BRENDA - Enzyme Database
show all sequences of 1.3.1.29

Bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties

Patel, T.R.; Gibson, D.T.; J. Bacteriol. 128, 842-850 (1976)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
cis-Naphthalene dihydrodiol
-
Nocardia sp.
0.02
-
cis-Naphthalene dihydrodiol
strain NP
Pseudomonas putida
0.024
-
cis-Naphthalene dihydrodiol
strain NCIB
Pseudomonas putida
0.024
-
cis-Toluene dihydrodiol
strain biotype A grown on toluene
Pseudomonas putida
0.05
-
cis-benzene dihydrodiol
strain biotype A grown on benzene
Pseudomonas putida
0.071
-
NAD+
strain biotype A grown on toluene
Pseudomonas putida
0.079
-
NAD+
strain biotype A grown on benzene
Pseudomonas putida
0.65
-
NAD+
-
Nocardia sp.
1.02
-
NAD+
strain NP
Pseudomonas putida
1.04
-
NAD+
strain NCIB
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
92000
-
gel filtration
Nocardia sp.
112000
-
strain NCIB 9816, gel filtration
Pseudomonas putida
155000
160000
biotype A, depending on growth medium, gel filtration
Pseudomonas putida
Organism
Organism
UniProt
Commentary
Textmining
Nocardia sp.
-
-
-
Pseudomonas putida
-
; strains NP, PL6 and C84-15
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.19
-
activity in crude extracts
Nocardia sp.
0.25
-
activity in crude extracts of strain NP
Pseudomonas putida
0.42
-
activity in crude extracts of strain NCIB
Pseudomonas putida
0.62
-
activity in crude extracts of strain biotype A grown on benzene
Pseudomonas putida
0.94
-
activity in crude extracts of strain biotype A grown on toluene
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
390642
Pseudomonas putida
1,2-dihydroxynaphthalene + NADH
-
390642
Pseudomonas putida
?
cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
390642
Nocardia sp.
1,2-dihydroxynaphthalene + NADH
-
390642
Nocardia sp.
?
cis-anthracene dihydrodiol + NAD+
-
390642
Pseudomonas putida
1,2-dihydroxyanthracene + NADH
-
390642
Pseudomonas putida
?
cis-anthracene dihydrodiol + NAD+
-
390642
Nocardia sp.
1,2-dihydroxyanthracene + NADH
-
390642
Nocardia sp.
?
cis-benzene dihydrodiol + NAD+
-
390642
Pseudomonas putida
1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-benzene dihydrodiol + NAD+
-
390642
Nocardia sp.
1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
cis-biphenyl dihydrodiol + NAD+
-
390642
Pseudomonas putida
3-phenyl-1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-biphenyl dihydrodiol + NAD+
-
390642
Nocardia sp.
3-phenyl-1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
cis-ethylbenzene dihydrodiol + NAD+
-
390642
Pseudomonas putida
3-ethyl-1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-ethylbenzene dihydrodiol + NAD+
-
390642
Nocardia sp.
3-ethyl-1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
cis-phenanthrene dihydrodiol + NAD+
-
390642
Pseudomonas putida
3,4-dihydroxyphenanthrene + NADH
-
390642
Pseudomonas putida
?
cis-phenanthrene dihydrodiol + NAD+
-
390642
Nocardia sp.
3,4-dihydroxyphenanthrene + NADH
-
390642
Nocardia sp.
?
cis-toluene dihydrodiol + NAD+
-
390642
Pseudomonas putida
3-methyl-1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-toluene dihydrodiol + NAD+
-
390642
Nocardia sp.
3-methyl-1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable
Nocardia sp.
50
-
in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
-
Nocardia sp.
8.8
9
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NAD+
-
Nocardia sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NAD+
-
Nocardia sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.011
-
cis-Naphthalene dihydrodiol
-
Nocardia sp.
0.02
-
cis-Naphthalene dihydrodiol
strain NP
Pseudomonas putida
0.024
-
cis-Naphthalene dihydrodiol
strain NCIB
Pseudomonas putida
0.024
-
cis-Toluene dihydrodiol
strain biotype A grown on toluene
Pseudomonas putida
0.05
-
cis-benzene dihydrodiol
strain biotype A grown on benzene
Pseudomonas putida
0.071
-
NAD+
strain biotype A grown on toluene
Pseudomonas putida
0.079
-
NAD+
strain biotype A grown on benzene
Pseudomonas putida
0.65
-
NAD+
-
Nocardia sp.
1.02
-
NAD+
strain NP
Pseudomonas putida
1.04
-
NAD+
strain NCIB
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
92000
-
gel filtration
Nocardia sp.
112000
-
strain NCIB 9816, gel filtration
Pseudomonas putida
155000
160000
biotype A, depending on growth medium, gel filtration
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.19
-
activity in crude extracts
Nocardia sp.
0.25
-
activity in crude extracts of strain NP
Pseudomonas putida
0.42
-
activity in crude extracts of strain NCIB
Pseudomonas putida
0.62
-
activity in crude extracts of strain biotype A grown on benzene
Pseudomonas putida
0.94
-
activity in crude extracts of strain biotype A grown on toluene
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
390642
Pseudomonas putida
1,2-dihydroxynaphthalene + NADH
-
390642
Pseudomonas putida
?
cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
390642
Nocardia sp.
