol dehydrogenase
-
Novosphingobium aromaticivorans
PDDH
-
Novosphingobium aromaticivorans
polycyclic aromatic hydrocarbons dihydrodiol dehydrogenase
-
Novosphingobium aromaticivorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.25
-
fluoranthene 2,3-dihydrodiol
-
Novosphingobium aromaticivorans
6.35
-
pyrene 4,5-dihydrodiol
-
Novosphingobium aromaticivorans
7.9
-
benzo(a)pyrene dihydrodiol
-
Novosphingobium aromaticivorans
9.1
-
benzo(a)anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
16.7
-
phenanthrene 3,4-dihydrodiol
-
Novosphingobium aromaticivorans
20.3
-
chrysene 3,4-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
21.6
-
anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
24.6
-
biphenyl 2,3-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
28.2
-
naphthalene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
218
-
chrysene 3,4-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
321
-
biphenyl 2,3-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Novosphingobium aromaticivorans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
-
Novosphingobium aromaticivorans
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Novosphingobium aromaticivorans
NADP+
activity 25fold lower than with NAD+
Novosphingobium aromaticivorans
Cloned(Commentary) (protein specific)
Commentary
Organism
into pET15b and overexpressed as His-tagged protein in Escherichia coli BL21(DE3)
Novosphingobium aromaticivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Novosphingobium aromaticivorans
NADP+
activity 25fold lower than with NAD+
Novosphingobium aromaticivorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,4-Dihydroxyphenanthrene
-
Novosphingobium aromaticivorans
NADH
-
Novosphingobium aromaticivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00141
-
naphthalene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
0.00185
-
phenanthrene 3,4-dihydrodiol
-
Novosphingobium aromaticivorans
0.002
-
anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
0.0022
-
chrysene 3,4-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
0.0035
-
biphenyl 2,3-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
0.0036
-
biphenyl 2,3-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
0.0057
-
chrysene 3,4-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
0.0071
-
benzo(a)anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
0.16
-
NAD+
at pH 7.0, with biphenyl 2,3-dihydrodiol as substrate
Novosphingobium aromaticivorans
0.168
-
NAD+
at pH 7.0, with phenanthrene 3,4-dihydrodiol
Novosphingobium aromaticivorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27731
-
27731, predicted from sequence analysis, 30000, SDS-PAGE
Novosphingobium aromaticivorans
103000
-
gel filtration
Novosphingobium aromaticivorans
110000
-
-
Novosphingobium aromaticivorans
Purification (Commentary) (protein specific)
Commentary
Organism
by Co2+-loaded immobilized metal affinity chromatography
Novosphingobium aromaticivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,3-dihydroxy-2,3-dihydrobiphenyl + NAD+
-
671464
Novosphingobium aromaticivorans
2,3-dihydroxybiphenyl + NADH + H+
-
-
-
?
2,3-dihydroxy-2,3-dihydrobiphenyl + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
2,3-dihydroxybiphenyl + NADH + H+
-
-
-
?
anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
anthracene 1,2-diol + NADH + H+
-
-
-
?
anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
anthracene 1,2-diol + NADH + H+
-
-
-
?
benzo(a)anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
benzo(a)anthracene 1,2-diol + NADH + H+
-
-
-
?
benzo(a)anthracene 1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
benzo(a)anthracene 1,2-diol + NADH + H+
-
-
-
?
benzo(a)pyrene-9,10-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
9,10-dihydroxy-benzo(a)pyrene + NADH + H+
-
-
-
?
benzo(a)pyrene-9,10-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
9,10-dihydroxy-benzo(a)pyrene + NADH + H+
-
-
-
?
chrysene 3,4-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
chrysene 3,4-diol + NADH + H+
-
-
-
?
chrysene 3,4-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans CHY-1
chrysene 3,4-diol + NADH + H+
-
-
-
?
fluoranthene 2,3-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
2,3-dihydroxyfluoranthene + NADH + H+
-
-
-
?
naphthalene-1,2-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
naphthalene-1,2-diol + NADH + H+
-
-
-
?
phenanthrene 3,4-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
phenanthrene 3,4-diol + NADH + H+
-
-
-
?
pyrene 4,5-dihydrodiol + NAD+
-
671464
Novosphingobium aromaticivorans
4,5-dihydroxypyrene + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
27731, predicted from sequence analysis, 30000, SDS-PAGE
Novosphingobium aromaticivorans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.25
-
fluoranthene 2,3-dihydrodiol
-
Novosphingobium aromaticivorans
6.35
-
pyrene 4,5-dihydrodiol
-
Novosphingobium aromaticivorans
7.9
-
benzo(a)pyrene dihydrodiol
-
Novosphingobium aromaticivorans
9.1
-
benzo(a)anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
16.7
-
phenanthrene 3,4-dihydrodiol
-
Novosphingobium aromaticivorans
20.3
-
chrysene 3,4-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
21.6
-
anthracene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
24.6
-
biphenyl 2,3-dihydrodiol
at pH 7.0
Novosphingobium aromaticivorans
28.2
-
naphthalene 1,2-dihydrodiol
-
Novosphingobium aromaticivorans
218
-
chrysene 3,4-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
321
-
biphenyl 2,3-dihydrodiol
at pH 9.5
Novosphingobium aromaticivorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Novosphingobium aromaticivorans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
-
Novosphingobium aromaticivorans
Other publictions for EC 1.3.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741363
Costa
A combined approach for enhanc ...
Pseudomonas putida, Pseudomonas putida G7
Protein Expr. Purif.
132
50-59
2017
-
-
1
1
-
-
-
4
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
1
3
-
-
-
4
-
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-
1
-
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-
1
1
1
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4
-
-
2
2
-
-
-
1
-
-
-
-
2
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
688444
Sello
-
Biocatalyst expressing cis-nap ...
Pseudomonas fluorescens, Pseudomonas fluorescens N3
J. Mol. Catal. B
52-53
67-73
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
2
-
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-
-
-
-
-
1
-
-
-
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-
1
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
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-
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-
-
-
-
-
-
-
-
-
-
688164
Silva-Stenico
Degradation of low rank coal b ...
Trichoderma atroviride, Trichoderma atroviride ES11
J. Ind. Microbiol. Biotechnol.
34
625-631
2007
-
-
-
-
-
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-
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2
-
4
-
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4
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1
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1
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1
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2
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4
-
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-
-
-
-
-
-
-
-
-
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-
671464
Jouanneau
Purification and characterizat ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans CHY-1
Appl. Environ. Microbiol.
72
4726-4734
2006
-
-
1
-
-
-
2
10
-
-
3
-
-
4
-
-
1
-
-
-
-
-
14
1
3
-
-
-
11
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
10
-
-
3
-
-
-
-
1
-
-
-
-
14
1
-
-
-
11
1
1
-
-
-
-
-
-
-
-
654397
Allen
-
Selectivity studies of the ben ...
Pseudomonas putida, Pseudomonas putida 8859
Biocatal. Biotransform.
20
257-264
2002
-
-
-
-
-
-
-
-
-
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-
1
-
3
-
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1
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1
8
-
10
-
2
1
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-
1
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-
1
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-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
-
1
8
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
390645
Kulakov
Cloning and characterization o ...
Rhodococcus sp., Rhodococcus sp. NCIMB12038
FEMS Microbiol. Lett.
182
327-331
2000
-
-
1
-
-
-
-
-
-
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-
5
-
-
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-
-
1
-
2
-
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-
-
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-
1
-
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1
1
-
-
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-
-
-
-
-
-
-
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-
1
-
2
-
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-
-
-
-
-
-
-
-
-
-
-
-
246449
Barriault
cis-2,3-Dihydro-2,3-dihydroxyb ...
Pseudomonas putida, Pseudomonas putida G7
Biochem. Biophys. Res. Commun.
260
181-187
1999
-
-
-
-
-
-
-
3
-
-
-
-
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
390643
Sikkema
Metabolism of tetralin (1,2,3, ...
Corynebacterium sp., Corynebacterium sp. C125
Appl. Environ. Microbiol.
59
567-572
1993
-
-
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2
-
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-
9
-
8
-
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-
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-
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-
1
-
-
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-
-
-
1
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
9
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390637
Serdar
Isolation and characterization ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
164
764-771
1989
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-
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1
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-
1
-
1
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1
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390638
Serdar
Studies of nucleotide sequence ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
164
772-779
1989
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1
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3
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1
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390639
Durham
-
Genetic engineering in Pseudom ...
Pseudomonas putida
Dev. Ind. Microbiol.
25
163-174
1984
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1
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2
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1
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390252
Schell
Cloning and expression in Esch ...
Pseudomonas putida
J. Bacteriol.
153
822-829
1983
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1
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2
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1
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390642
Patel
Bacterial cis-dihydrodiol dehy ...
Nocardia sp., Pseudomonas putida
J. Bacteriol.
128
842-850
1976
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10
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3
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5
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14
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2
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2
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2
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2
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10
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3
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5
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14
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2
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2
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390641
Patel
Purification and properties of ...
Pseudomonas putida
J. Bacteriol.
119
879-888
1974
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2
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7
1
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4
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1
7
1
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