1,2-dihydroxynaphthalene + NADH
-
390642
Nocardia sp.
?
cis-anthracene dihydrodiol + NAD+
-
390642
Pseudomonas putida
1,2-dihydroxyanthracene + NADH
-
390642
Pseudomonas putida
?
cis-anthracene dihydrodiol + NAD+
-
390642
Nocardia sp.
1,2-dihydroxyanthracene + NADH
-
390642
Nocardia sp.
?
cis-benzene dihydrodiol + NAD+
-
390642
Pseudomonas putida
1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-benzene dihydrodiol + NAD+
-
390642
Nocardia sp.
1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
cis-biphenyl dihydrodiol + NAD+
-
390642
Pseudomonas putida
3-phenyl-1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-biphenyl dihydrodiol + NAD+
-
390642
Nocardia sp.
3-phenyl-1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
cis-ethylbenzene dihydrodiol + NAD+
-
390642
Pseudomonas putida
3-ethyl-1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-ethylbenzene dihydrodiol + NAD+
-
390642
Nocardia sp.
3-ethyl-1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
cis-phenanthrene dihydrodiol + NAD+
-
390642
Pseudomonas putida
3,4-dihydroxyphenanthrene + NADH
-
390642
Pseudomonas putida
?
cis-phenanthrene dihydrodiol + NAD+
-
390642
Nocardia sp.
3,4-dihydroxyphenanthrene + NADH
-
390642
Nocardia sp.
?
cis-toluene dihydrodiol + NAD+
-
390642
Pseudomonas putida
3-methyl-1,2-dihydroxybenzene + NADH
-
390642
Pseudomonas putida
?
cis-toluene dihydrodiol + NAD+
-
390642
Nocardia sp.
3-methyl-1,2-dihydroxybenzene + NADH
-
390642
Nocardia sp.
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable
Nocardia sp.
50
-
in cell extract: Pseudomonas putida NP stable, other Pseudomonas strains and Nocardia sp. unstable
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
-
Nocardia sp.
8.8
9
-
Pseudomonas putida
Other publictions for EC 1.3.1.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741363
Costa
A combined approach for enhanc ...
Pseudomonas putida, Pseudomonas putida G7
Protein Expr. Purif.
132
50-59
2017
-
-
1
1
-
-
-
4
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
1
3
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
4
-
-
2
2
-
-
-
1
-
-
-
-
2
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
688444
Sello
-
Biocatalyst expressing cis-nap ...
Pseudomonas fluorescens, Pseudomonas fluorescens N3
J. Mol. Catal. B
52-53
67-73
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688164
Silva-Stenico
Degradation of low rank coal b ...
Trichoderma atroviride, Trichoderma atroviride ES11
J. Ind. Microbiol. Biotechnol.
34
625-631
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671464
Jouanneau
Purification and characterizat ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans CHY-1
Appl. Environ. Microbiol.
72
4726-4734
2006
-
-
1
-
-
-
2
10
-
-
3
-
-
4
-
-
1
-
-
-
-
-
14
1
3
-
-
-
11
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
10
-
-
3
-
-
-
-
1
-
-
-
-
14
1
-
-
-
11
1
1
-
-
-
-
-
-
-
-
654397
Allen
-
Selectivity studies of the ben ...
Pseudomonas putida, Pseudomonas putida 8859
Biocatal. Biotransform.
20
257-264
2002
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
1
8
-
10
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
8
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
390645
Kulakov
Cloning and characterization o ...
Rhodococcus sp., Rhodococcus sp. NCIMB12038
FEMS Microbiol. Lett.
182
327-331
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
246449
Barriault
cis-2,3-Dihydro-2,3-dihydroxyb ...
Pseudomonas putida, Pseudomonas putida G7
Biochem. Biophys. Res. Commun.
260
181-187
1999
-
-
-
-
-
-
-
3
-
-
-
-
-
7
-
-
-
-
-
-
-
-
6
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
390643
Sikkema
Metabolism of tetralin (1,2,3, ...
Corynebacterium sp., Corynebacterium sp. C125
Appl. Environ. Microbiol.
59
567-572
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
9
-
8
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390637
Serdar
Isolation and characterization ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
164
764-771
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390638
Serdar
Studies of nucleotide sequence ...
Pseudomonas putida
Biochem. Biophys. Res. Commun.
164
772-779
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390639
Durham
-
Genetic engineering in Pseudom ...
Pseudomonas putida
Dev. Ind. Microbiol.
25
163-174
1984
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390252
Schell
Cloning and expression in Esch ...
Pseudomonas putida
J. Bacteriol.
153
822-829
1983
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390642
Patel
Bacterial cis-dihydrodiol dehy ...
Nocardia sp., Pseudomonas putida
J. Bacteriol.
128
842-850
1976
-
-
-
-
-
-
-
10
-
-
3
-
-
2
-
-
-
-
-
-
5
-
14
-
-
-
-
2
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
10
-
-
3
-
-
-
-
-
-
-
5
-
14
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
390641
Patel
Purification and properties of ...
Pseudomonas putida
J. Bacteriol.
119
879-888
1974
-
-
-
-
-
1
4
2
-
-
2
-
-
1
-
-
1
-
-
-
2
1
7
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
4
-
2
-
-
2
-
-
-
-
1
-
-
2
1
7
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-